| Summary: | Cardiovascular diseases (CVDs) have posed serious public health problems,
accounting for nearly 30% of mortality worldwide and their incidence is
still increasing. Therefore, new treatment resources are necessary to prevent or
manage the ever-increasing population of patients with CVDs. Sea cucumber is
well known for its medical and health benefit effects, but it is not well known
what/how effect it has on vascular disease. In the present study, we examined
the protect effect of sea cucumber, Apostichopus japonicus
80% ethanol extract (AJE) on zebrafish embryo with the stimulation of
free fatty acid, palmitate (PA). In vivo study showed that AJE
can attenuate PA-induced toxicity through relieving the rapid heartbeat,
increasing the survival rate and reducing the malformation in both wild type and
Tg (flila:eGFP) transgenic zebrafish lines. Additionally,
compare with PA treated embryos, the yolk sac area, body length, axial vascular
segment (AVS) and intersegmental vessel (ISV) of the co-treatment group of AJE
and PA were comparable to the control group. Moreover, AJE lowered the
expression of inducible nitric oxide synthase (iNOS), nitric oxide (NO) and
inflammation-related genes induced by PA, and inhibited PA-induced vascular
development disorders. Our data preliminarily verify that AJE could be a
candidate resource for the prevention or therapy of CVDs.
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