Generating Paclitaxel-Resistant in Cervical Cancer HeLa Cell Line
Cervical cancer is one of the most leading causes of women death. Currently, paclitaxel is still one of the main therapeutic regimens for cervical cancer patients. However, some patients developed to be paclitaxel-resistant. Hence, studies to find out the novel strategies to resolve this problem are...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Indonesian Society for Cancer Chemoprevention
2020-07-01
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| Series: | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| Online Access: | https://ijcc.chemoprev.org/index.php/ijcc/article/view/318 |
| _version_ | 1818250500345167872 |
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| author | Muhammad Hasan Bashari Fachreza Aryo Damara Isna Nisrina Hardani Gita Widya Pradini Tenny Putri Eko Fuji Ariyanto |
| author_facet | Muhammad Hasan Bashari Fachreza Aryo Damara Isna Nisrina Hardani Gita Widya Pradini Tenny Putri Eko Fuji Ariyanto |
| author_sort | Muhammad Hasan Bashari |
| collection | DOAJ |
| description | Cervical cancer is one of the most leading causes of women death. Currently, paclitaxel is still one of the main therapeutic regimens for cervical cancer patients. However, some patients developed to be paclitaxel-resistant. Hence, studies to find out the novel strategies to resolve this problem are important. Generating resistant cancer cell lines can be utilized as the potent tool to evaluate the efficacy of any therapeutic agent toward cancer drug-resistant problems. Current studies describing the methods to establish chemoresistance are lacking. Moreover, study in Indonesia conducting chemoresistance in cell line is limited. This study was aimed to elaborate the characteristics of HeLa cells during generation of paclitaxel-resistant cervical cancer cells. The parental HeLa cells were exposed to an escalating concentration of paclitaxel for a long time period. Subsequently, cells were divided into two groups for the evaluation of resistance characteristics. The values of inhibitory concentration 50 (IC50) and inhibitory concentration 90 (IC90) were analyzed using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. Our data showed that the longer exposing periods of paclitaxel, the higher IC50 and IC90 values of HeLa cells are. IC90 of paclitaxel in HeLa Pac RB was increased from 69 pM, 440 pM, 2,561 pM and 10,337 pM on 0th, 1st, 2nd, 3rd and 4th months, respectively. Interestingly, the resistant cells were recovered to be paclitaxel-sensitive when they were not being continuously exposed to paclitaxel. In addition, the paclitaxel resistant cells become less sensitive against 5-FU but not doxorubicin, cisplatin and etoposide. We were able to generate cervical cancer HeLa paclitaxel-resistant cell line. These cell line could potentially be utilized for further studies in order to understand the molecular mechanisms of drug resistance in cervical cancer and as a tool for cancer drug discovery.
Keywords: cervical cancer, drug resistant cell line, paclitaxel resistant cells, stepwise escalating concentration. |
| first_indexed | 2024-12-12T15:53:22Z |
| format | Article |
| id | doaj.art-671431f5eb474684babbecd190136825 |
| institution | Directory Open Access Journal |
| issn | 2088-0197 2355-8989 |
| language | English |
| last_indexed | 2024-12-12T15:53:22Z |
| publishDate | 2020-07-01 |
| publisher | Indonesian Society for Cancer Chemoprevention |
| record_format | Article |
| series | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| spelling | doaj.art-671431f5eb474684babbecd1901368252022-12-22T00:19:33ZengIndonesian Society for Cancer ChemopreventionISCC (Indonesian Journal of Cancer Chemoprevention)2088-01972355-89892020-07-01112909610.14499/indonesianjcanchemoprev11iss2pp90-96207Generating Paclitaxel-Resistant in Cervical Cancer HeLa Cell LineMuhammad Hasan Bashari0Fachreza Aryo Damara1Isna Nisrina Hardani2Gita Widya Pradini3Tenny Putri4Eko Fuji Ariyanto5Department of Biomedical Sciences, Division of Pharmacology and Therapy, Faculty of Medicine, Universitas PadjadjaranUndergraduate Medical Program, Faculty of Medicine, Universitas Padjadjaran, BandungUndergraduate Medical Program, Faculty of Medicine, Universitas Padjadjaran, BandungOncology and Stem Cell Research Group, Faculty of Medicine, Universitas Padjadjaran, BandungLaboratory of cell culture and cytogenetic, Faculty of Medicine, Universitas Padjadjaran, BandungDepartment of Biomedical Sciences, Division of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Padjadjaran, BandungCervical cancer is one of the most leading causes of women death. Currently, paclitaxel is still one of the main therapeutic regimens for cervical cancer patients. However, some patients developed to be paclitaxel-resistant. Hence, studies to find out the novel strategies to resolve this problem are important. Generating resistant cancer cell lines can be utilized as the potent tool to evaluate the efficacy of any therapeutic agent toward cancer drug-resistant problems. Current studies describing the methods to establish chemoresistance are lacking. Moreover, study in Indonesia conducting chemoresistance in cell line is limited. This study was aimed to elaborate the characteristics of HeLa cells during generation of paclitaxel-resistant cervical cancer cells. The parental HeLa cells were exposed to an escalating concentration of paclitaxel for a long time period. Subsequently, cells were divided into two groups for the evaluation of resistance characteristics. The values of inhibitory concentration 50 (IC50) and inhibitory concentration 90 (IC90) were analyzed using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. Our data showed that the longer exposing periods of paclitaxel, the higher IC50 and IC90 values of HeLa cells are. IC90 of paclitaxel in HeLa Pac RB was increased from 69 pM, 440 pM, 2,561 pM and 10,337 pM on 0th, 1st, 2nd, 3rd and 4th months, respectively. Interestingly, the resistant cells were recovered to be paclitaxel-sensitive when they were not being continuously exposed to paclitaxel. In addition, the paclitaxel resistant cells become less sensitive against 5-FU but not doxorubicin, cisplatin and etoposide. We were able to generate cervical cancer HeLa paclitaxel-resistant cell line. These cell line could potentially be utilized for further studies in order to understand the molecular mechanisms of drug resistance in cervical cancer and as a tool for cancer drug discovery. Keywords: cervical cancer, drug resistant cell line, paclitaxel resistant cells, stepwise escalating concentration.https://ijcc.chemoprev.org/index.php/ijcc/article/view/318 |
| spellingShingle | Muhammad Hasan Bashari Fachreza Aryo Damara Isna Nisrina Hardani Gita Widya Pradini Tenny Putri Eko Fuji Ariyanto Generating Paclitaxel-Resistant in Cervical Cancer HeLa Cell Line ISCC (Indonesian Journal of Cancer Chemoprevention) |
| title | Generating Paclitaxel-Resistant in Cervical Cancer HeLa Cell Line |
| title_full | Generating Paclitaxel-Resistant in Cervical Cancer HeLa Cell Line |
| title_fullStr | Generating Paclitaxel-Resistant in Cervical Cancer HeLa Cell Line |
| title_full_unstemmed | Generating Paclitaxel-Resistant in Cervical Cancer HeLa Cell Line |
| title_short | Generating Paclitaxel-Resistant in Cervical Cancer HeLa Cell Line |
| title_sort | generating paclitaxel resistant in cervical cancer hela cell line |
| url | https://ijcc.chemoprev.org/index.php/ijcc/article/view/318 |
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