Salivary androgens in adolescence and their value as a marker of puberty: results from the SCAMP cohort
Context: Salivary androgens represent non-invasive biomarkers of puberty that may have utility in clinical and population studies. Objective: To understand normal age-related variation in salivary sex steroids and demonstrate their correlation to pubertal development in young adolescents. Design...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Bioscientifica
2023-11-01
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| Series: | Endocrine Connections |
| Subjects: | |
| Online Access: | https://ec.bioscientifica.com/view/journals/ec/12/12/EC-23-0084.xml |
| _version_ | 1827632497796579328 |
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| author | Supitcha Patjamontri Alexander Spiers Rachel B Smith Chen Shen Jo Adaway Brian G Keevil Mireille B Toledano S Faisal Ahmed |
| author_facet | Supitcha Patjamontri Alexander Spiers Rachel B Smith Chen Shen Jo Adaway Brian G Keevil Mireille B Toledano S Faisal Ahmed |
| author_sort | Supitcha Patjamontri |
| collection | DOAJ |
| description | Context: Salivary androgens represent non-invasive biomarkers of puberty that may have utility in clinical and population studies.
Objective: To understand normal age-related variation in salivary sex steroids and demonstrate their correlation to pubertal development in young adolescents.
Design, setting and participants: School-based cohort study of 1495 adolescents at two time points for collecting saliva samples approximately 2 years apart.
Outcome measures: The saliva samples were analyzed for five androgens (testostero ne, androstenedione (A4), 17-hydroxyprogesterone, 11-ketotestosterone and 11β-hydroxyandrostenedione) using liquid chromatography-mass spectrometry; in addition, salivary dehydroepiandrosterone (DHEA) and oestradiol (OE2) were analysed by ELISA. The pubertal staging was self-reported using the Pubertal Development Scale (PDS).
Results: In 1236 saliva samples from 903 boys aged between 11 and 16 years, salivary androgens except DHEA exhibited an increasing trend with an advancing age (ANOVA, P < 0.001), with salivary testosterone and A4 concentration showing the strongest correlation (r = 0.55, P < 0.001 and r = 0.48, P < 0.001, respectively). In a subgroup analysis of 155 and 63 saliva samples in boys and girls, respectively, morning salivary testosterone concentrations showed the highest correlation with composite PDS scores and voice-breaking category from PDS self-report in boys (r = 0.75, r = 0.67, respectively). In girls, salivary DHEA and OE2 had negligible correlations with age or composite PDS scores.
Conclusion: In boys aged 11–16 years, an increase in salivary testosterone and A4 is associated with self-reported pubertal progress and represents valid non-invasive biomarkers of puberty in boys. |
| first_indexed | 2024-03-09T14:45:24Z |
| format | Article |
| id | doaj.art-67168f56f61e43748acada80ebcf7400 |
| institution | Directory Open Access Journal |
| issn | 2049-3614 |
| language | English |
| last_indexed | 2024-03-09T14:45:24Z |
| publishDate | 2023-11-01 |
| publisher | Bioscientifica |
| record_format | Article |
| series | Endocrine Connections |
| spelling | doaj.art-67168f56f61e43748acada80ebcf74002023-11-27T07:04:53ZengBioscientificaEndocrine Connections2049-36142023-11-011212114https://doi.org/10.1530/EC-23-0084Salivary androgens in adolescence and their value as a marker of puberty: results from the SCAMP cohortSupitcha Patjamontri0Alexander Spiers1Rachel B Smith2Chen Shen3Jo Adaway4Brian G Keevil5Mireille B Toledano6S Faisal Ahmed7Developmental Endocrinology Research Group, University of Glasgow, Royal Hospital for Children, Glasgow, UK; Division of Endocrinology and Metabolism, Department of Pediatrics, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand MRC Centre for Environment and Health, Imperial College London, London, UK; NIHR Health Protection Research Unit on Chemical Radiation Threats and Hazards, Imperial College London, London, UKMRC Centre for Environment and Health, Imperial College London, London, UK; NIHR Health Protection Research Unit on Chemical Radiation Threats and Hazards, Imperial College London, London, UK; National Institute for Health Research (NIHR) Health Protection Research Unit in Environmental Exposures and Health, Imperial College London, London, UK; Mohn Centre for Children’s Health and Wellbeing, Imperial College London, London, UK MRC Centre for Environment and Health, Imperial College London, London, UK; NIHR Health Protection Research Unit on Chemical Radiation Threats and Hazards, Imperial College London, London, UKDepartment of Clinical Biochemistry, Manchester University NHS Foundation Trust, Manchester Academic Health Science Centre, The University of Manchester, Manchester, UKDepartment of Clinical Biochemistry, Manchester University NHS Foundation Trust, Manchester Academic Health Science Centre, The University of Manchester, Manchester, UKMRC Centre for Environment and Health, Imperial College London, London, UK; NIHR Health Protection Research Unit on Chemical Radiation Threats and Hazards, Imperial College London, London, UK; National Institute for Health Research (NIHR) Health Protection Research Unit in Environmental Exposures and Health, Imperial College London, London, UK; Mohn Centre for Children’s Health and Wellbeing, Imperial College London, London, UK Developmental Endocrinology Research Group, University of Glasgow, Royal Hospital for Children, Glasgow, UKContext: Salivary androgens represent non-invasive biomarkers of puberty that may have utility in clinical and population studies. Objective: To understand normal age-related variation in salivary sex steroids and demonstrate their correlation to pubertal development in young adolescents. Design, setting and participants: School-based cohort study of 1495 adolescents at two time points for collecting saliva samples approximately 2 years apart. Outcome measures: The saliva samples were analyzed for five androgens (testostero ne, androstenedione (A4), 17-hydroxyprogesterone, 11-ketotestosterone and 11β-hydroxyandrostenedione) using liquid chromatography-mass spectrometry; in addition, salivary dehydroepiandrosterone (DHEA) and oestradiol (OE2) were analysed by ELISA. The pubertal staging was self-reported using the Pubertal Development Scale (PDS). Results: In 1236 saliva samples from 903 boys aged between 11 and 16 years, salivary androgens except DHEA exhibited an increasing trend with an advancing age (ANOVA, P < 0.001), with salivary testosterone and A4 concentration showing the strongest correlation (r = 0.55, P < 0.001 and r = 0.48, P < 0.001, respectively). In a subgroup analysis of 155 and 63 saliva samples in boys and girls, respectively, morning salivary testosterone concentrations showed the highest correlation with composite PDS scores and voice-breaking category from PDS self-report in boys (r = 0.75, r = 0.67, respectively). In girls, salivary DHEA and OE2 had negligible correlations with age or composite PDS scores. Conclusion: In boys aged 11–16 years, an increase in salivary testosterone and A4 is associated with self-reported pubertal progress and represents valid non-invasive biomarkers of puberty in boys.https://ec.bioscientifica.com/view/journals/ec/12/12/EC-23-0084.xmlandrostenedioneliquid chromatographymass spectrometry (lc-msms)pubertal development scale (pds)salivatestosterone |
| spellingShingle | Supitcha Patjamontri Alexander Spiers Rachel B Smith Chen Shen Jo Adaway Brian G Keevil Mireille B Toledano S Faisal Ahmed Salivary androgens in adolescence and their value as a marker of puberty: results from the SCAMP cohort Endocrine Connections androstenedione liquid chromatographymass spectrometry (lc-msms) pubertal development scale (pds) saliva testosterone |
| title | Salivary androgens in adolescence and their value as a marker of puberty: results from the SCAMP cohort |
| title_full | Salivary androgens in adolescence and their value as a marker of puberty: results from the SCAMP cohort |
| title_fullStr | Salivary androgens in adolescence and their value as a marker of puberty: results from the SCAMP cohort |
| title_full_unstemmed | Salivary androgens in adolescence and their value as a marker of puberty: results from the SCAMP cohort |
| title_short | Salivary androgens in adolescence and their value as a marker of puberty: results from the SCAMP cohort |
| title_sort | salivary androgens in adolescence and their value as a marker of puberty results from the scamp cohort |
| topic | androstenedione liquid chromatographymass spectrometry (lc-msms) pubertal development scale (pds) saliva testosterone |
| url | https://ec.bioscientifica.com/view/journals/ec/12/12/EC-23-0084.xml |
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