Deep Eutectic Solvents as New Extraction Media for Flavonoids in Mung Bean

Mung beans contain abundant flavonoids like vitexin and isovitexin, which contribute to their strong bioactivities, such as antioxidant effects, so efforts should focus on extracting bioactive flavonoids as well as aligning with the goal of green extraction for specific applications. Deep eutectic s...

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Main Authors: Jingyu Gao, Longli Xie, Yu Peng, Mo Li, Jingming Li, Yuanying Ni, Xin Wen
Format: Article
Language:English
Published: MDPI AG 2024-03-01
Series:Foods
Subjects:
Online Access:https://www.mdpi.com/2304-8158/13/5/777
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author Jingyu Gao
Longli Xie
Yu Peng
Mo Li
Jingming Li
Yuanying Ni
Xin Wen
author_facet Jingyu Gao
Longli Xie
Yu Peng
Mo Li
Jingming Li
Yuanying Ni
Xin Wen
author_sort Jingyu Gao
collection DOAJ
description Mung beans contain abundant flavonoids like vitexin and isovitexin, which contribute to their strong bioactivities, such as antioxidant effects, so efforts should focus on extracting bioactive flavonoids as well as aligning with the goal of green extraction for specific applications. Deep eutectic solvent coupled with ultrasound-assisted extraction (DES-UAE) was applied to extract flavonoids from mung beans, and eight different DESs were compared on the extraction yield. In addition, the traditional extraction method with 30% ethanol was performed as the reference. The results showed that ethylene glycol-glycolic acid achieved the highest yield among all the DESs, 1.6 times that of the reference values. Furthermore, the DES-UAE parameters were optimized as a 60 mL/g liquid–solid ratio, 30% water content in DES, 200 W ultrasonic power, 67 °C ultrasonic temperature, and 10 min extraction time, leading to the DES extract with the maximum extraction yield of 2339.45 ± 42.98 μg/g, and the significantly stronger DPPH and ABTS radical scavenging ability than the traditional extract. Therefore, employing DES and ultrasonic extraction together offers a green method for extracting flavonoids from mung beans, advancing the development and utilization of plant-derived effective components in a sustainable manner.
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spelling doaj.art-6719fc546a5146f1ac812ed60c8df3a12024-03-12T16:44:27ZengMDPI AGFoods2304-81582024-03-0113577710.3390/foods13050777Deep Eutectic Solvents as New Extraction Media for Flavonoids in Mung BeanJingyu Gao0Longli Xie1Yu Peng2Mo Li3Jingming Li4Yuanying Ni5Xin Wen6College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaMung beans contain abundant flavonoids like vitexin and isovitexin, which contribute to their strong bioactivities, such as antioxidant effects, so efforts should focus on extracting bioactive flavonoids as well as aligning with the goal of green extraction for specific applications. Deep eutectic solvent coupled with ultrasound-assisted extraction (DES-UAE) was applied to extract flavonoids from mung beans, and eight different DESs were compared on the extraction yield. In addition, the traditional extraction method with 30% ethanol was performed as the reference. The results showed that ethylene glycol-glycolic acid achieved the highest yield among all the DESs, 1.6 times that of the reference values. Furthermore, the DES-UAE parameters were optimized as a 60 mL/g liquid–solid ratio, 30% water content in DES, 200 W ultrasonic power, 67 °C ultrasonic temperature, and 10 min extraction time, leading to the DES extract with the maximum extraction yield of 2339.45 ± 42.98 μg/g, and the significantly stronger DPPH and ABTS radical scavenging ability than the traditional extract. Therefore, employing DES and ultrasonic extraction together offers a green method for extracting flavonoids from mung beans, advancing the development and utilization of plant-derived effective components in a sustainable manner.https://www.mdpi.com/2304-8158/13/5/777mung beanflavonoidsdeep eutectic solventultrasound-assisted extractionantioxidant capacity
spellingShingle Jingyu Gao
Longli Xie
Yu Peng
Mo Li
Jingming Li
Yuanying Ni
Xin Wen
Deep Eutectic Solvents as New Extraction Media for Flavonoids in Mung Bean
Foods
mung bean
flavonoids
deep eutectic solvent
ultrasound-assisted extraction
antioxidant capacity
title Deep Eutectic Solvents as New Extraction Media for Flavonoids in Mung Bean
title_full Deep Eutectic Solvents as New Extraction Media for Flavonoids in Mung Bean
title_fullStr Deep Eutectic Solvents as New Extraction Media for Flavonoids in Mung Bean
title_full_unstemmed Deep Eutectic Solvents as New Extraction Media for Flavonoids in Mung Bean
title_short Deep Eutectic Solvents as New Extraction Media for Flavonoids in Mung Bean
title_sort deep eutectic solvents as new extraction media for flavonoids in mung bean
topic mung bean
flavonoids
deep eutectic solvent
ultrasound-assisted extraction
antioxidant capacity
url https://www.mdpi.com/2304-8158/13/5/777
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