Quercetin Attenuates Endoplasmic Reticulum Stress and Apoptosis in TNBS-Induced Colitis by Inhibiting the Glucose Regulatory Protein 78 Activation
Background:The inflammatory bowel diseases (IBD) are significantly influenced by apoptosis and endoplasmic reticulum (ER) stress.Aims:To investigate the effects of quercetin on ER stress-mediated apoptosis in a trinitrobenzene sulfonic acid (TNBS) induced experimental IBD model.Study Design:In vivo...
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Galenos Publishing House
2024-01-01
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Series: | Balkan Medical Journal |
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http://balkanmedicaljournal.org/text.php?lang=en&id=2565
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author | Yeter Topçu-Tarladaçalışır Melike Sapmaz-Metin Zeynep Mercan Deniz Erçetin |
author_facet | Yeter Topçu-Tarladaçalışır Melike Sapmaz-Metin Zeynep Mercan Deniz Erçetin |
author_sort | Yeter Topçu-Tarladaçalışır |
collection | DOAJ |
description | Background:The inflammatory bowel diseases (IBD) are significantly influenced by apoptosis and endoplasmic reticulum (ER) stress.Aims:To investigate the effects of quercetin on ER stress-mediated apoptosis in a trinitrobenzene sulfonic acid (TNBS) induced experimental IBD model.Study Design:In vivo animal experimental study.Methods:To demonstrate the effect of quercetin in an experimental colitis model, Control, TNBS, and TNBS+quercetin groups were created with 24 Wistar Albino rats. Colitis was induced by intrarectal administration of 25 mg TNBS. In the TNBS+quercetin group, intragastrically 100 mg/kg quercetin was given for 7 days, immediately after colitis induction. In the TNBS-induced experimental IBD model, we evaluated the effects of quercetin on colonic epithelial cell apoptosis, oxidative stress, ER stress, the mitogen-activated protein kinase c-Jun N-terminal kinase, and the nuclear factor kappa B immunoreactivities, the levels of myeloperoxidase and tumor necrosis factor-α, the disease activity index with colonic histopathologic changes.Results:TNBS administration induced an elevated level of disease activity and oxidative stress indices, inflammation markers, and an increase in the immunoreactivities of nuclear factor kappa B and the mitogen-activated protein kinase c-Jun N-terminal kinase in the colon of the colitis group. Glucose regulatory protein 78, caspase-12 immunoreactivities, and epithelial cell apoptosis also were shown in the colon. However, quercetin improved TNBS-induced histopathological alterations, apoptosis, inflammation, oxidative stress, and ER stress.Conclusion:This study suggests that quercetin has a regulatory effect on ER stress-mediated apoptosis, and thus may be beneficial in treating IBD. |
first_indexed | 2024-03-08T15:49:47Z |
format | Article |
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language | English |
last_indexed | 2024-03-08T15:49:47Z |
publishDate | 2024-01-01 |
publisher | Galenos Publishing House |
record_format | Article |
series | Balkan Medical Journal |
spelling | doaj.art-673e835cd8674d7caa71540ac563ec692024-01-09T06:36:24ZengGalenos Publishing HouseBalkan Medical Journal2146-31232146-31312024-01-01411303710.4274/balkanmedj.galenos.2023.2023-10-913049054Quercetin Attenuates Endoplasmic Reticulum Stress and Apoptosis in TNBS-Induced Colitis by Inhibiting the Glucose Regulatory Protein 78 ActivationYeter Topçu-Tarladaçalışır0Melike Sapmaz-Metin1Zeynep Mercan2Deniz Erçetin3 Department of Histology and Embryology Trakya University Faculty of Medicine, Edirne, Türkiye Department of Histology and Embryology Trakya University Faculty of Medicine, Edirne, Türkiye Department of Histology and Embryology Gaziantep University Faculty of Medicine, Gaziantep, Türkiye Department of Histology and Embryology Trakya University Faculty of Medicine, Edirne, Türkiye Background:The inflammatory bowel diseases (IBD) are significantly influenced by apoptosis and endoplasmic reticulum (ER) stress.Aims:To investigate the effects of quercetin on ER stress-mediated apoptosis in a trinitrobenzene sulfonic acid (TNBS) induced experimental IBD model.Study Design:In vivo animal experimental study.Methods:To demonstrate the effect of quercetin in an experimental colitis model, Control, TNBS, and TNBS+quercetin groups were created with 24 Wistar Albino rats. Colitis was induced by intrarectal administration of 25 mg TNBS. In the TNBS+quercetin group, intragastrically 100 mg/kg quercetin was given for 7 days, immediately after colitis induction. In the TNBS-induced experimental IBD model, we evaluated the effects of quercetin on colonic epithelial cell apoptosis, oxidative stress, ER stress, the mitogen-activated protein kinase c-Jun N-terminal kinase, and the nuclear factor kappa B immunoreactivities, the levels of myeloperoxidase and tumor necrosis factor-α, the disease activity index with colonic histopathologic changes.Results:TNBS administration induced an elevated level of disease activity and oxidative stress indices, inflammation markers, and an increase in the immunoreactivities of nuclear factor kappa B and the mitogen-activated protein kinase c-Jun N-terminal kinase in the colon of the colitis group. Glucose regulatory protein 78, caspase-12 immunoreactivities, and epithelial cell apoptosis also were shown in the colon. However, quercetin improved TNBS-induced histopathological alterations, apoptosis, inflammation, oxidative stress, and ER stress.Conclusion:This study suggests that quercetin has a regulatory effect on ER stress-mediated apoptosis, and thus may be beneficial in treating IBD. http://balkanmedicaljournal.org/text.php?lang=en&id=2565 |
spellingShingle | Yeter Topçu-Tarladaçalışır Melike Sapmaz-Metin Zeynep Mercan Deniz Erçetin Quercetin Attenuates Endoplasmic Reticulum Stress and Apoptosis in TNBS-Induced Colitis by Inhibiting the Glucose Regulatory Protein 78 Activation Balkan Medical Journal |
title | Quercetin Attenuates Endoplasmic Reticulum Stress and Apoptosis in TNBS-Induced Colitis by Inhibiting the Glucose Regulatory Protein 78 Activation |
title_full | Quercetin Attenuates Endoplasmic Reticulum Stress and Apoptosis in TNBS-Induced Colitis by Inhibiting the Glucose Regulatory Protein 78 Activation |
title_fullStr | Quercetin Attenuates Endoplasmic Reticulum Stress and Apoptosis in TNBS-Induced Colitis by Inhibiting the Glucose Regulatory Protein 78 Activation |
title_full_unstemmed | Quercetin Attenuates Endoplasmic Reticulum Stress and Apoptosis in TNBS-Induced Colitis by Inhibiting the Glucose Regulatory Protein 78 Activation |
title_short | Quercetin Attenuates Endoplasmic Reticulum Stress and Apoptosis in TNBS-Induced Colitis by Inhibiting the Glucose Regulatory Protein 78 Activation |
title_sort | quercetin attenuates endoplasmic reticulum stress and apoptosis in tnbs induced colitis by inhibiting the glucose regulatory protein 78 activation |
url |
http://balkanmedicaljournal.org/text.php?lang=en&id=2565
|
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