Detection of New <i>Leptospira</i> Genotypes Infecting Symptomatic Dogs: Is a New Vaccine Formulation Needed?
Leptospirosis in dogs has been largely described worldwide, and epidemiological studies have been mainly based on serological data. This study aims to detect and genotype leptospires affecting symptomatic dogs in Northeast Italy between 2013 and 2019. Overall, 1631 dogs were tested using real-time P...
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MDPI AG
2020-06-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/6/484 |
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| author | Cristina Bertasio Maria Beatrice Boniotti Laura Lucchese Letizia Ceglie Laura Bellinati Matteo Mazzucato Tommaso Furlanello Mario D’Incau Alda Natale |
| author_facet | Cristina Bertasio Maria Beatrice Boniotti Laura Lucchese Letizia Ceglie Laura Bellinati Matteo Mazzucato Tommaso Furlanello Mario D’Incau Alda Natale |
| author_sort | Cristina Bertasio |
| collection | DOAJ |
| description | Leptospirosis in dogs has been largely described worldwide, and epidemiological studies have been mainly based on serological data. This study aims to detect and genotype leptospires affecting symptomatic dogs in Northeast Italy between 2013 and 2019. Overall, 1631 dogs were tested using real-time PCR, and leptospires from 193 dogs were subjected to Multilocus Sequence Typing and a Multiple Loci Variable-number Tandem Repeat Analysis. Leptospires were successfully isolated from 15 symptomatic dogs. Six distinct Sequence Types (STs) were found for 135 leptospires, with 3 STs characterizing <i>Leptospira interrogans</i> (ST17, ST198 and ST24), 2 STs characterizing <i>Leptospira kirschneri</i> (ST117 and ST289) and 1 ST characterizing <i>Leptospira borgpetersenii</i> (ST155), revealing the circulation of the serogroups Icterohaemorrhagiae, Australis, Sejroe and Pomona. The Multiple Loci Variable-number Tandem Repeat Analysis of 17 samples did not result in any additional discrimination. Genotypes were compared with those of strains present in the historical internal database, and possible transmission chains were identified from rat, mouse, hedgehog and pig. This work highlights the importance of molecular methods in revealing and identifying circulating <i>Leptospira</i> strains, and it also encourages the evaluation of the ability of commercially available vaccines to reduce the disease burden among dogs. |
| first_indexed | 2024-03-10T19:04:10Z |
| format | Article |
| id | doaj.art-67346e9ac420470c9edd798567a53040 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T19:04:10Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-67346e9ac420470c9edd798567a530402023-11-20T04:13:44ZengMDPI AGPathogens2076-08172020-06-019648410.3390/pathogens9060484Detection of New <i>Leptospira</i> Genotypes Infecting Symptomatic Dogs: Is a New Vaccine Formulation Needed?Cristina Bertasio0Maria Beatrice Boniotti1Laura Lucchese2Letizia Ceglie3Laura Bellinati4Matteo Mazzucato5Tommaso Furlanello6Mario D’Incau7Alda Natale8National Reference Centre for Animal Leptospirosis, Istituto Zooprofilattico Sperimentale della Lombardia ed Emilia Romagna “Bruno Ubertini”, 25121 Brescia, ItalyNational Reference Centre for Animal Leptospirosis, Istituto Zooprofilattico Sperimentale della Lombardia ed Emilia Romagna “Bruno Ubertini”, 25121 Brescia, ItalyIstituto Zooprofilattico Sperimentale delle Venezie, Viale dell’Università, 35020 Legnaro, ItalyIstituto Zooprofilattico Sperimentale delle Venezie, Viale dell’Università, 35020 Legnaro, ItalyIstituto Zooprofilattico Sperimentale delle Venezie, Viale dell’Università, 35020 Legnaro, ItalyIstituto Zooprofilattico Sperimentale delle Venezie, Viale dell’Università, 35020 Legnaro, Italy“San Marco” Veterinary Clinica and Laboratory, Via dell’ Industria, 35030 Veggiano, ItalyNational Reference Centre for Animal Leptospirosis, Istituto Zooprofilattico Sperimentale della Lombardia ed Emilia Romagna “Bruno Ubertini”, 25121 Brescia, ItalyIstituto Zooprofilattico Sperimentale delle Venezie, Viale dell’Università, 35020 Legnaro, ItalyLeptospirosis in dogs has been largely described worldwide, and epidemiological studies have been mainly based on serological data. This study aims to detect and genotype leptospires affecting symptomatic dogs in Northeast Italy between 2013 and 2019. Overall, 1631 dogs were tested using real-time PCR, and leptospires from 193 dogs were subjected to Multilocus Sequence Typing and a Multiple Loci Variable-number Tandem Repeat Analysis. Leptospires were successfully isolated from 15 symptomatic dogs. Six distinct Sequence Types (STs) were found for 135 leptospires, with 3 STs characterizing <i>Leptospira interrogans</i> (ST17, ST198 and ST24), 2 STs characterizing <i>Leptospira kirschneri</i> (ST117 and ST289) and 1 ST characterizing <i>Leptospira borgpetersenii</i> (ST155), revealing the circulation of the serogroups Icterohaemorrhagiae, Australis, Sejroe and Pomona. The Multiple Loci Variable-number Tandem Repeat Analysis of 17 samples did not result in any additional discrimination. Genotypes were compared with those of strains present in the historical internal database, and possible transmission chains were identified from rat, mouse, hedgehog and pig. This work highlights the importance of molecular methods in revealing and identifying circulating <i>Leptospira</i> strains, and it also encourages the evaluation of the ability of commercially available vaccines to reduce the disease burden among dogs.https://www.mdpi.com/2076-0817/9/6/484leptospirosisdogreal-time PCRgenotypingepidemiologymultilocus sequence typing |
| spellingShingle | Cristina Bertasio Maria Beatrice Boniotti Laura Lucchese Letizia Ceglie Laura Bellinati Matteo Mazzucato Tommaso Furlanello Mario D’Incau Alda Natale Detection of New <i>Leptospira</i> Genotypes Infecting Symptomatic Dogs: Is a New Vaccine Formulation Needed? Pathogens leptospirosis dog real-time PCR genotyping epidemiology multilocus sequence typing |
| title | Detection of New <i>Leptospira</i> Genotypes Infecting Symptomatic Dogs: Is a New Vaccine Formulation Needed? |
| title_full | Detection of New <i>Leptospira</i> Genotypes Infecting Symptomatic Dogs: Is a New Vaccine Formulation Needed? |
| title_fullStr | Detection of New <i>Leptospira</i> Genotypes Infecting Symptomatic Dogs: Is a New Vaccine Formulation Needed? |
| title_full_unstemmed | Detection of New <i>Leptospira</i> Genotypes Infecting Symptomatic Dogs: Is a New Vaccine Formulation Needed? |
| title_short | Detection of New <i>Leptospira</i> Genotypes Infecting Symptomatic Dogs: Is a New Vaccine Formulation Needed? |
| title_sort | detection of new i leptospira i genotypes infecting symptomatic dogs is a new vaccine formulation needed |
| topic | leptospirosis dog real-time PCR genotyping epidemiology multilocus sequence typing |
| url | https://www.mdpi.com/2076-0817/9/6/484 |
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