Spore Production by Biocontrol Agent Trichoderma Harzianum in Submerged Fermentation: Effect of Agitation and Aeration

Effects of agitation and aeration on spore production and spore viability by biocontrol agent UPM 29 were investigated. The optimum fermentation at different agitation speeds, ranging from 300 rpm to 700 rpm show that there is an optimum agitation speed gives the highest spore production. The spore...

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Main Author: Syahiddin Dahlan Said
Format: Article
Language:English
Published: Universitas Syiah Kuala, Chemical Engineering Department 2007-12-01
Series:Jurnal Rekayasa Kimia & Lingkungan
Online Access:https://jurnal.usk.ac.id/RKL/article/view/1076
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author Syahiddin Dahlan Said
author_facet Syahiddin Dahlan Said
author_sort Syahiddin Dahlan Said
collection DOAJ
description Effects of agitation and aeration on spore production and spore viability by biocontrol agent UPM 29 were investigated. The optimum fermentation at different agitation speeds, ranging from 300 rpm to 700 rpm show that there is an optimum agitation speed gives the highest spore production. The spore production increased with increasing agitation speed from 300 rpm to 500 rpm. At the higher agitation speed the spore production dropped. The highest spore concentration (9.2x107 spores/ml) was produced in culture grown on media with agitation speed of 500 rpm. The rate of aeration effected spore production and spore productivity in the range 1.5 to 2.0 vvm, and increased the spore production from 4.7x107 to 6.0x107 spores/ml (28% increase) and 4.7x108 to 8.1 x108 spores /l/h respectively. The spore viability was not affected significantly by aeration rate or agitation speed. Keywords: agitation, biocontrol agent, biofungicide, spore viability, Trichoderma harzianum
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spelling doaj.art-672ceedb56174ef282c346a85de1384e2024-04-03T09:48:28ZengUniversitas Syiah Kuala, Chemical Engineering DepartmentJurnal Rekayasa Kimia & Lingkungan1412-50642356-16612007-12-016271761038Spore Production by Biocontrol Agent Trichoderma Harzianum in Submerged Fermentation: Effect of Agitation and AerationSyahiddin Dahlan Said0Department of Chemical Engineering, Faculty of Engineering, Syiah Kuala, UniversityEffects of agitation and aeration on spore production and spore viability by biocontrol agent UPM 29 were investigated. The optimum fermentation at different agitation speeds, ranging from 300 rpm to 700 rpm show that there is an optimum agitation speed gives the highest spore production. The spore production increased with increasing agitation speed from 300 rpm to 500 rpm. At the higher agitation speed the spore production dropped. The highest spore concentration (9.2x107 spores/ml) was produced in culture grown on media with agitation speed of 500 rpm. The rate of aeration effected spore production and spore productivity in the range 1.5 to 2.0 vvm, and increased the spore production from 4.7x107 to 6.0x107 spores/ml (28% increase) and 4.7x108 to 8.1 x108 spores /l/h respectively. The spore viability was not affected significantly by aeration rate or agitation speed. Keywords: agitation, biocontrol agent, biofungicide, spore viability, Trichoderma harzianumhttps://jurnal.usk.ac.id/RKL/article/view/1076
spellingShingle Syahiddin Dahlan Said
Spore Production by Biocontrol Agent Trichoderma Harzianum in Submerged Fermentation: Effect of Agitation and Aeration
Jurnal Rekayasa Kimia & Lingkungan
title Spore Production by Biocontrol Agent Trichoderma Harzianum in Submerged Fermentation: Effect of Agitation and Aeration
title_full Spore Production by Biocontrol Agent Trichoderma Harzianum in Submerged Fermentation: Effect of Agitation and Aeration
title_fullStr Spore Production by Biocontrol Agent Trichoderma Harzianum in Submerged Fermentation: Effect of Agitation and Aeration
title_full_unstemmed Spore Production by Biocontrol Agent Trichoderma Harzianum in Submerged Fermentation: Effect of Agitation and Aeration
title_short Spore Production by Biocontrol Agent Trichoderma Harzianum in Submerged Fermentation: Effect of Agitation and Aeration
title_sort spore production by biocontrol agent trichoderma harzianum in submerged fermentation effect of agitation and aeration
url https://jurnal.usk.ac.id/RKL/article/view/1076
work_keys_str_mv AT syahiddindahlansaid sporeproductionbybiocontrolagenttrichodermaharzianuminsubmergedfermentationeffectofagitationandaeration