Genetic aspects of HPV infection detection in tumor and adjacent tissues of patients with laryngeal squamous cell carcinoma
The article describes methods for the human papillomavirus (HPV) detection in tumor and adjacent (morphologically intact) tissues of patients with laryngeal squamous cell carcinoma (LSSC) in terms of viral pathogenesis. Comparative evaluation of the principles and techniques for HPV detection was pe...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Central Research Institute for Epidemiology
2016-12-01
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| Series: | Вопросы вирусологии |
| Subjects: | |
| Online Access: | https://virusjour.crie.ru/jour/article/viewFile/107/38 |
| _version_ | 1797782208075792384 |
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| author | O. Yu. Dvoryaninova E. G. Nikitina V. A. Bychkov N. V. Litviakov |
| author_facet | O. Yu. Dvoryaninova E. G. Nikitina V. A. Bychkov N. V. Litviakov |
| author_sort | O. Yu. Dvoryaninova |
| collection | DOAJ |
| description | The article describes methods for the human papillomavirus (HPV) detection in tumor and adjacent (morphologically intact) tissues of patients with laryngeal squamous cell carcinoma (LSSC) in terms of viral pathogenesis. Comparative evaluation of the principles and techniques for HPV detection was performed. Advantages and disadvantages of the HPV detection methods are described. Approaches for DNA and HPV oncoproteins E6-E7 identification are substantiated. The results of our research into the qualitative and quantitative detection of HPV in the tumor and adjacent tissues of patients with Lssc are described. The research was conducted using commercial test systems Amplisens HPV HR screen-titre-FL and Amplisens HPV HR genotype-FL. Based on these results we developed the algorithm of HPV detection in samples of tumor tissue of patients with Lssc. The need for typing HPV-positive tissue samples with low concentration of HPV DNA was discussed. |
| first_indexed | 2024-03-13T00:07:45Z |
| format | Article |
| id | doaj.art-6726042651f54a4bbbcfa24a12ff84bd |
| institution | Directory Open Access Journal |
| issn | 0507-4088 2411-2097 |
| language | English |
| last_indexed | 2024-03-13T00:07:45Z |
| publishDate | 2016-12-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Вопросы вирусологии |
| spelling | doaj.art-6726042651f54a4bbbcfa24a12ff84bd2023-07-12T20:03:23ZengCentral Research Institute for EpidemiologyВопросы вирусологии0507-40882411-20972016-12-0161627527910.18821/0507-4088-2016-61-6-275-27988Genetic aspects of HPV infection detection in tumor and adjacent tissues of patients with laryngeal squamous cell carcinomaO. Yu. Dvoryaninova0E. G. Nikitina1V. A. Bychkov2N. V. Litviakov3Tomsk Cancer Research InstituteTomsk Cancer Research InstituteTomsk Cancer Research InstituteTomsk Cancer Research InstituteThe article describes methods for the human papillomavirus (HPV) detection in tumor and adjacent (morphologically intact) tissues of patients with laryngeal squamous cell carcinoma (LSSC) in terms of viral pathogenesis. Comparative evaluation of the principles and techniques for HPV detection was performed. Advantages and disadvantages of the HPV detection methods are described. Approaches for DNA and HPV oncoproteins E6-E7 identification are substantiated. The results of our research into the qualitative and quantitative detection of HPV in the tumor and adjacent tissues of patients with Lssc are described. The research was conducted using commercial test systems Amplisens HPV HR screen-titre-FL and Amplisens HPV HR genotype-FL. Based on these results we developed the algorithm of HPV detection in samples of tumor tissue of patients with Lssc. The need for typing HPV-positive tissue samples with low concentration of HPV DNA was discussed.https://virusjour.crie.ru/jour/article/viewFile/107/38laryngeal squamous cell carcinomatumor and adjacent tissuehuman papillomaviruscommercial test system amplisens hpv hr screen-titre-fl and amplisens hpv hr genotype-flmolecular-genetic methods for hpv detection |
| spellingShingle | O. Yu. Dvoryaninova E. G. Nikitina V. A. Bychkov N. V. Litviakov Genetic aspects of HPV infection detection in tumor and adjacent tissues of patients with laryngeal squamous cell carcinoma Вопросы вирусологии laryngeal squamous cell carcinoma tumor and adjacent tissue human papillomavirus commercial test system amplisens hpv hr screen-titre-fl and amplisens hpv hr genotype-fl molecular-genetic methods for hpv detection |
| title | Genetic aspects of HPV infection detection in tumor and adjacent tissues of patients with laryngeal squamous cell carcinoma |
| title_full | Genetic aspects of HPV infection detection in tumor and adjacent tissues of patients with laryngeal squamous cell carcinoma |
| title_fullStr | Genetic aspects of HPV infection detection in tumor and adjacent tissues of patients with laryngeal squamous cell carcinoma |
| title_full_unstemmed | Genetic aspects of HPV infection detection in tumor and adjacent tissues of patients with laryngeal squamous cell carcinoma |
| title_short | Genetic aspects of HPV infection detection in tumor and adjacent tissues of patients with laryngeal squamous cell carcinoma |
| title_sort | genetic aspects of hpv infection detection in tumor and adjacent tissues of patients with laryngeal squamous cell carcinoma |
| topic | laryngeal squamous cell carcinoma tumor and adjacent tissue human papillomavirus commercial test system amplisens hpv hr screen-titre-fl and amplisens hpv hr genotype-fl molecular-genetic methods for hpv detection |
| url | https://virusjour.crie.ru/jour/article/viewFile/107/38 |
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