High-Throughput Phototactic Ecotoxicity Biotests with Nauplii of <i>Artemia franciscana</i>
Analysis of sensorimotor behavioral responses to stimuli such as light can provide an enhanced relevance during rapid prioritisation of chemical risk. Due to technical limitations, there have been, however, only minimal studies on using invertebrate phototactic behaviors in aquatic ecotoxicity testi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Toxics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2305-6304/10/9/508 |
| _version_ | 1797481832907800576 |
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| author | Yutao Bai Jason Henry Tomasz M. Karpiński Donald Wlodkowic |
| author_facet | Yutao Bai Jason Henry Tomasz M. Karpiński Donald Wlodkowic |
| author_sort | Yutao Bai |
| collection | DOAJ |
| description | Analysis of sensorimotor behavioral responses to stimuli such as light can provide an enhanced relevance during rapid prioritisation of chemical risk. Due to technical limitations, there have been, however, only minimal studies on using invertebrate phototactic behaviors in aquatic ecotoxicity testing. In this work, we demonstrate an innovative, purpose-built analytical system for a high-throughput phototactic biotest with nauplii of euryhaline brine shrimp <i>Artemia franciscana</i>. We also, for the first time, present a novel and dedicated bioinformatic approach that facilitates high-throughput analysis of phototactic behaviors at scale with great fidelity. The nauplii exhibited consistent light-seeking behaviors upon extinguishing a brief programmable light stimulus (5500K, 400 lux) without habituation. A proof-of-concept validation involving the short-term exposure of eggs (24 h) and instar I larval stages (6 h) to sub-lethal concentrations of insecticides organophosphate chlorpyrifos (10 µg/L) and neonicotinoid imidacloprid (50 µg/L) showed perturbation in light seeking behaviors in the absence of or minimal alteration in general mobility. Our preliminary data further support the notion that phototactic bioassays can represent an attractive new avenue in behavioral ecotoxicology because of their potential sensitivity, responsiveness, and low cost. |
| first_indexed | 2024-03-09T22:20:11Z |
| format | Article |
| id | doaj.art-670c7e212a2d421a817d8829f9dc35ff |
| institution | Directory Open Access Journal |
| issn | 2305-6304 |
| language | English |
| last_indexed | 2024-03-09T22:20:11Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxics |
| spelling | doaj.art-670c7e212a2d421a817d8829f9dc35ff2023-11-23T19:15:19ZengMDPI AGToxics2305-63042022-08-0110950810.3390/toxics10090508High-Throughput Phototactic Ecotoxicity Biotests with Nauplii of <i>Artemia franciscana</i>Yutao Bai0Jason Henry1Tomasz M. Karpiński2Donald Wlodkowic3The Neurotox Lab, School of Science, RMIT University, Plenty Road, P.O. Box 71, Bundoora, VIC 3083, AustraliaThe Neurotox Lab, School of Science, RMIT University, Plenty Road, P.O. Box 71, Bundoora, VIC 3083, AustraliaChair and Department of Medical Microbiology, Poznań University of Medical Sciences, Wieniawskiego 3, 61-712 Poznań, PolandThe Neurotox Lab, School of Science, RMIT University, Plenty Road, P.O. Box 71, Bundoora, VIC 3083, AustraliaAnalysis of sensorimotor behavioral responses to stimuli such as light can provide an enhanced relevance during rapid prioritisation of chemical risk. Due to technical limitations, there have been, however, only minimal studies on using invertebrate phototactic behaviors in aquatic ecotoxicity testing. In this work, we demonstrate an innovative, purpose-built analytical system for a high-throughput phototactic biotest with nauplii of euryhaline brine shrimp <i>Artemia franciscana</i>. We also, for the first time, present a novel and dedicated bioinformatic approach that facilitates high-throughput analysis of phototactic behaviors at scale with great fidelity. The nauplii exhibited consistent light-seeking behaviors upon extinguishing a brief programmable light stimulus (5500K, 400 lux) without habituation. A proof-of-concept validation involving the short-term exposure of eggs (24 h) and instar I larval stages (6 h) to sub-lethal concentrations of insecticides organophosphate chlorpyrifos (10 µg/L) and neonicotinoid imidacloprid (50 µg/L) showed perturbation in light seeking behaviors in the absence of or minimal alteration in general mobility. Our preliminary data further support the notion that phototactic bioassays can represent an attractive new avenue in behavioral ecotoxicology because of their potential sensitivity, responsiveness, and low cost.https://www.mdpi.com/2305-6304/10/9/508ecotoxicologybehaviorphototaxis<i>Artemia franciscana</i>brine shrimp |
| spellingShingle | Yutao Bai Jason Henry Tomasz M. Karpiński Donald Wlodkowic High-Throughput Phototactic Ecotoxicity Biotests with Nauplii of <i>Artemia franciscana</i> Toxics ecotoxicology behavior phototaxis <i>Artemia franciscana</i> brine shrimp |
| title | High-Throughput Phototactic Ecotoxicity Biotests with Nauplii of <i>Artemia franciscana</i> |
| title_full | High-Throughput Phototactic Ecotoxicity Biotests with Nauplii of <i>Artemia franciscana</i> |
| title_fullStr | High-Throughput Phototactic Ecotoxicity Biotests with Nauplii of <i>Artemia franciscana</i> |
| title_full_unstemmed | High-Throughput Phototactic Ecotoxicity Biotests with Nauplii of <i>Artemia franciscana</i> |
| title_short | High-Throughput Phototactic Ecotoxicity Biotests with Nauplii of <i>Artemia franciscana</i> |
| title_sort | high throughput phototactic ecotoxicity biotests with nauplii of i artemia franciscana i |
| topic | ecotoxicology behavior phototaxis <i>Artemia franciscana</i> brine shrimp |
| url | https://www.mdpi.com/2305-6304/10/9/508 |
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