Induction of silencing in plants by high-pressure spraying of in vitro-synthesized small RNAs

Induction of silencing in plants by high-pressure spraying of in vitro-synthesized small RNAs

In this report we describe a method for the delivery of small interfering RNAs (siRNAs) into plant cells. In vitro synthesized siRNAs that were designed to target the coding region of a GREEN FLUORESCENT PROTEIN (GFP) transgene were applied by various methods onto GFP-expressing transgenic Nicotiana...

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Bibliographic Details
Main Authors: Athanasios Dalakouras, Michèle Wassenegger, John McMillan, Vinitha Cardoza, Ira Maegele, Elena Dadami, Miriam Runne, Gabi Krczal, Michael Wassenegger
Format: Article
Language:English
Published: Frontiers Media S.A. 2016-08-01
Series:Frontiers in Plant Science
Subjects:
RNA silencing
Deep sequencing
GFP
Nicotiana benthamiana
siRNAs
spraying
Online Access:http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01327/full

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