Effects of Argon Gas Plasma Treatment on Biocompatibility of Nanostructured Titanium
In this study, we applied argon plasma treatment to titanium surfaces with nanostructures deposited by concentrated alkali treatment and investigated the effects on the surface of the material and the tissue surrounding an implant site. The results showed that the treatment with argon plasma removed...
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| Language: | English |
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MDPI AG
2023-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/1/149 |
| _version_ | 1797358729715253248 |
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| author | Rina Hayashi Seiji Takao Satoshi Komasa Tohru Sekino Tetsuji Kusumoto Kenji Maekawa |
| author_facet | Rina Hayashi Seiji Takao Satoshi Komasa Tohru Sekino Tetsuji Kusumoto Kenji Maekawa |
| author_sort | Rina Hayashi |
| collection | DOAJ |
| description | In this study, we applied argon plasma treatment to titanium surfaces with nanostructures deposited by concentrated alkali treatment and investigated the effects on the surface of the material and the tissue surrounding an implant site. The results showed that the treatment with argon plasma removed carbon contaminants and increased the surface energy of the material while the nanoscale network structure deposited on the titanium surface remained in place. Reactive oxygen species reduced the oxidative stress of bone marrow cells on the treated titanium surface, creating a favorable environment for cell proliferation. Good results were observed in vitro evaluations using rat bone marrow cells. The group treated with argon plasma exhibited the highest apatite formation in experiments using simulated body fluids. The results of in vivo evaluation using rat femurs revealed that the treatment improved the amount of new bone formation around an implant. Thus, the results demonstrate that argon plasma treatment enhances the ability of nanostructured titanium surfaces to induce hard tissue differentiation and supports new bone formation around an implant site. |
| first_indexed | 2024-03-08T15:06:21Z |
| format | Article |
| id | doaj.art-671e1b4a7ce843a4aa1b43c52bfea0f9 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T15:06:21Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-671e1b4a7ce843a4aa1b43c52bfea0f92024-01-10T14:58:20ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0125114910.3390/ijms25010149Effects of Argon Gas Plasma Treatment on Biocompatibility of Nanostructured TitaniumRina Hayashi0Seiji Takao1Satoshi Komasa2Tohru Sekino3Tetsuji Kusumoto4Kenji Maekawa5Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata 573-1121, Osaka, JapanDepartment of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata 573-1121, Osaka, JapanDepartment of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata 573-1121, Osaka, JapanDepartment of Advanced Hard Materials, SANKEN (The Institute of Scientific and Industrial Research), Osaka University, 8-1 Mihogaoka, Ibaraki 567-0047, Osaka, JapanDepartment of Oral Health Engineering, Faculty of Health Sciences, Osaka Dental University, 1-4-4, Makino-honmachi, Hirakata 573-1121, Osaka, JapanDepartment of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata 573-1121, Osaka, JapanIn this study, we applied argon plasma treatment to titanium surfaces with nanostructures deposited by concentrated alkali treatment and investigated the effects on the surface of the material and the tissue surrounding an implant site. The results showed that the treatment with argon plasma removed carbon contaminants and increased the surface energy of the material while the nanoscale network structure deposited on the titanium surface remained in place. Reactive oxygen species reduced the oxidative stress of bone marrow cells on the treated titanium surface, creating a favorable environment for cell proliferation. Good results were observed in vitro evaluations using rat bone marrow cells. The group treated with argon plasma exhibited the highest apatite formation in experiments using simulated body fluids. The results of in vivo evaluation using rat femurs revealed that the treatment improved the amount of new bone formation around an implant. Thus, the results demonstrate that argon plasma treatment enhances the ability of nanostructured titanium surfaces to induce hard tissue differentiation and supports new bone formation around an implant site.https://www.mdpi.com/1422-0067/25/1/149argon plasma treatmenttitania nanosheetsreactive oxygen specieshard tissue differentiationrat bone marrowbone formation |
| spellingShingle | Rina Hayashi Seiji Takao Satoshi Komasa Tohru Sekino Tetsuji Kusumoto Kenji Maekawa Effects of Argon Gas Plasma Treatment on Biocompatibility of Nanostructured Titanium International Journal of Molecular Sciences argon plasma treatment titania nanosheets reactive oxygen species hard tissue differentiation rat bone marrow bone formation |
| title | Effects of Argon Gas Plasma Treatment on Biocompatibility of Nanostructured Titanium |
| title_full | Effects of Argon Gas Plasma Treatment on Biocompatibility of Nanostructured Titanium |
| title_fullStr | Effects of Argon Gas Plasma Treatment on Biocompatibility of Nanostructured Titanium |
| title_full_unstemmed | Effects of Argon Gas Plasma Treatment on Biocompatibility of Nanostructured Titanium |
| title_short | Effects of Argon Gas Plasma Treatment on Biocompatibility of Nanostructured Titanium |
| title_sort | effects of argon gas plasma treatment on biocompatibility of nanostructured titanium |
| topic | argon plasma treatment titania nanosheets reactive oxygen species hard tissue differentiation rat bone marrow bone formation |
| url | https://www.mdpi.com/1422-0067/25/1/149 |
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