Mass Spectrometry-Based Proteomics: Analyses Related to Drug-Resistance and Disease Biomarkers
Mass spectrometry-based proteomics is a key player in research efforts to characterize aberrant epigenetic alterations, including histone post-translational modifications and DNA methylation. Data generated by this approach complements and enrich datasets generated by genomic, epigenetic and transcr...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Medicina |
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| Online Access: | https://www.mdpi.com/1648-9144/59/10/1722 |
| _version_ | 1827720525451886592 |
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| author | Marco Agostini Pietro Traldi Mahmoud Hamdan |
| author_facet | Marco Agostini Pietro Traldi Mahmoud Hamdan |
| author_sort | Marco Agostini |
| collection | DOAJ |
| description | Mass spectrometry-based proteomics is a key player in research efforts to characterize aberrant epigenetic alterations, including histone post-translational modifications and DNA methylation. Data generated by this approach complements and enrich datasets generated by genomic, epigenetic and transcriptomics approaches. These combined datasets can provide much-needed information on various mechanisms responsible for drug resistance, the discovery and validation of potential biomarkers for different diseases, the identification of signaling pathways, and genes and enzymes to be targeted by future therapies. The increasing use of high-resolution, high-accuracy mass spectrometers combined with more refined protein labeling and enrichment procedures enhanced the role of this approach in the investigation of these epigenetic modifications. In this review, we discuss recent MS-based studies, which are contributing to current research efforts to understand certain mechanisms behind drug resistance to therapy. We also discuss how these MS-based analyses are contributing to biomarkers discovery and validation. |
| first_indexed | 2024-03-10T21:04:43Z |
| format | Article |
| id | doaj.art-67314541368a4596977ca532e13ca15e |
| institution | Directory Open Access Journal |
| issn | 1010-660X 1648-9144 |
| language | English |
| last_indexed | 2024-03-10T21:04:43Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Medicina |
| spelling | doaj.art-67314541368a4596977ca532e13ca15e2023-11-19T17:16:12ZengMDPI AGMedicina1010-660X1648-91442023-09-015910172210.3390/medicina59101722Mass Spectrometry-Based Proteomics: Analyses Related to Drug-Resistance and Disease BiomarkersMarco Agostini0Pietro Traldi1Mahmoud Hamdan2Istituto di Ricerca Pediatrica Città della Speranza, Corso Stati Uniti 4, 35100 Padova, ItalyIstituto di Ricerca Pediatrica Città della Speranza, Corso Stati Uniti 4, 35100 Padova, ItalyIstituto di Ricerca Pediatrica Città della Speranza, Corso Stati Uniti 4, 35100 Padova, ItalyMass spectrometry-based proteomics is a key player in research efforts to characterize aberrant epigenetic alterations, including histone post-translational modifications and DNA methylation. Data generated by this approach complements and enrich datasets generated by genomic, epigenetic and transcriptomics approaches. These combined datasets can provide much-needed information on various mechanisms responsible for drug resistance, the discovery and validation of potential biomarkers for different diseases, the identification of signaling pathways, and genes and enzymes to be targeted by future therapies. The increasing use of high-resolution, high-accuracy mass spectrometers combined with more refined protein labeling and enrichment procedures enhanced the role of this approach in the investigation of these epigenetic modifications. In this review, we discuss recent MS-based studies, which are contributing to current research efforts to understand certain mechanisms behind drug resistance to therapy. We also discuss how these MS-based analyses are contributing to biomarkers discovery and validation.https://www.mdpi.com/1648-9144/59/10/1722mass spectrometrypost-translational modificationsdrug resistance |
| spellingShingle | Marco Agostini Pietro Traldi Mahmoud Hamdan Mass Spectrometry-Based Proteomics: Analyses Related to Drug-Resistance and Disease Biomarkers Medicina mass spectrometry post-translational modifications drug resistance |
| title | Mass Spectrometry-Based Proteomics: Analyses Related to Drug-Resistance and Disease Biomarkers |
| title_full | Mass Spectrometry-Based Proteomics: Analyses Related to Drug-Resistance and Disease Biomarkers |
| title_fullStr | Mass Spectrometry-Based Proteomics: Analyses Related to Drug-Resistance and Disease Biomarkers |
| title_full_unstemmed | Mass Spectrometry-Based Proteomics: Analyses Related to Drug-Resistance and Disease Biomarkers |
| title_short | Mass Spectrometry-Based Proteomics: Analyses Related to Drug-Resistance and Disease Biomarkers |
| title_sort | mass spectrometry based proteomics analyses related to drug resistance and disease biomarkers |
| topic | mass spectrometry post-translational modifications drug resistance |
| url | https://www.mdpi.com/1648-9144/59/10/1722 |
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