Genetic Plurality of OXA/NDM-Encoding Features Characterized From Enterobacterales Recovered From Czech Hospitals
The aim of this study was to characterize four Enterobacterales co-producing NDM- and OXA-48-like carbapenemases from Czech patients with travel history or/and previous hospitalization abroad. Klebsiella pneumoniae isolates belonged to “high risk” clones ST147, ST11, and ST15, while the Escherichia...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-02-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.641415/full |
| _version_ | 1819059379978108928 |
|---|---|
| author | Katerina Chudejova Katerina Chudejova Lucie Kraftova Lucie Kraftova Vittoria Mattioni Marchetti Vittoria Mattioni Marchetti Jaroslav Hrabak Jaroslav Hrabak Costas C. Papagiannitsis Costas C. Papagiannitsis Costas C. Papagiannitsis Ibrahim Bitar Ibrahim Bitar |
| author_facet | Katerina Chudejova Katerina Chudejova Lucie Kraftova Lucie Kraftova Vittoria Mattioni Marchetti Vittoria Mattioni Marchetti Jaroslav Hrabak Jaroslav Hrabak Costas C. Papagiannitsis Costas C. Papagiannitsis Costas C. Papagiannitsis Ibrahim Bitar Ibrahim Bitar |
| author_sort | Katerina Chudejova |
| collection | DOAJ |
| description | The aim of this study was to characterize four Enterobacterales co-producing NDM- and OXA-48-like carbapenemases from Czech patients with travel history or/and previous hospitalization abroad. Klebsiella pneumoniae isolates belonged to “high risk” clones ST147, ST11, and ST15, while the Escherichia coli isolate was assigned to ST167. All isolates expressed resistance against most β-lactams, including carbapenems, while retaining susceptibility to colistin. Furthermore, analysis of WGS data showed that all four isolates co-produced OXA-48- and NDM-type carbapenemases, in different combinations (Kpn47733: blaNDM–5 + blaOXA–181; Kpn50595: blaNDM–1 + blaOXA–181; Kpn51015: blaNDM–1 + blaOXA–244; Eco52418: blaNDM–5 + blaOXA–244). In Kpn51015, the blaOXA–244 was found on plasmid p51015_OXA-244, while the respective gene was localized in the chromosomal contig of E. coli Eco52418. On the other hand, blaOXA–181 was identified on a ColKP3 plasmid in isolate Kpn47733, while a blaOXA–181-carrying plasmid being an IncX3-ColKP3 fusion was identified in Kpn50595. The blaNDM–1 gene was found on two different plasmids, p51015_NDM-1 belonging to a novel IncH plasmid group and p51015_NDM-1 being an IncFK1-FIB replicon. Furthermore, the blaNDM–5 was found in two IncFII plasmids exhibiting limited nucleotide similarity to each other. In both plasmids, the genetic environment of blaNDM–5 was identical. Finally, in all four carbapenemase-producing isolates, a diverse number of additional replicons, some of these associated with important resistance determinants, like blaCTX–M–15, arr-2 and ermB, were identified. In conclusion, this study reports the first description of OXA-244-producing Enterobacterales isolated from Czech hospitals. Additionally, our findings indicated the genetic plurality involved in the acquisition and dissemination of determinants encoding OXA/NDM carbapenemases. |
| first_indexed | 2024-12-21T14:10:10Z |
| format | Article |
| id | doaj.art-673372789eca4bb1b8ae3cfcd12cf1d0 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-21T14:10:10Z |
| publishDate | 2021-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-673372789eca4bb1b8ae3cfcd12cf1d02022-12-21T19:01:04ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-02-011210.3389/fmicb.2021.641415641415Genetic Plurality of OXA/NDM-Encoding Features Characterized From Enterobacterales Recovered From Czech HospitalsKaterina Chudejova0Katerina Chudejova1Lucie Kraftova2Lucie Kraftova3Vittoria Mattioni Marchetti4Vittoria Mattioni Marchetti5Jaroslav Hrabak6Jaroslav Hrabak7Costas C. Papagiannitsis8Costas C. Papagiannitsis9Costas C. Papagiannitsis10Ibrahim Bitar11Ibrahim Bitar12Department of Microbiology, Faculty of Medicine, University Hospital in Pilsen, Charles University, Pilsen, CzechiaBiomedical Center, Faculty of Medicine, Charles University, Pilsen, CzechiaDepartment of Microbiology, Faculty of Medicine, University Hospital in Pilsen, Charles University, Pilsen, CzechiaBiomedical Center, Faculty of Medicine, Charles University, Pilsen, CzechiaDepartment of Microbiology, Faculty of Medicine, University Hospital in Pilsen, Charles University, Pilsen, CzechiaBiomedical Center, Faculty of Medicine, Charles University, Pilsen, CzechiaDepartment of Microbiology, Faculty of Medicine, University Hospital in Pilsen, Charles University, Pilsen, CzechiaBiomedical Center, Faculty of Medicine, Charles University, Pilsen, CzechiaDepartment of Microbiology, Faculty of Medicine, University Hospital in Pilsen, Charles University, Pilsen, CzechiaBiomedical Center, Faculty of Medicine, Charles University, Pilsen, CzechiaDepartment of Microbiology, University Hospital of Larissa, Larissa, GreeceDepartment of Microbiology, Faculty of Medicine, University Hospital in Pilsen, Charles University, Pilsen, CzechiaBiomedical Center, Faculty of Medicine, Charles University, Pilsen, CzechiaThe aim of this study was to characterize four Enterobacterales co-producing NDM- and OXA-48-like carbapenemases from Czech patients with travel history or/and previous hospitalization abroad. Klebsiella pneumoniae isolates belonged to “high risk” clones ST147, ST11, and ST15, while the Escherichia coli isolate was assigned to ST167. All isolates expressed resistance against most β-lactams, including carbapenems, while retaining susceptibility to colistin. Furthermore, analysis of WGS data showed that all four isolates co-produced OXA-48- and NDM-type carbapenemases, in different combinations (Kpn47733: blaNDM–5 + blaOXA–181; Kpn50595: blaNDM–1 + blaOXA–181; Kpn51015: blaNDM–1 + blaOXA–244; Eco52418: blaNDM–5 + blaOXA–244). In Kpn51015, the blaOXA–244 was found on plasmid p51015_OXA-244, while the respective gene was localized in the chromosomal contig of E. coli Eco52418. On the other hand, blaOXA–181 was identified on a ColKP3 plasmid in isolate Kpn47733, while a blaOXA–181-carrying plasmid being an IncX3-ColKP3 fusion was identified in Kpn50595. The blaNDM–1 gene was found on two different plasmids, p51015_NDM-1 belonging to a novel IncH plasmid group and p51015_NDM-1 being an IncFK1-FIB replicon. Furthermore, the blaNDM–5 was found in two IncFII plasmids exhibiting limited nucleotide similarity to each other. In both plasmids, the genetic environment of blaNDM–5 was identical. Finally, in all four carbapenemase-producing isolates, a diverse number of additional replicons, some of these associated with important resistance determinants, like blaCTX–M–15, arr-2 and ermB, were identified. In conclusion, this study reports the first description of OXA-244-producing Enterobacterales isolated from Czech hospitals. Additionally, our findings indicated the genetic plurality involved in the acquisition and dissemination of determinants encoding OXA/NDM carbapenemases.https://www.frontiersin.org/articles/10.3389/fmicb.2021.641415/fullOXA-244OXA-181NDM-1NDM-5mobile genetic elements |
| spellingShingle | Katerina Chudejova Katerina Chudejova Lucie Kraftova Lucie Kraftova Vittoria Mattioni Marchetti Vittoria Mattioni Marchetti Jaroslav Hrabak Jaroslav Hrabak Costas C. Papagiannitsis Costas C. Papagiannitsis Costas C. Papagiannitsis Ibrahim Bitar Ibrahim Bitar Genetic Plurality of OXA/NDM-Encoding Features Characterized From Enterobacterales Recovered From Czech Hospitals Frontiers in Microbiology OXA-244 OXA-181 NDM-1 NDM-5 mobile genetic elements |
| title | Genetic Plurality of OXA/NDM-Encoding Features Characterized From Enterobacterales Recovered From Czech Hospitals |
| title_full | Genetic Plurality of OXA/NDM-Encoding Features Characterized From Enterobacterales Recovered From Czech Hospitals |
| title_fullStr | Genetic Plurality of OXA/NDM-Encoding Features Characterized From Enterobacterales Recovered From Czech Hospitals |
| title_full_unstemmed | Genetic Plurality of OXA/NDM-Encoding Features Characterized From Enterobacterales Recovered From Czech Hospitals |
| title_short | Genetic Plurality of OXA/NDM-Encoding Features Characterized From Enterobacterales Recovered From Czech Hospitals |
| title_sort | genetic plurality of oxa ndm encoding features characterized from enterobacterales recovered from czech hospitals |
| topic | OXA-244 OXA-181 NDM-1 NDM-5 mobile genetic elements |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.641415/full |
| work_keys_str_mv | AT katerinachudejova geneticpluralityofoxandmencodingfeaturescharacterizedfromenterobacteralesrecoveredfromczechhospitals AT katerinachudejova geneticpluralityofoxandmencodingfeaturescharacterizedfromenterobacteralesrecoveredfromczechhospitals AT luciekraftova geneticpluralityofoxandmencodingfeaturescharacterizedfromenterobacteralesrecoveredfromczechhospitals AT luciekraftova geneticpluralityofoxandmencodingfeaturescharacterizedfromenterobacteralesrecoveredfromczechhospitals AT vittoriamattionimarchetti geneticpluralityofoxandmencodingfeaturescharacterizedfromenterobacteralesrecoveredfromczechhospitals AT vittoriamattionimarchetti geneticpluralityofoxandmencodingfeaturescharacterizedfromenterobacteralesrecoveredfromczechhospitals AT jaroslavhrabak geneticpluralityofoxandmencodingfeaturescharacterizedfromenterobacteralesrecoveredfromczechhospitals AT jaroslavhrabak geneticpluralityofoxandmencodingfeaturescharacterizedfromenterobacteralesrecoveredfromczechhospitals AT costascpapagiannitsis geneticpluralityofoxandmencodingfeaturescharacterizedfromenterobacteralesrecoveredfromczechhospitals AT costascpapagiannitsis geneticpluralityofoxandmencodingfeaturescharacterizedfromenterobacteralesrecoveredfromczechhospitals AT costascpapagiannitsis geneticpluralityofoxandmencodingfeaturescharacterizedfromenterobacteralesrecoveredfromczechhospitals AT ibrahimbitar geneticpluralityofoxandmencodingfeaturescharacterizedfromenterobacteralesrecoveredfromczechhospitals AT ibrahimbitar geneticpluralityofoxandmencodingfeaturescharacterizedfromenterobacteralesrecoveredfromczechhospitals |