Assessment of the Prevalence and Drug Susceptibility of <i>Listeria monocytogenes</i> Strains Isolated from Various Types of Meat
<i>Listeria monocytogenes</i> are the etiological factor of listeriosis, and their main source for humans is food. The aim of the current study was to assess the contamination of various types of meat and the drug susceptibility of isolated <i>L. monocytogenes</i>. Between 20...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/9/9/1293 |
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| author | Krzysztof Skowron Ewa Wałecka-Zacharska Natalia Wiktorczyk-Kapischke Karolina Jadwiga Skowron Katarzyna Grudlewska-Buda Justyna Bauza-Kaszewska Zuzanna Bernaciak Miłosz Borkowski Eugenia Gospodarek-Komkowska |
| author_facet | Krzysztof Skowron Ewa Wałecka-Zacharska Natalia Wiktorczyk-Kapischke Karolina Jadwiga Skowron Katarzyna Grudlewska-Buda Justyna Bauza-Kaszewska Zuzanna Bernaciak Miłosz Borkowski Eugenia Gospodarek-Komkowska |
| author_sort | Krzysztof Skowron |
| collection | DOAJ |
| description | <i>Listeria monocytogenes</i> are the etiological factor of listeriosis, and their main source for humans is food. The aim of the current study was to assess the contamination of various types of meat and the drug susceptibility of isolated <i>L. monocytogenes</i>. Between 2016–2018, 6000 swabs were taken (2000 annually) from the surface of pork, beef, and poultry. The analysis of intermediate and finished product samples was carried out in accordance with ISO 11290-1 (International Organization for Standardization). The genetic similarity assessment of the isolates obtained was based on the Pulsed Field Gel Electrophoresis (PFGE) method, and drug-sensitivity assessment using the disc-diffusion method. We found 2.1% of collected samples were <i>L. monocytogenes</i> positive. The level of meat contamination varied depending on its matrix. Most <i>L. monocytogenes</i> were isolated from poultry. It was shown that 39 (32.5%) strains were sensitive to all tested antibiotics and eight (6.7%) were resistant to all five tested antimicrobials. Most strains tested were resistant to cotrimoxazole (55; 45.8%) and meropenem (52; 43.3%), followed by erythromycin (48; 40.0%), penicillin (31; 25.8%), and ampicillin (21; 17.5%). High prevalence of this pathogen may be a serious problem, especially when linked with antibiotic resistance and high percentage of serotypes responsible for listeriosis outbreaks. |
| first_indexed | 2024-03-10T16:20:35Z |
| format | Article |
| id | doaj.art-67350800c8ef43488ae158e312df8066 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T16:20:35Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-67350800c8ef43488ae158e312df80662023-11-20T13:43:37ZengMDPI AGFoods2304-81582020-09-0199129310.3390/foods9091293Assessment of the Prevalence and Drug Susceptibility of <i>Listeria monocytogenes</i> Strains Isolated from Various Types of MeatKrzysztof Skowron0Ewa Wałecka-Zacharska1Natalia Wiktorczyk-Kapischke2Karolina Jadwiga Skowron3Katarzyna Grudlewska-Buda4Justyna Bauza-Kaszewska5Zuzanna Bernaciak6Miłosz Borkowski7Eugenia Gospodarek-Komkowska8Department of Microbiology, Nicolaus Copernicus University in Toruń, Ludwik Rydygier Collegium Medicum, 9 M. Skłodowskiej-Curie Street, 85-094 Bydgoszcz, PolandDepartment of Food Hygiene and Consumer Health, Wrocław University of Environmental and Life Sciences, 31 C.K. Norwida St., 50-375 Wrocław, PolandDepartment of Microbiology, Nicolaus Copernicus University in Toruń, Ludwik Rydygier Collegium Medicum, 9 M. Skłodowskiej-Curie Street, 85-094 Bydgoszcz, PolandInstitute of Telecommunications and Computer Science, UTP University of Science and Technology, Al. Prof. S. Kaliskiego 7, 85-796 Bydgoszcz, PolandDepartment of Microbiology, Nicolaus Copernicus University in Toruń, Ludwik Rydygier Collegium Medicum, 9 M. Skłodowskiej-Curie Street, 85-094 Bydgoszcz, PolandDepartment of Microbiology and Food Technology, UTP University of Science and Technology, 6 Bernardyńska St., 85-029 Bydgoszcz, PolandDepartment of Microbiology, Nicolaus Copernicus University in Toruń, Ludwik Rydygier Collegium Medicum, 9 M. Skłodowskiej-Curie Street, 85-094 Bydgoszcz, PolandDepartment of Microbiology, Nicolaus Copernicus University in Toruń, Ludwik Rydygier Collegium Medicum, 9 M. Skłodowskiej-Curie Street, 85-094 Bydgoszcz, PolandDepartment of Microbiology, Nicolaus Copernicus University in Toruń, Ludwik Rydygier Collegium Medicum, 9 M. Skłodowskiej-Curie Street, 85-094 Bydgoszcz, Poland<i>Listeria monocytogenes</i> are the etiological factor of listeriosis, and their main source for humans is food. The aim of the current study was to assess the contamination of various types of meat and the drug susceptibility of isolated <i>L. monocytogenes</i>. Between 2016–2018, 6000 swabs were taken (2000 annually) from the surface of pork, beef, and poultry. The analysis of intermediate and finished product samples was carried out in accordance with ISO 11290-1 (International Organization for Standardization). The genetic similarity assessment of the isolates obtained was based on the Pulsed Field Gel Electrophoresis (PFGE) method, and drug-sensitivity assessment using the disc-diffusion method. We found 2.1% of collected samples were <i>L. monocytogenes</i> positive. The level of meat contamination varied depending on its matrix. Most <i>L. monocytogenes</i> were isolated from poultry. It was shown that 39 (32.5%) strains were sensitive to all tested antibiotics and eight (6.7%) were resistant to all five tested antimicrobials. Most strains tested were resistant to cotrimoxazole (55; 45.8%) and meropenem (52; 43.3%), followed by erythromycin (48; 40.0%), penicillin (31; 25.8%), and ampicillin (21; 17.5%). High prevalence of this pathogen may be a serious problem, especially when linked with antibiotic resistance and high percentage of serotypes responsible for listeriosis outbreaks.https://www.mdpi.com/2304-8158/9/9/1293<i>Listeria monocytogenes</i>meatlisteriosisdrug susceptibilityantibiotics |
| spellingShingle | Krzysztof Skowron Ewa Wałecka-Zacharska Natalia Wiktorczyk-Kapischke Karolina Jadwiga Skowron Katarzyna Grudlewska-Buda Justyna Bauza-Kaszewska Zuzanna Bernaciak Miłosz Borkowski Eugenia Gospodarek-Komkowska Assessment of the Prevalence and Drug Susceptibility of <i>Listeria monocytogenes</i> Strains Isolated from Various Types of Meat Foods <i>Listeria monocytogenes</i> meat listeriosis drug susceptibility antibiotics |
| title | Assessment of the Prevalence and Drug Susceptibility of <i>Listeria monocytogenes</i> Strains Isolated from Various Types of Meat |
| title_full | Assessment of the Prevalence and Drug Susceptibility of <i>Listeria monocytogenes</i> Strains Isolated from Various Types of Meat |
| title_fullStr | Assessment of the Prevalence and Drug Susceptibility of <i>Listeria monocytogenes</i> Strains Isolated from Various Types of Meat |
| title_full_unstemmed | Assessment of the Prevalence and Drug Susceptibility of <i>Listeria monocytogenes</i> Strains Isolated from Various Types of Meat |
| title_short | Assessment of the Prevalence and Drug Susceptibility of <i>Listeria monocytogenes</i> Strains Isolated from Various Types of Meat |
| title_sort | assessment of the prevalence and drug susceptibility of i listeria monocytogenes i strains isolated from various types of meat |
| topic | <i>Listeria monocytogenes</i> meat listeriosis drug susceptibility antibiotics |
| url | https://www.mdpi.com/2304-8158/9/9/1293 |
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