Co-administration of amygdalin and deoxynivalenol disrupted regulatory proteins linked to proliferation of porcine ovarian cells in vitro
<p>Deoxynivalenol (DON) represents one of the most prevalent trichothecene mycotoxin produced by <em>Fusarium </em>species, causing economic and health impacts. On the other hand, amygdalin has been demonstrated to possess both prophylactic and curative properties, thus it has been...
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| Format: | Article |
| Language: | English |
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HACCP Consulting
2017-01-01
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| Series: | Potravinarstvo |
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| Online Access: | http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/791 |
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| author | Marek Halenár Marína Medveďová Simona Baldovská Katarína Michalcová Adriana Kolesárová |
| author_facet | Marek Halenár Marína Medveďová Simona Baldovská Katarína Michalcová Adriana Kolesárová |
| author_sort | Marek Halenár |
| collection | DOAJ |
| description | <p>Deoxynivalenol (DON) represents one of the most prevalent trichothecene mycotoxin produced by <em>Fusarium </em>species, causing economic and health impacts. On the other hand, amygdalin has been demonstrated to possess both prophylactic and curative properties, thus it has been used as a traditional drug because of its wide range of medicinal benefits, including curing or preventing cancer, relieving fever, suppressing cough, and quenching thirst. The aim of this <em>in vitro</em> study was to evaluate potential effects of natural product amygdalin combined with mycotoxin deoxynivalenol (DON) on the key regulators of cell proliferation and apoptosis in porcine ovarian granulosa cells. Ovarian granulosa cells were incubated for 24h with amygdalin (1, 10, 100, 1000, 10 000 μg.mL<sup>-1</sup>) combined with deoxynivalenol (1 μg.mL<sup>-1</sup>), while the control group remained untreated. The presence of proliferative (cyclin B1, PCNA) and apoptotic markers (caspase-3) in porcine ovarian granulosa cells after amygdalin treatment (1, 10, 100, 1000, 10 000 μg.mL<sup>-1</sup>) combined with deoxynivalneol (1 μg.mL<sup>-1</sup>) was detected by immunocytochemistry. The presence of proliferative (cyclin B1, PCNA) and apoptotic markers (caspase-3) in porcine ovarian granulosa cells was detected by immunocytochemistry. Co-administration of amygdalin plus DON significantly (<em>p</em> <em><</em>0.05) increased the number of granulosa cells containing cyclin B1 and PCNA at all tested concetrations, when compared to control. However, percentage of granulosa cells containing major apoptotic marker caspase-3 did not differ after co-administration of amygdalin and DON. In summary, results form this in vitro study indicate that co-exposure of amygdalin and deoxynivalenol may act to stimulate proliferation-associated peptides in porcine ovarian granulosa cells, and thus alter cell proliferation and normal follicular development.</p> |
| first_indexed | 2024-12-10T16:00:40Z |
| format | Article |
| id | doaj.art-672f3db55da04d058f044cf355e2ccc6 |
| institution | Directory Open Access Journal |
| issn | 1337-0960 |
| language | English |
| last_indexed | 2024-12-10T16:00:40Z |
| publishDate | 2017-01-01 |
| publisher | HACCP Consulting |
| record_format | Article |
| series | Potravinarstvo |
| spelling | doaj.art-672f3db55da04d058f044cf355e2ccc62022-12-22T01:42:26ZengHACCP ConsultingPotravinarstvo1337-09602017-01-0111150350910.5219/791583Co-administration of amygdalin and deoxynivalenol disrupted regulatory proteins linked to proliferation of porcine ovarian cells in vitroMarek Halenár0Marína Medveďová1Simona Baldovská2Katarína Michalcová3Adriana Kolesárová4Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Animal Physiology, Tr. A. Hlinku 2, 949 76 NitraSlovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Animal Physiology, Tr. A. Hlinku 2, 949 76 NitraSlovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Animal Physiology, Tr. A. Hlinku 2, 949 76 NitraSlovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Animal Physiology, Tr. A. Hlinku 2, 949 76 NitraSlovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Animal Physiology, Tr. A. Hlinku 2, 949 76 Nitra<p>Deoxynivalenol (DON) represents one of the most prevalent trichothecene mycotoxin produced by <em>Fusarium </em>species, causing economic and health impacts. On the other hand, amygdalin has been demonstrated to possess both prophylactic and curative properties, thus it has been used as a traditional drug because of its wide range of medicinal benefits, including curing or preventing cancer, relieving fever, suppressing cough, and quenching thirst. The aim of this <em>in vitro</em> study was to evaluate potential effects of natural product amygdalin combined with mycotoxin deoxynivalenol (DON) on the key regulators of cell proliferation and apoptosis in porcine ovarian granulosa cells. Ovarian granulosa cells were incubated for 24h with amygdalin (1, 10, 100, 1000, 10 000 μg.mL<sup>-1</sup>) combined with deoxynivalenol (1 μg.mL<sup>-1</sup>), while the control group remained untreated. The presence of proliferative (cyclin B1, PCNA) and apoptotic markers (caspase-3) in porcine ovarian granulosa cells after amygdalin treatment (1, 10, 100, 1000, 10 000 μg.mL<sup>-1</sup>) combined with deoxynivalneol (1 μg.mL<sup>-1</sup>) was detected by immunocytochemistry. The presence of proliferative (cyclin B1, PCNA) and apoptotic markers (caspase-3) in porcine ovarian granulosa cells was detected by immunocytochemistry. Co-administration of amygdalin plus DON significantly (<em>p</em> <em><</em>0.05) increased the number of granulosa cells containing cyclin B1 and PCNA at all tested concetrations, when compared to control. However, percentage of granulosa cells containing major apoptotic marker caspase-3 did not differ after co-administration of amygdalin and DON. In summary, results form this in vitro study indicate that co-exposure of amygdalin and deoxynivalenol may act to stimulate proliferation-associated peptides in porcine ovarian granulosa cells, and thus alter cell proliferation and normal follicular development.</p>http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/791amygdalindeoxynivalenolovarian cellproliferationapoptosis |
| spellingShingle | Marek Halenár Marína Medveďová Simona Baldovská Katarína Michalcová Adriana Kolesárová Co-administration of amygdalin and deoxynivalenol disrupted regulatory proteins linked to proliferation of porcine ovarian cells in vitro Potravinarstvo amygdalin deoxynivalenol ovarian cell proliferation apoptosis |
| title | Co-administration of amygdalin and deoxynivalenol disrupted regulatory proteins linked to proliferation of porcine ovarian cells in vitro |
| title_full | Co-administration of amygdalin and deoxynivalenol disrupted regulatory proteins linked to proliferation of porcine ovarian cells in vitro |
| title_fullStr | Co-administration of amygdalin and deoxynivalenol disrupted regulatory proteins linked to proliferation of porcine ovarian cells in vitro |
| title_full_unstemmed | Co-administration of amygdalin and deoxynivalenol disrupted regulatory proteins linked to proliferation of porcine ovarian cells in vitro |
| title_short | Co-administration of amygdalin and deoxynivalenol disrupted regulatory proteins linked to proliferation of porcine ovarian cells in vitro |
| title_sort | co administration of amygdalin and deoxynivalenol disrupted regulatory proteins linked to proliferation of porcine ovarian cells in vitro |
| topic | amygdalin deoxynivalenol ovarian cell proliferation apoptosis |
| url | http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/791 |
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