Antibacterial and Antibiofilm Activity of Different Species of <i>Fabiana</i> sp. Extract Obtained via Maceration and Ultrasound-Assisted Extraction against <i>Staphylococcus epidermidis</i>
<i>Staphylococcus epidermidis</i> is an opportunistic pathogen that, under certain conditions, can induce aggravated infectious processes, mainly in immunosuppressed patients. Moreover, <i>S. epidermidis</i> is one of the leading causes of medical device- and implant-associat...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/12/9/1830 |
| _version_ | 1797601923516334080 |
|---|---|
| author | José Martínez Chamás María Inés Isla Iris Catiana Zampini |
| author_facet | José Martínez Chamás María Inés Isla Iris Catiana Zampini |
| author_sort | José Martínez Chamás |
| collection | DOAJ |
| description | <i>Staphylococcus epidermidis</i> is an opportunistic pathogen that, under certain conditions, can induce aggravated infectious processes, mainly in immunosuppressed patients. Moreover, <i>S. epidermidis</i> is one of the leading causes of medical device- and implant-associated infections and is also recognized as a canonical biofilm producer. <i>Fabiana punensis</i>, <i>F. densa</i> and <i>F. patagonica</i> are three medicinal plants that grow in arid environments in Argentina (Altoandina, Puna, Prepuna and Monte regions). In this work, we studied the antimicrobial activity of alcoholic extracts of these plant species obtained via maceration (M) and ultrasound-assisted extraction (UAE) against <i>S. epidermidis</i>. In addition, the antibiofilm activity of the <i>F. densa</i> extract was also evaluated. It was found that the extracts obtained via M did not present differences with those obtained via UAE regarding the chemical profile. <i>F. densa</i> showed the lowest minimum inhibitory concentration (MIC) value (75 µg GAE/mL). At concentrations higher than the MIC, the extract induced the release of cellular constituents. At the concentration of 1/8× MIC, the extract inhibited biofilm formation by 78%, reducing metabolic activity by 67%. On the other hand, it presented a low percentage of preformed biofilm removal. In all assays, gallic acid (GA) has been used as a reference antimicrobial compound. Finally, it was shown via microscopy visualization that the extract reduces adhesion to hydrophobic and hydrophilic surfaces. Thus, <i>F. densa</i> extracts could potentially be used for the antibiotic treatment of infections produced by <i>S. epidermidis</i> or as an inhibitor agent of production biofilm, avoiding infections caused by medical devices. |
| first_indexed | 2024-03-11T04:10:04Z |
| format | Article |
| id | doaj.art-67226d4712824c3fb3aa6271ed379380 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T04:10:04Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-67226d4712824c3fb3aa6271ed3793802023-11-17T23:32:18ZengMDPI AGPlants2223-77472023-04-01129183010.3390/plants12091830Antibacterial and Antibiofilm Activity of Different Species of <i>Fabiana</i> sp. Extract Obtained via Maceration and Ultrasound-Assisted Extraction against <i>Staphylococcus epidermidis</i>José Martínez Chamás0María Inés Isla1Iris Catiana Zampini2Laboratorio de Investigación de Productos Naturales (LIPRON), Instituto de Bioprospección y Fisiología Vegetal (INBIOFIV-CONICET-UNT), Facultad de Ciencias Naturales e IML, Universidad Nacional de Tucumán, San Lorenzo 1469, San Miguel de Tucumán PC:4000, Tucumán, ArgentinaLaboratorio de Investigación de Productos Naturales (LIPRON), Instituto de Bioprospección y Fisiología Vegetal (INBIOFIV-CONICET-UNT), Facultad de Ciencias Naturales e IML, Universidad Nacional de Tucumán, San Lorenzo 1469, San Miguel de Tucumán PC:4000, Tucumán, ArgentinaLaboratorio de Investigación de Productos Naturales (LIPRON), Instituto de Bioprospección y Fisiología Vegetal (INBIOFIV-CONICET-UNT), Facultad de Ciencias Naturales e IML, Universidad Nacional de Tucumán, San Lorenzo 1469, San Miguel de Tucumán PC:4000, Tucumán, Argentina<i>Staphylococcus epidermidis</i> is an opportunistic pathogen that, under certain conditions, can induce aggravated infectious processes, mainly in immunosuppressed patients. Moreover, <i>S. epidermidis</i> is one of the leading causes of medical device- and implant-associated infections and is also recognized as a canonical biofilm producer. <i>Fabiana punensis</i>, <i>F. densa</i> and <i>F. patagonica</i> are three medicinal plants that grow in arid environments in Argentina (Altoandina, Puna, Prepuna and Monte regions). In this work, we studied the antimicrobial activity of alcoholic extracts of these plant species obtained via maceration (M) and ultrasound-assisted extraction (UAE) against <i>S. epidermidis</i>. In addition, the antibiofilm activity of the <i>F. densa</i> extract was also evaluated. It was found that the extracts obtained via M did not present differences with those obtained via UAE regarding the chemical profile. <i>F. densa</i> showed the lowest minimum inhibitory concentration (MIC) value (75 µg GAE/mL). At concentrations higher than the MIC, the extract induced the release of cellular constituents. At the concentration of 1/8× MIC, the extract inhibited biofilm formation by 78%, reducing metabolic activity by 67%. On the other hand, it presented a low percentage of preformed biofilm removal. In all assays, gallic acid (GA) has been used as a reference antimicrobial compound. Finally, it was shown via microscopy visualization that the extract reduces adhesion to hydrophobic and hydrophilic surfaces. Thus, <i>F. densa</i> extracts could potentially be used for the antibiotic treatment of infections produced by <i>S. epidermidis</i> or as an inhibitor agent of production biofilm, avoiding infections caused by medical devices.https://www.mdpi.com/2223-7747/12/9/1830<i>Fabiana</i> species<i>Fabiana densa</i><i>Staphylococcus epidermidis</i>ultrasound-assisted extraction antibacterialantibiofilm |
| spellingShingle | José Martínez Chamás María Inés Isla Iris Catiana Zampini Antibacterial and Antibiofilm Activity of Different Species of <i>Fabiana</i> sp. Extract Obtained via Maceration and Ultrasound-Assisted Extraction against <i>Staphylococcus epidermidis</i> Plants <i>Fabiana</i> species <i>Fabiana densa</i> <i>Staphylococcus epidermidis</i> ultrasound-assisted extraction antibacterial antibiofilm |
| title | Antibacterial and Antibiofilm Activity of Different Species of <i>Fabiana</i> sp. Extract Obtained via Maceration and Ultrasound-Assisted Extraction against <i>Staphylococcus epidermidis</i> |
| title_full | Antibacterial and Antibiofilm Activity of Different Species of <i>Fabiana</i> sp. Extract Obtained via Maceration and Ultrasound-Assisted Extraction against <i>Staphylococcus epidermidis</i> |
| title_fullStr | Antibacterial and Antibiofilm Activity of Different Species of <i>Fabiana</i> sp. Extract Obtained via Maceration and Ultrasound-Assisted Extraction against <i>Staphylococcus epidermidis</i> |
| title_full_unstemmed | Antibacterial and Antibiofilm Activity of Different Species of <i>Fabiana</i> sp. Extract Obtained via Maceration and Ultrasound-Assisted Extraction against <i>Staphylococcus epidermidis</i> |
| title_short | Antibacterial and Antibiofilm Activity of Different Species of <i>Fabiana</i> sp. Extract Obtained via Maceration and Ultrasound-Assisted Extraction against <i>Staphylococcus epidermidis</i> |
| title_sort | antibacterial and antibiofilm activity of different species of i fabiana i sp extract obtained via maceration and ultrasound assisted extraction against i staphylococcus epidermidis i |
| topic | <i>Fabiana</i> species <i>Fabiana densa</i> <i>Staphylococcus epidermidis</i> ultrasound-assisted extraction antibacterial antibiofilm |
| url | https://www.mdpi.com/2223-7747/12/9/1830 |
| work_keys_str_mv | AT josemartinezchamas antibacterialandantibiofilmactivityofdifferentspeciesofifabianaispextractobtainedviamacerationandultrasoundassistedextractionagainstistaphylococcusepidermidisi AT mariainesisla antibacterialandantibiofilmactivityofdifferentspeciesofifabianaispextractobtainedviamacerationandultrasoundassistedextractionagainstistaphylococcusepidermidisi AT iriscatianazampini antibacterialandantibiofilmactivityofdifferentspeciesofifabianaispextractobtainedviamacerationandultrasoundassistedextractionagainstistaphylococcusepidermidisi |