Lectin histochemistry of Kudoa septempunctata genotype ST3-infected muscle of olive flounder (Paralichthys olivaceus)

The localization of carbohydrate terminals in Kudoa septempunctata ST3-infected muscle of olive flounder (Paralichthys olivaceus) was investigated using lectin histochemistry to determine the types of carbohydrate sugar residues expressed in Kudoa spores. Twenty-one lectins were examined, i.e., N-ac...

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Main Authors: Kang Jaeyoun, Park Changnam, Jang Yeounghwan, Ahn Meejung, Shin Taekyun
Format: Article
Language:English
Published: EDP Sciences 2016-01-01
Series:Parasite
Subjects:
Online Access:http://dx.doi.org/10.1051/parasite/2016021
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author Kang Jaeyoun
Park Changnam
Jang Yeounghwan
Ahn Meejung
Shin Taekyun
author_facet Kang Jaeyoun
Park Changnam
Jang Yeounghwan
Ahn Meejung
Shin Taekyun
author_sort Kang Jaeyoun
collection DOAJ
description The localization of carbohydrate terminals in Kudoa septempunctata ST3-infected muscle of olive flounder (Paralichthys olivaceus) was investigated using lectin histochemistry to determine the types of carbohydrate sugar residues expressed in Kudoa spores. Twenty-one lectins were examined, i.e., N-acetylglucosamine (s-WGA, WGA, DSL-II, DSL, LEL, STL), mannose (Con A, LCA, PSA), galactose/N-acetylgalactosamine (RCA12, BSL-I, VVA, DBA, SBA, SJA, Jacalin, PNA, ECL), complex type N-glycans (PHA-E and PHA-L), and fucose (UEA-I). Spores encased by a plasmodial membrane were labeled for the majority of these lectins, with the exception of LCA, PSA, PNA, and PHA-L. Four lectins (RCA 120, BSL-I, DBA, and SJA) belonging to the galactose/N-acetylgalactosamine group, only labeled spores, but not the plasmodial membrane. This is the first confirmation that various sugar residues are present in spores and plasmodial membranes of K. septempunctata ST3.
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spelling doaj.art-671f11fbd4a3491e9e101cdaea3332b52023-12-02T15:30:35ZengEDP SciencesParasite1776-10422016-01-01232110.1051/parasite/2016021parasite160010Lectin histochemistry of Kudoa septempunctata genotype ST3-infected muscle of olive flounder (Paralichthys olivaceus)Kang JaeyounPark ChangnamJang YeounghwanAhn MeejungShin TaekyunThe localization of carbohydrate terminals in Kudoa septempunctata ST3-infected muscle of olive flounder (Paralichthys olivaceus) was investigated using lectin histochemistry to determine the types of carbohydrate sugar residues expressed in Kudoa spores. Twenty-one lectins were examined, i.e., N-acetylglucosamine (s-WGA, WGA, DSL-II, DSL, LEL, STL), mannose (Con A, LCA, PSA), galactose/N-acetylgalactosamine (RCA12, BSL-I, VVA, DBA, SBA, SJA, Jacalin, PNA, ECL), complex type N-glycans (PHA-E and PHA-L), and fucose (UEA-I). Spores encased by a plasmodial membrane were labeled for the majority of these lectins, with the exception of LCA, PSA, PNA, and PHA-L. Four lectins (RCA 120, BSL-I, DBA, and SJA) belonging to the galactose/N-acetylgalactosamine group, only labeled spores, but not the plasmodial membrane. This is the first confirmation that various sugar residues are present in spores and plasmodial membranes of K. septempunctata ST3.http://dx.doi.org/10.1051/parasite/2016021Kudoa septempunctataLectinMyxozoaOlive flounder
spellingShingle Kang Jaeyoun
Park Changnam
Jang Yeounghwan
Ahn Meejung
Shin Taekyun
Lectin histochemistry of Kudoa septempunctata genotype ST3-infected muscle of olive flounder (Paralichthys olivaceus)
Parasite
Kudoa septempunctata
Lectin
Myxozoa
Olive flounder
title Lectin histochemistry of Kudoa septempunctata genotype ST3-infected muscle of olive flounder (Paralichthys olivaceus)
title_full Lectin histochemistry of Kudoa septempunctata genotype ST3-infected muscle of olive flounder (Paralichthys olivaceus)
title_fullStr Lectin histochemistry of Kudoa septempunctata genotype ST3-infected muscle of olive flounder (Paralichthys olivaceus)
title_full_unstemmed Lectin histochemistry of Kudoa septempunctata genotype ST3-infected muscle of olive flounder (Paralichthys olivaceus)
title_short Lectin histochemistry of Kudoa septempunctata genotype ST3-infected muscle of olive flounder (Paralichthys olivaceus)
title_sort lectin histochemistry of kudoa septempunctata genotype st3 infected muscle of olive flounder paralichthys olivaceus
topic Kudoa septempunctata
Lectin
Myxozoa
Olive flounder
url http://dx.doi.org/10.1051/parasite/2016021
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