Water extract of lotus leaves has hepatoprotective activity by enhancing Nrf2- and epigenetics-mediated cellular antioxidant capacity in mouse hepatocytes
The liver is an important organ in the human body; however, studies have shown that the excessive intake of acetaminophen (APAP), the primary ingredient in marketing pain relievers, may cause oxidative damage to liver cells. Here we found that lotus leaf water extract (LL-WE) enriched with various p...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464622004017 |
| _version_ | 1828167934060527616 |
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| author | Zheng-Yuan Su Bo-An Lai Zi-Han Lin Guor-Jien Wei Ssu-Han Huang Yen-Chen Tung Tien-Yuan Wu Jong Hun Lee Yu-Chun Hsu |
| author_facet | Zheng-Yuan Su Bo-An Lai Zi-Han Lin Guor-Jien Wei Ssu-Han Huang Yen-Chen Tung Tien-Yuan Wu Jong Hun Lee Yu-Chun Hsu |
| author_sort | Zheng-Yuan Su |
| collection | DOAJ |
| description | The liver is an important organ in the human body; however, studies have shown that the excessive intake of acetaminophen (APAP), the primary ingredient in marketing pain relievers, may cause oxidative damage to liver cells. Here we found that lotus leaf water extract (LL-WE) enriched with various phenolic compounds can prevent mice AML-12 hepatocytes from APAP-induced injury, and trigger the Nrf2 pathway in HepG2-C8 cells with ARE-luciferase plasmid. LL-WE effectively induced Nrf2 nuclear translocation and glutathione synthesis, increased gene expression of antioxidant enzymes (HO-1, NQO1, and UGT1A), and reduced protein levels of epigenetic modification enzymes (HDACs and DNMTs) in AML-12 cells. LL-WE also inhibited the activity of M.SssI CpG methyltransferase in vitro. These results suggest that LL-WE can be a potential hepatoprotective agent to reduce oxidative stress-induced liver damage by activating the Nrf2 pathway and downregulating epigenetic modification enzymes. |
| first_indexed | 2024-04-12T02:25:35Z |
| format | Article |
| id | doaj.art-6710d36ea4074696ae913a4439782863 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-04-12T02:25:35Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-6710d36ea4074696ae913a44397828632022-12-22T03:52:00ZengElsevierJournal of Functional Foods1756-46462022-12-0199105331Water extract of lotus leaves has hepatoprotective activity by enhancing Nrf2- and epigenetics-mediated cellular antioxidant capacity in mouse hepatocytesZheng-Yuan Su0Bo-An Lai1Zi-Han Lin2Guor-Jien Wei3Ssu-Han Huang4Yen-Chen Tung5Tien-Yuan Wu6Jong Hun Lee7Yu-Chun Hsu8Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan City 320314, Taiwan; Corresponding authors at: Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan City 320314, Taiwan. Division of Gastroenterology, Department of Internal Medicine, Changhua Christian Hospital, Changhua 500006, Taiwan.Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan City 320314, TaiwanDepartment of Bioscience Technology, Chung Yuan Christian University, Taoyuan City 320314, TaiwanInstitute of Food Safety and Health Risk Assessment, National Yang Ming Chiao Tung University, Taipei 112304, TaiwanDepartment of Bioscience Technology, Chung Yuan Christian University, Taoyuan City 320314, TaiwanDepartment of Food Science, National Ilan University, Yilan County 260007, TaiwanDepartment of Pharmacology, School of Medicine, College of Medicine, Tzu Chi University, Hualien 970374, TaiwanDepartment of Food Science and Biotechnology, Gachon University, Seongnam 13120, South KoreaDivision of Gastroenterology, Department of Internal Medicine, Changhua Christian Hospital, Changhua 500006, Taiwan; Corresponding authors at: Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan City 320314, Taiwan. Division of Gastroenterology, Department of Internal Medicine, Changhua Christian Hospital, Changhua 500006, Taiwan.The liver is an important organ in the human body; however, studies have shown that the excessive intake of acetaminophen (APAP), the primary ingredient in marketing pain relievers, may cause oxidative damage to liver cells. Here we found that lotus leaf water extract (LL-WE) enriched with various phenolic compounds can prevent mice AML-12 hepatocytes from APAP-induced injury, and trigger the Nrf2 pathway in HepG2-C8 cells with ARE-luciferase plasmid. LL-WE effectively induced Nrf2 nuclear translocation and glutathione synthesis, increased gene expression of antioxidant enzymes (HO-1, NQO1, and UGT1A), and reduced protein levels of epigenetic modification enzymes (HDACs and DNMTs) in AML-12 cells. LL-WE also inhibited the activity of M.SssI CpG methyltransferase in vitro. These results suggest that LL-WE can be a potential hepatoprotective agent to reduce oxidative stress-induced liver damage by activating the Nrf2 pathway and downregulating epigenetic modification enzymes.http://www.sciencedirect.com/science/article/pii/S1756464622004017AcetaminophenEpigeneticsHepatotoxicityLiverLotus leafNrf2 |
| spellingShingle | Zheng-Yuan Su Bo-An Lai Zi-Han Lin Guor-Jien Wei Ssu-Han Huang Yen-Chen Tung Tien-Yuan Wu Jong Hun Lee Yu-Chun Hsu Water extract of lotus leaves has hepatoprotective activity by enhancing Nrf2- and epigenetics-mediated cellular antioxidant capacity in mouse hepatocytes Journal of Functional Foods Acetaminophen Epigenetics Hepatotoxicity Liver Lotus leaf Nrf2 |
| title | Water extract of lotus leaves has hepatoprotective activity by enhancing Nrf2- and epigenetics-mediated cellular antioxidant capacity in mouse hepatocytes |
| title_full | Water extract of lotus leaves has hepatoprotective activity by enhancing Nrf2- and epigenetics-mediated cellular antioxidant capacity in mouse hepatocytes |
| title_fullStr | Water extract of lotus leaves has hepatoprotective activity by enhancing Nrf2- and epigenetics-mediated cellular antioxidant capacity in mouse hepatocytes |
| title_full_unstemmed | Water extract of lotus leaves has hepatoprotective activity by enhancing Nrf2- and epigenetics-mediated cellular antioxidant capacity in mouse hepatocytes |
| title_short | Water extract of lotus leaves has hepatoprotective activity by enhancing Nrf2- and epigenetics-mediated cellular antioxidant capacity in mouse hepatocytes |
| title_sort | water extract of lotus leaves has hepatoprotective activity by enhancing nrf2 and epigenetics mediated cellular antioxidant capacity in mouse hepatocytes |
| topic | Acetaminophen Epigenetics Hepatotoxicity Liver Lotus leaf Nrf2 |
| url | http://www.sciencedirect.com/science/article/pii/S1756464622004017 |
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