The Effect of Bone Marrow Mesenchymal Stem Cells on the Granulocytic Differentiation of HL-60 Cells
Objective: Mesenchymal stem cells (MSCs) are multipotent stromal cells that can differentiate into a variety of cell types. They control the process of hematopoiesis by secreting regulatory cytokines and growth factors and by the expression of important cell adhesion molecules for cell-to-cell inter...
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2018-02-01
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| Series: | Turkish Journal of Hematology |
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| Online Access: | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-38039 |
| _version_ | 1797911119450341376 |
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| author | Hossein Nikkhah Elham Safarzadeh Karim Shamsasenjan Mehdi Yousefi Parisa Lotfinejad Mehdi Talebi Mozhde Mohammadian Farhoud Golafshan Ali Akbar Movassaghpour |
| author_facet | Hossein Nikkhah Elham Safarzadeh Karim Shamsasenjan Mehdi Yousefi Parisa Lotfinejad Mehdi Talebi Mozhde Mohammadian Farhoud Golafshan Ali Akbar Movassaghpour |
| author_sort | Hossein Nikkhah |
| collection | DOAJ |
| description | Objective: Mesenchymal stem cells (MSCs) are multipotent stromal cells that can differentiate into a variety of cell types. They control the process of hematopoiesis by secreting regulatory cytokines and growth factors and by the expression of important cell adhesion molecules for cell-to-cell interactions. This investigation was intended to examine the effect of bone marrow (BM)-derived MSCs on the differentiation of HL-60 cells according to morphological evaluation, flow cytometry analysis, and gene expression profile. Materials and Methods: The BM-MSCs were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (FBS). After the third passage, the BM-MSCs were irradiated at 30 Gy. To compare how the HL-60 cells differentiated in groups treated differently, HL-60 cells were cultured in RPMI-1640 and supplemented with 10% FBS. The HL-60 cells were seeded into six-well culture plates and treated with all-trans-retinoic acid (ATRA), BM-MSCs, or BM-MSCs in combination with ATRA, while one well remained as untreated HL-60 cells. The expression levels of the granulocyte subsetspecific genes in the HL-60 cells were assayed by real-time polymerase chain reaction. Results: Our results revealed that BM-MSCs support the granulocytic differentiation of the human promyelocytic leukemia cell line HL-60. Conclusion: Based on the results of this study, we concluded that BM-MSCs may be an effective resource in reducing or even preventing ATRA's side effects and may promote differentiation for short medication periods. Though BM-MSCs are effective resources, more complementary studies are necessary to improve this differentiation mechanism in clinical cases. |
| first_indexed | 2024-04-10T11:35:21Z |
| format | Article |
| id | doaj.art-6725b04e3b104330ae154857dafb03dd |
| institution | Directory Open Access Journal |
| issn | 1308-5263 |
| language | English |
| last_indexed | 2024-04-10T11:35:21Z |
| publishDate | 2018-02-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Turkish Journal of Hematology |
| spelling | doaj.art-6725b04e3b104330ae154857dafb03dd2023-02-15T16:17:53ZengGalenos Publishing HouseTurkish Journal of Hematology1308-52632018-02-01351424810.4274/tjh.2016.0498TJH-38039The Effect of Bone Marrow Mesenchymal Stem Cells on the Granulocytic Differentiation of HL-60 CellsHossein Nikkhah0Elham Safarzadeh1Karim Shamsasenjan2Mehdi Yousefi3Parisa Lotfinejad4Mehdi Talebi5Mozhde Mohammadian6Farhoud Golafshan7Ali Akbar Movassaghpour8Tabriz University Faculty of Medicine, Hematology and Oncology Research Center, Tabriz, IranTabriz University Faculty of Medicine, Drug Applied Research Center, Tabriz, Iran; Tabriz University Faculty of Medicine, Department of Immunology, Tabriz, IranTabriz University Faculty of Medicine, Hematology and Oncology Research Center, Tabriz, IranTabriz University Faculty of Medicine, Drug Applied Research Center, Tabriz, Iran; Tabriz University Faculty of Medicine, Department of Immunology, Tabriz, IranTabriz University Faculty of Medicine, Hematology and Oncology Research Center, Tabriz, Iran; Tabriz University Faculty of Medicine, Department of Immunology, Tabriz, IranTabriz University Faculty of Medicine, Hematology and Oncology Research Center, Tabriz, IranMazandaran University Faculty of Medicine, Amol Faculty of Paramedical Sciences, Sari, IranHamline University Faculty of Medicine, Department of Biology, Minnesota, USATabriz University Faculty of Medicine, Hematology and Oncology Research Center, Tabriz, IranObjective: Mesenchymal stem cells (MSCs) are multipotent stromal cells that can differentiate into a variety of cell types. They control the process of hematopoiesis by secreting regulatory cytokines and growth factors and by the expression of important cell adhesion molecules for cell-to-cell interactions. This investigation was intended to examine the effect of bone marrow (BM)-derived MSCs on the differentiation of HL-60 cells according to morphological evaluation, flow cytometry analysis, and gene expression profile. Materials and Methods: The BM-MSCs were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (FBS). After the third passage, the BM-MSCs were irradiated at 30 Gy. To compare how the HL-60 cells differentiated in groups treated differently, HL-60 cells were cultured in RPMI-1640 and supplemented with 10% FBS. The HL-60 cells were seeded into six-well culture plates and treated with all-trans-retinoic acid (ATRA), BM-MSCs, or BM-MSCs in combination with ATRA, while one well remained as untreated HL-60 cells. The expression levels of the granulocyte subsetspecific genes in the HL-60 cells were assayed by real-time polymerase chain reaction. Results: Our results revealed that BM-MSCs support the granulocytic differentiation of the human promyelocytic leukemia cell line HL-60. Conclusion: Based on the results of this study, we concluded that BM-MSCs may be an effective resource in reducing or even preventing ATRA's side effects and may promote differentiation for short medication periods. Though BM-MSCs are effective resources, more complementary studies are necessary to improve this differentiation mechanism in clinical cases.https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-38039mesenchymal stem cellshl-60 cellsdifferentiationalltrans-retinoic acid |
| spellingShingle | Hossein Nikkhah Elham Safarzadeh Karim Shamsasenjan Mehdi Yousefi Parisa Lotfinejad Mehdi Talebi Mozhde Mohammadian Farhoud Golafshan Ali Akbar Movassaghpour The Effect of Bone Marrow Mesenchymal Stem Cells on the Granulocytic Differentiation of HL-60 Cells Turkish Journal of Hematology mesenchymal stem cells hl-60 cells differentiation alltrans-retinoic acid |
| title | The Effect of Bone Marrow Mesenchymal Stem Cells on the Granulocytic Differentiation of HL-60 Cells |
| title_full | The Effect of Bone Marrow Mesenchymal Stem Cells on the Granulocytic Differentiation of HL-60 Cells |
| title_fullStr | The Effect of Bone Marrow Mesenchymal Stem Cells on the Granulocytic Differentiation of HL-60 Cells |
| title_full_unstemmed | The Effect of Bone Marrow Mesenchymal Stem Cells on the Granulocytic Differentiation of HL-60 Cells |
| title_short | The Effect of Bone Marrow Mesenchymal Stem Cells on the Granulocytic Differentiation of HL-60 Cells |
| title_sort | effect of bone marrow mesenchymal stem cells on the granulocytic differentiation of hl 60 cells |
| topic | mesenchymal stem cells hl-60 cells differentiation alltrans-retinoic acid |
| url | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-38039 |
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