Diverse eggs, diverse species? Production of two egg morphotypes in Paramacrobiotus bifrons, a new eutardigrade species within the areolatus group
AbstractSpecies within the eutardigrade superfamily Macrobiotoidea are usually differentiated through their eggshell morphology. However, this trait can show very high intraspecific variability and, as consequence, species delimitation is challenging without molecular support. Although eggshell vari...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-01-01
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| Series: | The European Zoological Journal |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/24750263.2024.2317465 |
| _version_ | 1797278764623724544 |
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| author | S. Brandoli M. Cesari E. Massa M. Vecchi L. Rebecchi R. Guidetti |
| author_facet | S. Brandoli M. Cesari E. Massa M. Vecchi L. Rebecchi R. Guidetti |
| author_sort | S. Brandoli |
| collection | DOAJ |
| description | AbstractSpecies within the eutardigrade superfamily Macrobiotoidea are usually differentiated through their eggshell morphology. However, this trait can show very high intraspecific variability and, as consequence, species delimitation is challenging without molecular support. Although eggshell variability usually consists of small details, cases of species with very different egg morphotypes are known. Paramacrobiotus bifrons sp. nov. here described with an integrative approach, represents one of the species with extreme variability in eggshell morphology and it belongs to the areolatus group. The production by this gonochoric species of both areolatus-type and csotiensis-type eggs, the latter being rare in the group but most abundant in this species, was assessed by DNA barcoding, culturing, and inter-morphotype crosses. The reason underlying the production of two different eggshells resulted not related to tested culturing conditions, seasonality, or male’s influence. Confocal (with and without staining), Light, and Scanning Electron Microscopies analyses of the two egg morphotypes laid by P. bifrons sp. nov. allowed to redescribe the csotiensis-type egg, shed light on its composition (excluding chitin as a component), and to find shared details supporting the belonging of the two egg morphotypes to the same species.http://www.zoobank.org/urn:lsid:zoobank.org:pub:E9519638-2CE9-414D-B96B-0F403845D4CAhttp://www.zoobank.org/urn:lsid:zoobank.org:act:82DDACA5-F539-4A80-8418-8AF4A370B631 |
| first_indexed | 2024-03-07T16:13:56Z |
| format | Article |
| id | doaj.art-6725c92f59d24b9c86c0ad10d55899f6 |
| institution | Directory Open Access Journal |
| issn | 2475-0263 |
| language | English |
| last_indexed | 2024-03-07T16:13:56Z |
| publishDate | 2024-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | The European Zoological Journal |
| spelling | doaj.art-6725c92f59d24b9c86c0ad10d55899f62024-03-04T15:55:20ZengTaylor & Francis GroupThe European Zoological Journal2475-02632024-01-0191127429710.1080/24750263.2024.2317465Diverse eggs, diverse species? Production of two egg morphotypes in Paramacrobiotus bifrons, a new eutardigrade species within the areolatus groupS. Brandoli0M. Cesari1E. Massa2M. Vecchi3L. Rebecchi4R. Guidetti5Department of Life Sciences, University of Modena and Reggio Emilia, Modena, ItalyDepartment of Life Sciences, University of Modena and Reggio Emilia, Modena, ItalyDepartment of Life Sciences, University of Modena and Reggio Emilia, Modena, ItalyInstitute of Systematics and Evolution of Animals, Polish Academy of Sciences, Warszawa, PolandDepartment of Life Sciences, University of Modena and Reggio Emilia, Modena, ItalyDepartment of Life Sciences, University of Modena and Reggio Emilia, Modena, ItalyAbstractSpecies within the eutardigrade superfamily Macrobiotoidea are usually differentiated through their eggshell morphology. However, this trait can show very high intraspecific variability and, as consequence, species delimitation is challenging without molecular support. Although eggshell variability usually consists of small details, cases of species with very different egg morphotypes are known. Paramacrobiotus bifrons sp. nov. here described with an integrative approach, represents one of the species with extreme variability in eggshell morphology and it belongs to the areolatus group. The production by this gonochoric species of both areolatus-type and csotiensis-type eggs, the latter being rare in the group but most abundant in this species, was assessed by DNA barcoding, culturing, and inter-morphotype crosses. The reason underlying the production of two different eggshells resulted not related to tested culturing conditions, seasonality, or male’s influence. Confocal (with and without staining), Light, and Scanning Electron Microscopies analyses of the two egg morphotypes laid by P. bifrons sp. nov. allowed to redescribe the csotiensis-type egg, shed light on its composition (excluding chitin as a component), and to find shared details supporting the belonging of the two egg morphotypes to the same species.http://www.zoobank.org/urn:lsid:zoobank.org:pub:E9519638-2CE9-414D-B96B-0F403845D4CAhttp://www.zoobank.org/urn:lsid:zoobank.org:act:82DDACA5-F539-4A80-8418-8AF4A370B631https://www.tandfonline.com/doi/10.1080/24750263.2024.2317465EggshellcsotiensisinterbreedingkaryologyTardigrada |
| spellingShingle | S. Brandoli M. Cesari E. Massa M. Vecchi L. Rebecchi R. Guidetti Diverse eggs, diverse species? Production of two egg morphotypes in Paramacrobiotus bifrons, a new eutardigrade species within the areolatus group The European Zoological Journal Eggshell csotiensis interbreeding karyology Tardigrada |
| title | Diverse eggs, diverse species? Production of two egg morphotypes in Paramacrobiotus bifrons, a new eutardigrade species within the areolatus group |
| title_full | Diverse eggs, diverse species? Production of two egg morphotypes in Paramacrobiotus bifrons, a new eutardigrade species within the areolatus group |
| title_fullStr | Diverse eggs, diverse species? Production of two egg morphotypes in Paramacrobiotus bifrons, a new eutardigrade species within the areolatus group |
| title_full_unstemmed | Diverse eggs, diverse species? Production of two egg morphotypes in Paramacrobiotus bifrons, a new eutardigrade species within the areolatus group |
| title_short | Diverse eggs, diverse species? Production of two egg morphotypes in Paramacrobiotus bifrons, a new eutardigrade species within the areolatus group |
| title_sort | diverse eggs diverse species production of two egg morphotypes in paramacrobiotus bifrons a new eutardigrade species within the areolatus group |
| topic | Eggshell csotiensis interbreeding karyology Tardigrada |
| url | https://www.tandfonline.com/doi/10.1080/24750263.2024.2317465 |
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