Genetic Variation for Cold Tolerance in Two Nested Association Mapping Populations
Cold reduces maize (<i>Zea mays</i> L.) production and delays sowings. Cold tolerance in maize is very limited, and breeding maize for cold tolerance is still a major challenge. Our objective was to detect QTL for cold tolerance at germination and seedling stages. We evaluated, under col...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/1/195 |
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| author | Pedro Revilla Ana Butrón Víctor Manuel Rodriguez Renaud Rincent Alain Charcosset Catherine Giauffret Albrecht E. Melchinger Chris-Carolin Schön Eva Bauer Thomas Altmann Dominique Brunel Jesús Moreno-González Laura Campo Milena Ouzunova Ángel Álvarez José Ignacio Ruíz de Galarreta Jacques Laborde Rosa Ana Malvar |
| author_facet | Pedro Revilla Ana Butrón Víctor Manuel Rodriguez Renaud Rincent Alain Charcosset Catherine Giauffret Albrecht E. Melchinger Chris-Carolin Schön Eva Bauer Thomas Altmann Dominique Brunel Jesús Moreno-González Laura Campo Milena Ouzunova Ángel Álvarez José Ignacio Ruíz de Galarreta Jacques Laborde Rosa Ana Malvar |
| author_sort | Pedro Revilla |
| collection | DOAJ |
| description | Cold reduces maize (<i>Zea mays</i> L.) production and delays sowings. Cold tolerance in maize is very limited, and breeding maize for cold tolerance is still a major challenge. Our objective was to detect QTL for cold tolerance at germination and seedling stages. We evaluated, under cold and control conditions, 919 Dent and 1009 Flint inbred lines from two nested association mapping designs consisting in 24 double-haploid populations, genotyped with 56,110 SNPs. We found a large diversity of maize cold tolerance within these NAM populations. We detected one QTL for plant weight and four for fluorescence under cold conditions, as well as one for plant weight and two for chlorophyll content under control conditions in the Dent-NAM. There were fewer significant QTL under control conditions than under cold conditions, and half of the QTL were for quantum efficiency of photosystem II. Our results supported the large genetic discrepancy between optimal and low temperatures, as the quantity and the position of the QTL were very variable between control and cold conditions. Furthermore, as we have not found alleles with significant effects on these NAM designs, further studies are needed with other experimental designs to find favorable alleles with important effects for improving cold tolerance in maize. |
| first_indexed | 2024-03-09T13:51:06Z |
| format | Article |
| id | doaj.art-6738fd67c73f4d0d90e7b4c981929fa9 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T13:51:06Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-6738fd67c73f4d0d90e7b4c981929fa92023-11-30T20:50:17ZengMDPI AGAgronomy2073-43952023-01-0113119510.3390/agronomy13010195Genetic Variation for Cold Tolerance in Two Nested Association Mapping PopulationsPedro Revilla0Ana Butrón1Víctor Manuel Rodriguez2Renaud Rincent3Alain Charcosset4Catherine Giauffret5Albrecht E. Melchinger6Chris-Carolin Schön7Eva Bauer8Thomas Altmann9Dominique Brunel10Jesús Moreno-González11Laura Campo12Milena Ouzunova13Ángel Álvarez14José Ignacio Ruíz de Galarreta15Jacques Laborde16Rosa Ana Malvar17Misión Biológica de Galicia (CSIC), Apartado 28, 36080 Pontevedra, SpainMisión Biológica de Galicia (CSIC), Apartado 28, 36080 Pontevedra, SpainMisión Biológica de Galicia (CSIC), Apartado 28, 36080 Pontevedra, SpainINRA, UMR de Génétique Végétale, Université Paris-Sud–CNRS–AgroParisTech, 91190 Gif-sur-Yvette, FranceINRA, UMR de Génétique Végétale, Université Paris-Sud–CNRS–AgroParisTech, 91190 Gif-sur-Yvette, FranceUnité Mixte de Recherche, Institu National de la Recherche Agronomique, University of Science and Technology, 1281, Stress Abiotiques et Différenciation des Végetaux Cultivés, 59655 Péronne, FranceInstitute of Plant Breeding, Seed Science and Population Genetics, Universität Hohenheim, 70599 Stuttgart, GermanyPlant Breeding, Technische Universität München, 85354 Freising, GermanyPlant Breeding, Technische Universität München, 85354 Freising, GermanyMolecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466 Gatersleben, GermanyINRA-VERSAILLES, 91057 Evry, FranceCentro Investigacións Agrarias Mabegondo (CIAM), 15318 A Coruña, SpainCentro Investigacións Agrarias Mabegondo (CIAM), 15318 A Coruña, SpainKWS SAAT AG, 37574 Einbeck, GermanyEstación Experimental de Aula Dei (CSIC), 50059 Saragossa, SpainNEIKER-Instituto Vasco de Investigación y Desarrollo Agrario, 01192 Vitoria, SpainINRA, Stn Expt Mais, 40590 St Martin De Hinx, FranceMisión Biológica de Galicia (CSIC), Apartado 28, 36080 Pontevedra, SpainCold reduces maize (<i>Zea mays</i> L.) production and delays sowings. Cold tolerance in maize is very limited, and breeding maize for cold tolerance is still a major challenge. Our objective was to detect QTL for cold tolerance at germination and seedling stages. We evaluated, under cold and control conditions, 919 Dent and 1009 Flint inbred lines from two nested association mapping designs consisting in 24 double-haploid populations, genotyped with 56,110 SNPs. We found a large diversity of maize cold tolerance within these NAM populations. We detected one QTL for plant weight and four for fluorescence under cold conditions, as well as one for plant weight and two for chlorophyll content under control conditions in the Dent-NAM. There were fewer significant QTL under control conditions than under cold conditions, and half of the QTL were for quantum efficiency of photosystem II. Our results supported the large genetic discrepancy between optimal and low temperatures, as the quantity and the position of the QTL were very variable between control and cold conditions. Furthermore, as we have not found alleles with significant effects on these NAM designs, further studies are needed with other experimental designs to find favorable alleles with important effects for improving cold tolerance in maize.https://www.mdpi.com/2073-4395/13/1/195cold tolerancemaizeQTLNAMRIL |
| spellingShingle | Pedro Revilla Ana Butrón Víctor Manuel Rodriguez Renaud Rincent Alain Charcosset Catherine Giauffret Albrecht E. Melchinger Chris-Carolin Schön Eva Bauer Thomas Altmann Dominique Brunel Jesús Moreno-González Laura Campo Milena Ouzunova Ángel Álvarez José Ignacio Ruíz de Galarreta Jacques Laborde Rosa Ana Malvar Genetic Variation for Cold Tolerance in Two Nested Association Mapping Populations Agronomy cold tolerance maize QTL NAM RIL |
| title | Genetic Variation for Cold Tolerance in Two Nested Association Mapping Populations |
| title_full | Genetic Variation for Cold Tolerance in Two Nested Association Mapping Populations |
| title_fullStr | Genetic Variation for Cold Tolerance in Two Nested Association Mapping Populations |
| title_full_unstemmed | Genetic Variation for Cold Tolerance in Two Nested Association Mapping Populations |
| title_short | Genetic Variation for Cold Tolerance in Two Nested Association Mapping Populations |
| title_sort | genetic variation for cold tolerance in two nested association mapping populations |
| topic | cold tolerance maize QTL NAM RIL |
| url | https://www.mdpi.com/2073-4395/13/1/195 |
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