In vivo and ex vivo measurements: noninvasive assessment of alcoholic fatty liver using 1H-MR spectroscopy
PURPOSEWe aimed to evaluate the ability of 1H-magnetic resonance spectroscopy (1H-MRS) to detect and quantify hepatic fat content in vivo and ex vivo in an experimental rat model of alcoholic fatty liver using histopathology, biochemistry, and laboratory analyses as reference.METHODSAlcoholic fatty...
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2016-01-01
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| Series: | Diagnostic and Interventional Radiology |
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http://www.dirjournal.org/archives/archive-detail/article-preview/n-vivo-and-ex-vivo-measurements-noninvasive-assess/55531
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| _version_ | 1797691213581647872 |
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| author | Daniel Keese Hüdayi Korkusuz Frank Huebner Dmitry Namgaladze Bahram Raschidi Thomas J. Vogl |
| author_facet | Daniel Keese Hüdayi Korkusuz Frank Huebner Dmitry Namgaladze Bahram Raschidi Thomas J. Vogl |
| author_sort | Daniel Keese |
| collection | DOAJ |
| description | PURPOSEWe aimed to evaluate the ability of 1H-magnetic resonance spectroscopy (1H-MRS) to detect and quantify hepatic fat content in vivo and ex vivo in an experimental rat model of alcoholic fatty liver using histopathology, biochemistry, and laboratory analyses as reference.METHODSAlcoholic fatty liver was induced within 48 hours in 20 Lewis rats; 10 rats served as control. Intrahepatic fat content determined by 1H-MRS was expressed as the percent ratio of the lipid and water peaks and was correlated with intrahepatic fat content determined histologically and biochemically. Liver enzymes were measured in serum.RESULTSFatty liver could be detected in vivo as well as ex vivo using 1H-MRS, in all 20 animals. Histologic analysis showed a fatty liver in 16 of 20 animals. Histology and 1H-MRS results were highly correlated (in vivo, r=0.93, P = 0.0005; ex vivo, r=0.92, P = 0.0006). Also a strong correlation was noted between in vivo 1H-MRS measurements and the fat content determined biochemically (r=0.96, P = 0.0003). Ex vivo results showed a similarly strong correlation between 1H-MRS and biochemistry (r=0.89, P = 0.0011).CONCLUSION1H-MRS can be carried out in ex vivo models, as well as in vivo, to detect and quantify intrahepatic fat content in the acute fatty liver. |
| first_indexed | 2024-03-12T02:10:07Z |
| format | Article |
| id | doaj.art-67147906dfd749969760e05139ad2425 |
| institution | Directory Open Access Journal |
| issn | 1305-3825 1305-3612 |
| language | English |
| last_indexed | 2024-03-12T02:10:07Z |
| publishDate | 2016-01-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Diagnostic and Interventional Radiology |
| spelling | doaj.art-67147906dfd749969760e05139ad24252023-09-06T13:42:23ZengGalenos Publishing HouseDiagnostic and Interventional Radiology1305-38251305-36122016-01-01221132110.5152/dir.2015.1433113049054In vivo and ex vivo measurements: noninvasive assessment of alcoholic fatty liver using 1H-MR spectroscopyDaniel Keese0Hüdayi Korkusuz1Frank Huebner2Dmitry Namgaladze3Bahram Raschidi4Thomas J. Vogl5 Department of Diagnostic and Interventional Radiology , Johannes Wolfgang Goethe University Frankfurt, Frankfurt, Germany; These authors contributed equally to this work Department of Diagnostic and Interventional Radiology , Johannes Wolfgang Goethe University Frankfurt, Frankfurt, Germany; These authors contributed equally to this work Department of Diagnostic and Interventional Radiology , Johannes Wolfgang Goethe University Frankfurt, Frankfurt, Germany Department of Biochemistry, Johannes Wolfgang Goethe University Frankfurt, Frankfurt, Germany Department of Diagnostic and Interventional Radiology , Johannes Wolfgang Goethe University Frankfurt, Frankfurt, Germany Department of Diagnostic and Interventional Radiology , Johannes Wolfgang Goethe University Frankfurt, Frankfurt, Germany PURPOSEWe aimed to evaluate the ability of 1H-magnetic resonance spectroscopy (1H-MRS) to detect and quantify hepatic fat content in vivo and ex vivo in an experimental rat model of alcoholic fatty liver using histopathology, biochemistry, and laboratory analyses as reference.METHODSAlcoholic fatty liver was induced within 48 hours in 20 Lewis rats; 10 rats served as control. Intrahepatic fat content determined by 1H-MRS was expressed as the percent ratio of the lipid and water peaks and was correlated with intrahepatic fat content determined histologically and biochemically. Liver enzymes were measured in serum.RESULTSFatty liver could be detected in vivo as well as ex vivo using 1H-MRS, in all 20 animals. Histologic analysis showed a fatty liver in 16 of 20 animals. Histology and 1H-MRS results were highly correlated (in vivo, r=0.93, P = 0.0005; ex vivo, r=0.92, P = 0.0006). Also a strong correlation was noted between in vivo 1H-MRS measurements and the fat content determined biochemically (r=0.96, P = 0.0003). Ex vivo results showed a similarly strong correlation between 1H-MRS and biochemistry (r=0.89, P = 0.0011).CONCLUSION1H-MRS can be carried out in ex vivo models, as well as in vivo, to detect and quantify intrahepatic fat content in the acute fatty liver. http://www.dirjournal.org/archives/archive-detail/article-preview/n-vivo-and-ex-vivo-measurements-noninvasive-assess/55531 |
| spellingShingle | Daniel Keese Hüdayi Korkusuz Frank Huebner Dmitry Namgaladze Bahram Raschidi Thomas J. Vogl In vivo and ex vivo measurements: noninvasive assessment of alcoholic fatty liver using 1H-MR spectroscopy Diagnostic and Interventional Radiology |
| title | In vivo and ex vivo measurements: noninvasive assessment of alcoholic fatty liver using 1H-MR spectroscopy |
| title_full | In vivo and ex vivo measurements: noninvasive assessment of alcoholic fatty liver using 1H-MR spectroscopy |
| title_fullStr | In vivo and ex vivo measurements: noninvasive assessment of alcoholic fatty liver using 1H-MR spectroscopy |
| title_full_unstemmed | In vivo and ex vivo measurements: noninvasive assessment of alcoholic fatty liver using 1H-MR spectroscopy |
| title_short | In vivo and ex vivo measurements: noninvasive assessment of alcoholic fatty liver using 1H-MR spectroscopy |
| title_sort | in vivo and ex vivo measurements noninvasive assessment of alcoholic fatty liver using 1h mr spectroscopy |
| url |
http://www.dirjournal.org/archives/archive-detail/article-preview/n-vivo-and-ex-vivo-measurements-noninvasive-assess/55531
|
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