Overexpression of Bacillus circulans alkaline protease in Bacillus subtilis and its potential application for recovery of protein from soybean dregs
Proteases are important for decomposition of proteins to generate peptides or amino acids and have a broad range of applications in different industries. Herein, a gene encoding an alkaline protease (AprBcp) from Bacillus circulans R1 was cloned and bioinformatics analyzed. In addition, a series of...
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| Format: | Article |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.968439/full |
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| author | Hao Chen Hao Chen Hao Chen Hao Chen Jie Wu Jie Wu Xiaodan Huang Xiaodan Huang Xuzhong Feng Hongwu Ji Hongwu Ji Liangzhong Zhao Liangzhong Zhao Jianrong Wang Jianrong Wang Jianrong Wang |
| author_facet | Hao Chen Hao Chen Hao Chen Hao Chen Jie Wu Jie Wu Xiaodan Huang Xiaodan Huang Xuzhong Feng Hongwu Ji Hongwu Ji Liangzhong Zhao Liangzhong Zhao Jianrong Wang Jianrong Wang Jianrong Wang |
| author_sort | Hao Chen |
| collection | DOAJ |
| description | Proteases are important for decomposition of proteins to generate peptides or amino acids and have a broad range of applications in different industries. Herein, a gene encoding an alkaline protease (AprBcp) from Bacillus circulans R1 was cloned and bioinformatics analyzed. In addition, a series of strategies were applied to achieve high-level expression of AprBcp in Bacillus subtilis. The maximum activity of AprBcp reached 165,870 U/ml after 60 h fed-batch cultivation in 50 l bioreactor. The purified recombinant AprBcp exhibited maximum activity at 60°C and pH 10.0, and remained stable in the range from pH 8.0 to 11.0 and 30 to 45°C. Metal ions Ca2+, Mn2+, and Mg2+ could improve the stability of AprBcp. Furthermore, the recombinant AprBcp displayed great potential application on the recovery of protein from soybean dregs. The results of this study will provide an effective method to prepare AprBcp in B. subtilis and its potential application on utilization of soybean dregs. |
| first_indexed | 2024-12-10T18:24:44Z |
| format | Article |
| id | doaj.art-67161f8f8c834548bd1c121e5d603ed2 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T18:24:44Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-67161f8f8c834548bd1c121e5d603ed22022-12-22T01:38:06ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-08-011310.3389/fmicb.2022.968439968439Overexpression of Bacillus circulans alkaline protease in Bacillus subtilis and its potential application for recovery of protein from soybean dregsHao Chen0Hao Chen1Hao Chen2Hao Chen3Jie Wu4Jie Wu5Xiaodan Huang6Xiaodan Huang7Xuzhong Feng8Hongwu Ji9Hongwu Ji10Liangzhong Zhao11Liangzhong Zhao12Jianrong Wang13Jianrong Wang14Jianrong Wang15College of Food and Chemical Engineering, Shaoyang University, Shaoyang, ChinaHunan Provincial Key Laboratory of Soybean Products Processing and Safety Control, Shaoyang, ChinaCollege of Food Science and Technology, Guangdong Ocean University, Zhanjiang, ChinaGuangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang, ChinaCollege of Food and Chemical Engineering, Shaoyang University, Shaoyang, ChinaHunan Provincial Key Laboratory of Soybean Products Processing and Safety Control, Shaoyang, ChinaCollege of Food Science and Technology, Guangdong Ocean University, Zhanjiang, ChinaGuangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang, ChinaShenzhen Shanggutang Food Development Co., Ltd.,Shenzhen, ChinaCollege of Food Science and Technology, Guangdong Ocean University, Zhanjiang, ChinaGuangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang, ChinaCollege of Food and Chemical Engineering, Shaoyang University, Shaoyang, ChinaHunan Provincial Key Laboratory of Soybean Products Processing and Safety Control, Shaoyang, ChinaCollege of Food and Chemical Engineering, Shaoyang University, Shaoyang, ChinaHunan Provincial Key Laboratory of Soybean Products Processing and Safety Control, Shaoyang, ChinaShenzhen Raink Ecology and Environment Co., Ltd.,Shenzhen, ChinaProteases are important for decomposition of proteins to generate peptides or amino acids and have a broad range of applications in different industries. Herein, a gene encoding an alkaline protease (AprBcp) from Bacillus circulans R1 was cloned and bioinformatics analyzed. In addition, a series of strategies were applied to achieve high-level expression of AprBcp in Bacillus subtilis. The maximum activity of AprBcp reached 165,870 U/ml after 60 h fed-batch cultivation in 50 l bioreactor. The purified recombinant AprBcp exhibited maximum activity at 60°C and pH 10.0, and remained stable in the range from pH 8.0 to 11.0 and 30 to 45°C. Metal ions Ca2+, Mn2+, and Mg2+ could improve the stability of AprBcp. Furthermore, the recombinant AprBcp displayed great potential application on the recovery of protein from soybean dregs. The results of this study will provide an effective method to prepare AprBcp in B. subtilis and its potential application on utilization of soybean dregs.https://www.frontiersin.org/articles/10.3389/fmicb.2022.968439/fullalkaline proteaseoverexpressionBacillus circulansBacillus subtilissoybean dregs |
| spellingShingle | Hao Chen Hao Chen Hao Chen Hao Chen Jie Wu Jie Wu Xiaodan Huang Xiaodan Huang Xuzhong Feng Hongwu Ji Hongwu Ji Liangzhong Zhao Liangzhong Zhao Jianrong Wang Jianrong Wang Jianrong Wang Overexpression of Bacillus circulans alkaline protease in Bacillus subtilis and its potential application for recovery of protein from soybean dregs Frontiers in Microbiology alkaline protease overexpression Bacillus circulans Bacillus subtilis soybean dregs |
| title | Overexpression of Bacillus circulans alkaline protease in Bacillus subtilis and its potential application for recovery of protein from soybean dregs |
| title_full | Overexpression of Bacillus circulans alkaline protease in Bacillus subtilis and its potential application for recovery of protein from soybean dregs |
| title_fullStr | Overexpression of Bacillus circulans alkaline protease in Bacillus subtilis and its potential application for recovery of protein from soybean dregs |
| title_full_unstemmed | Overexpression of Bacillus circulans alkaline protease in Bacillus subtilis and its potential application for recovery of protein from soybean dregs |
| title_short | Overexpression of Bacillus circulans alkaline protease in Bacillus subtilis and its potential application for recovery of protein from soybean dregs |
| title_sort | overexpression of bacillus circulans alkaline protease in bacillus subtilis and its potential application for recovery of protein from soybean dregs |
| topic | alkaline protease overexpression Bacillus circulans Bacillus subtilis soybean dregs |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.968439/full |
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