Pectenovarin, A New Ovarian Carotenoprotein from Japanese Scallop <i>Mizuhopecten yessoensis</i>
The scallop <i>Mizuhopecten yessoensis</i> accumulates carotenoids in the ovary during the maturation stage. Its conspicuous pink color implies the presence of carotenoprotein. However, the carotenoprotein from the scallop ovary has never been isolated and characterized, probably due to...
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| Format: | Article |
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MDPI AG
2020-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/13/3042 |
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| author | Satoko Matsunaga Hiroki Ikeda Ryuichi Sakai |
| author_facet | Satoko Matsunaga Hiroki Ikeda Ryuichi Sakai |
| author_sort | Satoko Matsunaga |
| collection | DOAJ |
| description | The scallop <i>Mizuhopecten yessoensis</i> accumulates carotenoids in the ovary during the maturation stage. Its conspicuous pink color implies the presence of carotenoprotein. However, the carotenoprotein from the scallop ovary has never been isolated and characterized, probably due to its instability and complexity. Here, we developed an extraction and isolation procedure for the carotenoprotein by employing a basic buffer containing potassium bromide to facilitate its efficient extraction from the ovary, and we succeeded in obtaining the carotenoprotein, termed pectenovarin. The carotenoid composition of the pectenovarin was similar to that of the ovary. The N-terminal and internal amino acid sequences of pectenovarin showed a high similarity to those of vitellogenin, the precursor of egg yolk protein under analysis. |
| first_indexed | 2024-03-10T18:42:30Z |
| format | Article |
| id | doaj.art-672d28743906465da55b99156675f812 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T18:42:30Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj.art-672d28743906465da55b99156675f8122023-11-20T05:45:52ZengMDPI AGMolecules1420-30492020-07-012513304210.3390/molecules25133042Pectenovarin, A New Ovarian Carotenoprotein from Japanese Scallop <i>Mizuhopecten yessoensis</i>Satoko Matsunaga0Hiroki Ikeda1Ryuichi Sakai2Department of Material and Environmental Engineering, National Institute of Technology HAKODATE College, 14-1 Tokura-cho, Hakodate, Hokkaido 042-8501, JapanFaculty and Graduate School of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho, Hakodate 041-8611, JapanFaculty and Graduate School of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho, Hakodate 041-8611, JapanThe scallop <i>Mizuhopecten yessoensis</i> accumulates carotenoids in the ovary during the maturation stage. Its conspicuous pink color implies the presence of carotenoprotein. However, the carotenoprotein from the scallop ovary has never been isolated and characterized, probably due to its instability and complexity. Here, we developed an extraction and isolation procedure for the carotenoprotein by employing a basic buffer containing potassium bromide to facilitate its efficient extraction from the ovary, and we succeeded in obtaining the carotenoprotein, termed pectenovarin. The carotenoid composition of the pectenovarin was similar to that of the ovary. The N-terminal and internal amino acid sequences of pectenovarin showed a high similarity to those of vitellogenin, the precursor of egg yolk protein under analysis.https://www.mdpi.com/1420-3049/25/13/3042carotenoproteinscarotenoidsscallop ovaryJapanese scallop |
| spellingShingle | Satoko Matsunaga Hiroki Ikeda Ryuichi Sakai Pectenovarin, A New Ovarian Carotenoprotein from Japanese Scallop <i>Mizuhopecten yessoensis</i> Molecules carotenoproteins carotenoids scallop ovary Japanese scallop |
| title | Pectenovarin, A New Ovarian Carotenoprotein from Japanese Scallop <i>Mizuhopecten yessoensis</i> |
| title_full | Pectenovarin, A New Ovarian Carotenoprotein from Japanese Scallop <i>Mizuhopecten yessoensis</i> |
| title_fullStr | Pectenovarin, A New Ovarian Carotenoprotein from Japanese Scallop <i>Mizuhopecten yessoensis</i> |
| title_full_unstemmed | Pectenovarin, A New Ovarian Carotenoprotein from Japanese Scallop <i>Mizuhopecten yessoensis</i> |
| title_short | Pectenovarin, A New Ovarian Carotenoprotein from Japanese Scallop <i>Mizuhopecten yessoensis</i> |
| title_sort | pectenovarin a new ovarian carotenoprotein from japanese scallop i mizuhopecten yessoensis i |
| topic | carotenoproteins carotenoids scallop ovary Japanese scallop |
| url | https://www.mdpi.com/1420-3049/25/13/3042 |
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