Purification and transcriptomic characterization of proliferative cells of Mesocestoides corti selectively affected by irradiation
Flatworms depend on stem cells for continued tissue growth and renewal during their life cycles, making these cells valuable drug targets. While neoblasts are extensively characterized in the free-living planarian Schmidtea mediterranea, and similar stem cells have been characterized in the trematod...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Parasitology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpara.2024.1362199/full |
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| author | Alicia Costábile María Fernanda Domínguez Inés Guarnaschelli Matías Preza Uriel Koziol Estela Castillo José F. Tort |
| author_facet | Alicia Costábile María Fernanda Domínguez Inés Guarnaschelli Matías Preza Uriel Koziol Estela Castillo José F. Tort |
| author_sort | Alicia Costábile |
| collection | DOAJ |
| description | Flatworms depend on stem cells for continued tissue growth and renewal during their life cycles, making these cells valuable drug targets. While neoblasts are extensively characterized in the free-living planarian Schmidtea mediterranea, and similar stem cells have been characterized in the trematode Schistosoma mansoni, their identification and characterization in cestodes is just emerging. Since stem cells are generally affected by irradiation, in this work we used this experimental approach to study the stem cells of the model cestode Mesocestoides corti. We found that gamma irradiation produces a dose-dependent decrease in proliferative cells, requiring higher doses than in other flatworms to completely abolish proliferation. The treatment results in the downregulation of candidate marker genes. Transcriptomic studies reveal that several genes downregulated after irradiation are conserved with other flatworms, and are related to cell cycle, DNA replication and repair functions. Furthermore, proliferative cells were isolated by cell sorting and also characterized transcriptomically. We found that the set of genes characteristic of proliferative cells agrees well with those downregulated during irradiation, and have a significant overlap with those expressed in planarian neoblasts or S. mansoni stem cells. Our study highlights that conserved mechanisms of stem cell biology may be functional in flatworms, suggesting that these could be relevant targets to evaluate in the control of parasitic species. |
| first_indexed | 2024-03-07T15:44:30Z |
| format | Article |
| id | doaj.art-6728c0c505314105beb814717997665e |
| institution | Directory Open Access Journal |
| issn | 2813-2424 |
| language | English |
| last_indexed | 2024-03-07T15:44:30Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Parasitology |
| spelling | doaj.art-6728c0c505314105beb814717997665e2024-03-05T04:51:55ZengFrontiers Media S.A.Frontiers in Parasitology2813-24242024-03-01310.3389/fpara.2024.13621991362199Purification and transcriptomic characterization of proliferative cells of Mesocestoides corti selectively affected by irradiationAlicia Costábile0María Fernanda Domínguez1Inés Guarnaschelli2Matías Preza3Uriel Koziol4Estela Castillo5José F. Tort6Sección Bioquímica, Facultad de Ciencias, Universidad de la República, Montevideo, UruguayDepartamento de Genética, Facultad de Medicina, Universidad de la República, Montevideo, UruguaySección Biología Celular, Facultad de Ciencias, Universidad de la República, Montevideo, UruguaySección Biología Celular, Facultad de Ciencias, Universidad de la República, Montevideo, UruguaySección Biología Celular, Facultad de Ciencias, Universidad de la República, Montevideo, UruguayUnidad de Biología Parasitaria, Facultad de Ciencias- Instituto de Higiene, Universidad de la República, Montevideo, UruguayDepartamento de Genética, Facultad de Medicina, Universidad de la República, Montevideo, UruguayFlatworms depend on stem cells for continued tissue growth and renewal during their life cycles, making these cells valuable drug targets. While neoblasts are extensively characterized in the free-living planarian Schmidtea mediterranea, and similar stem cells have been characterized in the trematode Schistosoma mansoni, their identification and characterization in cestodes is just emerging. Since stem cells are generally affected by irradiation, in this work we used this experimental approach to study the stem cells of the model cestode Mesocestoides corti. We found that gamma irradiation produces a dose-dependent decrease in proliferative cells, requiring higher doses than in other flatworms to completely abolish proliferation. The treatment results in the downregulation of candidate marker genes. Transcriptomic studies reveal that several genes downregulated after irradiation are conserved with other flatworms, and are related to cell cycle, DNA replication and repair functions. Furthermore, proliferative cells were isolated by cell sorting and also characterized transcriptomically. We found that the set of genes characteristic of proliferative cells agrees well with those downregulated during irradiation, and have a significant overlap with those expressed in planarian neoblasts or S. mansoni stem cells. Our study highlights that conserved mechanisms of stem cell biology may be functional in flatworms, suggesting that these could be relevant targets to evaluate in the control of parasitic species.https://www.frontiersin.org/articles/10.3389/fpara.2024.1362199/fullcestodesgerminative cellsproliferationirradiationcell purificationstem cell markers |
| spellingShingle | Alicia Costábile María Fernanda Domínguez Inés Guarnaschelli Matías Preza Uriel Koziol Estela Castillo José F. Tort Purification and transcriptomic characterization of proliferative cells of Mesocestoides corti selectively affected by irradiation Frontiers in Parasitology cestodes germinative cells proliferation irradiation cell purification stem cell markers |
| title | Purification and transcriptomic characterization of proliferative cells of Mesocestoides corti selectively affected by irradiation |
| title_full | Purification and transcriptomic characterization of proliferative cells of Mesocestoides corti selectively affected by irradiation |
| title_fullStr | Purification and transcriptomic characterization of proliferative cells of Mesocestoides corti selectively affected by irradiation |
| title_full_unstemmed | Purification and transcriptomic characterization of proliferative cells of Mesocestoides corti selectively affected by irradiation |
| title_short | Purification and transcriptomic characterization of proliferative cells of Mesocestoides corti selectively affected by irradiation |
| title_sort | purification and transcriptomic characterization of proliferative cells of mesocestoides corti selectively affected by irradiation |
| topic | cestodes germinative cells proliferation irradiation cell purification stem cell markers |
| url | https://www.frontiersin.org/articles/10.3389/fpara.2024.1362199/full |
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