<it>CD7 </it>in acute myeloid leukemia: correlation with loss of wild-type <it>CEBPA</it>, consequence of epigenetic regulation
<p>Abstract</p> <p>Background</p> <p>CD7 is a negative prognostic marker in myeloid malignancies. In acute myeloid leukemia (AML), an inverse correlation exists between expression of wild-type <it>CEBPA </it>and <it>CD7</it>. Aim of this study wa...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-04-01
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| Series: | Journal of Hematology & Oncology |
| Online Access: | http://www.jhoonline.org/content/3/1/15 |
| _version_ | 1811286426218659840 |
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| author | Drexler Hans G Zaborski Margarete Romani Julia Scherr Michaela Röhrs Sonja Quentmeier Hilmar |
| author_facet | Drexler Hans G Zaborski Margarete Romani Julia Scherr Michaela Röhrs Sonja Quentmeier Hilmar |
| author_sort | Drexler Hans G |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>CD7 is a negative prognostic marker in myeloid malignancies. In acute myeloid leukemia (AML), an inverse correlation exists between expression of wild-type <it>CEBPA </it>and <it>CD7</it>. Aim of this study was to find out whether C/EBPα is a negative regulator of <it>CD7 </it>and which other regulatory mechanisms might be involved.</p> <p>Results</p> <p>As already described for primary AML cells, the majority of AML cell lines tested were either C/EBPα<sup>+</sup>/CD7<sup>- </sup>or C/EBPα<sup>-</sup>/CD7<sup>+</sup>. However, the existence of isolated CD7<sup>+ </sup>cell lines expressing wild-type C/EBPα challenges the notion that C/EBPα acts as a unique repressor of <it>CD7</it>. Furthermore, ectopic expression of <it>CEBPA </it>did not reduce <it>CD7 </it>in CD7<sup>+ </sup>cells and knock-down of C/EBPα failed to induce <it>CD7 </it>in CD7<sup>- </sup>cells. In contrast, the DNA demethylating agent Aza-2'deoxycytidine triggered <it>CD7 </it>expression in CD7<sup>- </sup>AML and in T-cell lines suggesting epigenetic regulation of <it>CD7</it>. Bisulfite sequencing data confirmed that CpGs in the <it>CD7 </it>exon1 region are methylated in CD7<sup>- </sup>cell lines, and unmethylated in CD7<sup>+ </sup>cell lines.</p> <p>Conclusion</p> <p>We confirmed an inverse correlation between the expression of wild-type <it>CEBPA </it>and of <it>CD7 </it>in AML cells. Our results contradict the hypothesis that C/EBPα acts as repressor for <it>CD7</it>, and instead show that epigenetic mechanisms are responsible for <it>CD7 </it>regulation, in AML cells as well as in T-cells, the typical CD7 expressing cell type.</p> |
| first_indexed | 2024-04-13T02:59:46Z |
| format | Article |
| id | doaj.art-67141517737840d498272e756d1a2170 |
| institution | Directory Open Access Journal |
| issn | 1756-8722 |
| language | English |
| last_indexed | 2024-04-13T02:59:46Z |
| publishDate | 2010-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Hematology & Oncology |
| spelling | doaj.art-67141517737840d498272e756d1a21702022-12-22T03:05:28ZengBMCJournal of Hematology & Oncology1756-87222010-04-01311510.1186/1756-8722-3-15<it>CD7 </it>in acute myeloid leukemia: correlation with loss of wild-type <it>CEBPA</it>, consequence of epigenetic regulationDrexler Hans GZaborski MargareteRomani JuliaScherr MichaelaRöhrs SonjaQuentmeier Hilmar<p>Abstract</p> <p>Background</p> <p>CD7 is a negative prognostic marker in myeloid malignancies. In acute myeloid leukemia (AML), an inverse correlation exists between expression of wild-type <it>CEBPA </it>and <it>CD7</it>. Aim of this study was to find out whether C/EBPα is a negative regulator of <it>CD7 </it>and which other regulatory mechanisms might be involved.</p> <p>Results</p> <p>As already described for primary AML cells, the majority of AML cell lines tested were either C/EBPα<sup>+</sup>/CD7<sup>- </sup>or C/EBPα<sup>-</sup>/CD7<sup>+</sup>. However, the existence of isolated CD7<sup>+ </sup>cell lines expressing wild-type C/EBPα challenges the notion that C/EBPα acts as a unique repressor of <it>CD7</it>. Furthermore, ectopic expression of <it>CEBPA </it>did not reduce <it>CD7 </it>in CD7<sup>+ </sup>cells and knock-down of C/EBPα failed to induce <it>CD7 </it>in CD7<sup>- </sup>cells. In contrast, the DNA demethylating agent Aza-2'deoxycytidine triggered <it>CD7 </it>expression in CD7<sup>- </sup>AML and in T-cell lines suggesting epigenetic regulation of <it>CD7</it>. Bisulfite sequencing data confirmed that CpGs in the <it>CD7 </it>exon1 region are methylated in CD7<sup>- </sup>cell lines, and unmethylated in CD7<sup>+ </sup>cell lines.</p> <p>Conclusion</p> <p>We confirmed an inverse correlation between the expression of wild-type <it>CEBPA </it>and of <it>CD7 </it>in AML cells. Our results contradict the hypothesis that C/EBPα acts as repressor for <it>CD7</it>, and instead show that epigenetic mechanisms are responsible for <it>CD7 </it>regulation, in AML cells as well as in T-cells, the typical CD7 expressing cell type.</p>http://www.jhoonline.org/content/3/1/15 |
| spellingShingle | Drexler Hans G Zaborski Margarete Romani Julia Scherr Michaela Röhrs Sonja Quentmeier Hilmar <it>CD7 </it>in acute myeloid leukemia: correlation with loss of wild-type <it>CEBPA</it>, consequence of epigenetic regulation Journal of Hematology & Oncology |
| title | <it>CD7 </it>in acute myeloid leukemia: correlation with loss of wild-type <it>CEBPA</it>, consequence of epigenetic regulation |
| title_full | <it>CD7 </it>in acute myeloid leukemia: correlation with loss of wild-type <it>CEBPA</it>, consequence of epigenetic regulation |
| title_fullStr | <it>CD7 </it>in acute myeloid leukemia: correlation with loss of wild-type <it>CEBPA</it>, consequence of epigenetic regulation |
| title_full_unstemmed | <it>CD7 </it>in acute myeloid leukemia: correlation with loss of wild-type <it>CEBPA</it>, consequence of epigenetic regulation |
| title_short | <it>CD7 </it>in acute myeloid leukemia: correlation with loss of wild-type <it>CEBPA</it>, consequence of epigenetic regulation |
| title_sort | it cd7 it in acute myeloid leukemia correlation with loss of wild type it cebpa it consequence of epigenetic regulation |
| url | http://www.jhoonline.org/content/3/1/15 |
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