Lectin histochemistry of Kudoa septempunctata genotype ST3-infected muscle of olive flounder (Paralichthys olivaceus)
The localization of carbohydrate terminals in Kudoa septempunctata ST3-infected muscle of olive flounder (Paralichthys olivaceus) was investigated using lectin histochemistry to determine the types of carbohydrate sugar residues expressed in Kudoa spores. Twenty-one lectins were examined, i.e., N-ac...
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EDP Sciences
2016-01-01
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Series: | Parasite |
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Online Access: | http://dx.doi.org/10.1051/parasite/2016021 |
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author | Kang Jaeyoun Park Changnam Jang Yeounghwan Ahn Meejung Shin Taekyun |
author_facet | Kang Jaeyoun Park Changnam Jang Yeounghwan Ahn Meejung Shin Taekyun |
author_sort | Kang Jaeyoun |
collection | DOAJ |
description | The localization of carbohydrate terminals in Kudoa septempunctata ST3-infected muscle of olive flounder (Paralichthys olivaceus) was investigated using lectin histochemistry to determine the types of carbohydrate sugar residues expressed in Kudoa spores. Twenty-one lectins were examined, i.e., N-acetylglucosamine (s-WGA, WGA, DSL-II, DSL, LEL, STL), mannose (Con A, LCA, PSA), galactose/N-acetylgalactosamine (RCA12, BSL-I, VVA, DBA, SBA, SJA, Jacalin, PNA, ECL), complex type N-glycans (PHA-E and PHA-L), and fucose (UEA-I). Spores encased by a plasmodial membrane were labeled for the majority of these lectins, with the exception of LCA, PSA, PNA, and PHA-L. Four lectins (RCA 120, BSL-I, DBA, and SJA) belonging to the galactose/N-acetylgalactosamine group, only labeled spores, but not the plasmodial membrane. This is the first confirmation that various sugar residues are present in spores and plasmodial membranes of K. septempunctata ST3. |
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id | doaj.art-671f11fbd4a3491e9e101cdaea3332b5 |
institution | Directory Open Access Journal |
issn | 1776-1042 |
language | English |
last_indexed | 2024-03-09T08:45:49Z |
publishDate | 2016-01-01 |
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series | Parasite |
spelling | doaj.art-671f11fbd4a3491e9e101cdaea3332b52023-12-02T15:30:35ZengEDP SciencesParasite1776-10422016-01-01232110.1051/parasite/2016021parasite160010Lectin histochemistry of Kudoa septempunctata genotype ST3-infected muscle of olive flounder (Paralichthys olivaceus)Kang JaeyounPark ChangnamJang YeounghwanAhn MeejungShin TaekyunThe localization of carbohydrate terminals in Kudoa septempunctata ST3-infected muscle of olive flounder (Paralichthys olivaceus) was investigated using lectin histochemistry to determine the types of carbohydrate sugar residues expressed in Kudoa spores. Twenty-one lectins were examined, i.e., N-acetylglucosamine (s-WGA, WGA, DSL-II, DSL, LEL, STL), mannose (Con A, LCA, PSA), galactose/N-acetylgalactosamine (RCA12, BSL-I, VVA, DBA, SBA, SJA, Jacalin, PNA, ECL), complex type N-glycans (PHA-E and PHA-L), and fucose (UEA-I). Spores encased by a plasmodial membrane were labeled for the majority of these lectins, with the exception of LCA, PSA, PNA, and PHA-L. Four lectins (RCA 120, BSL-I, DBA, and SJA) belonging to the galactose/N-acetylgalactosamine group, only labeled spores, but not the plasmodial membrane. This is the first confirmation that various sugar residues are present in spores and plasmodial membranes of K. septempunctata ST3.http://dx.doi.org/10.1051/parasite/2016021Kudoa septempunctataLectinMyxozoaOlive flounder |
spellingShingle | Kang Jaeyoun Park Changnam Jang Yeounghwan Ahn Meejung Shin Taekyun Lectin histochemistry of Kudoa septempunctata genotype ST3-infected muscle of olive flounder (Paralichthys olivaceus) Parasite Kudoa septempunctata Lectin Myxozoa Olive flounder |
title | Lectin histochemistry of Kudoa septempunctata genotype ST3-infected muscle of olive flounder (Paralichthys olivaceus) |
title_full | Lectin histochemistry of Kudoa septempunctata genotype ST3-infected muscle of olive flounder (Paralichthys olivaceus) |
title_fullStr | Lectin histochemistry of Kudoa septempunctata genotype ST3-infected muscle of olive flounder (Paralichthys olivaceus) |
title_full_unstemmed | Lectin histochemistry of Kudoa septempunctata genotype ST3-infected muscle of olive flounder (Paralichthys olivaceus) |
title_short | Lectin histochemistry of Kudoa septempunctata genotype ST3-infected muscle of olive flounder (Paralichthys olivaceus) |
title_sort | lectin histochemistry of kudoa septempunctata genotype st3 infected muscle of olive flounder paralichthys olivaceus |
topic | Kudoa septempunctata Lectin Myxozoa Olive flounder |
url | http://dx.doi.org/10.1051/parasite/2016021 |
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