Quercetin Attenuates Endoplasmic Reticulum Stress and Apoptosis in TNBS-Induced Colitis by Inhibiting the Glucose Regulatory Protein 78 Activation

Background:The inflammatory bowel diseases (IBD) are significantly influenced by apoptosis and endoplasmic reticulum (ER) stress.Aims:To investigate the effects of quercetin on ER stress-mediated apoptosis in a trinitrobenzene sulfonic acid (TNBS) induced experimental IBD model.Study Design:In vivo...

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Main Authors: Yeter Topçu-Tarladaçalışır, Melike Sapmaz-Metin, Zeynep Mercan, Deniz Erçetin
Format: Article
Language:English
Published: Galenos Publishing House 2024-01-01
Series:Balkan Medical Journal
Online Access: http://balkanmedicaljournal.org/text.php?lang=en&id=2565
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author Yeter Topçu-Tarladaçalışır
Melike Sapmaz-Metin
Zeynep Mercan
Deniz Erçetin
author_facet Yeter Topçu-Tarladaçalışır
Melike Sapmaz-Metin
Zeynep Mercan
Deniz Erçetin
author_sort Yeter Topçu-Tarladaçalışır
collection DOAJ
description Background:The inflammatory bowel diseases (IBD) are significantly influenced by apoptosis and endoplasmic reticulum (ER) stress.Aims:To investigate the effects of quercetin on ER stress-mediated apoptosis in a trinitrobenzene sulfonic acid (TNBS) induced experimental IBD model.Study Design:In vivo animal experimental study.Methods:To demonstrate the effect of quercetin in an experimental colitis model, Control, TNBS, and TNBS+quercetin groups were created with 24 Wistar Albino rats. Colitis was induced by intrarectal administration of 25 mg TNBS. In the TNBS+quercetin group, intragastrically 100 mg/kg quercetin was given for 7 days, immediately after colitis induction. In the TNBS-induced experimental IBD model, we evaluated the effects of quercetin on colonic epithelial cell apoptosis, oxidative stress, ER stress, the mitogen-activated protein kinase c-Jun N-terminal kinase, and the nuclear factor kappa B immunoreactivities, the levels of myeloperoxidase and tumor necrosis factor-α, the disease activity index with colonic histopathologic changes.Results:TNBS administration induced an elevated level of disease activity and oxidative stress indices, inflammation markers, and an increase in the immunoreactivities of nuclear factor kappa B and the mitogen-activated protein kinase c-Jun N-terminal kinase in the colon of the colitis group. Glucose regulatory protein 78, caspase-12 immunoreactivities, and epithelial cell apoptosis also were shown in the colon. However, quercetin improved TNBS-induced histopathological alterations, apoptosis, inflammation, oxidative stress, and ER stress.Conclusion:This study suggests that quercetin has a regulatory effect on ER stress-mediated apoptosis, and thus may be beneficial in treating IBD.
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spelling doaj.art-673e835cd8674d7caa71540ac563ec692024-01-09T06:36:24ZengGalenos Publishing HouseBalkan Medical Journal2146-31232146-31312024-01-01411303710.4274/balkanmedj.galenos.2023.2023-10-913049054Quercetin Attenuates Endoplasmic Reticulum Stress and Apoptosis in TNBS-Induced Colitis by Inhibiting the Glucose Regulatory Protein 78 ActivationYeter Topçu-Tarladaçalışır0Melike Sapmaz-Metin1Zeynep Mercan2Deniz Erçetin3 Department of Histology and Embryology Trakya University Faculty of Medicine, Edirne, Türkiye Department of Histology and Embryology Trakya University Faculty of Medicine, Edirne, Türkiye Department of Histology and Embryology Gaziantep University Faculty of Medicine, Gaziantep, Türkiye Department of Histology and Embryology Trakya University Faculty of Medicine, Edirne, Türkiye Background:The inflammatory bowel diseases (IBD) are significantly influenced by apoptosis and endoplasmic reticulum (ER) stress.Aims:To investigate the effects of quercetin on ER stress-mediated apoptosis in a trinitrobenzene sulfonic acid (TNBS) induced experimental IBD model.Study Design:In vivo animal experimental study.Methods:To demonstrate the effect of quercetin in an experimental colitis model, Control, TNBS, and TNBS+quercetin groups were created with 24 Wistar Albino rats. Colitis was induced by intrarectal administration of 25 mg TNBS. In the TNBS+quercetin group, intragastrically 100 mg/kg quercetin was given for 7 days, immediately after colitis induction. In the TNBS-induced experimental IBD model, we evaluated the effects of quercetin on colonic epithelial cell apoptosis, oxidative stress, ER stress, the mitogen-activated protein kinase c-Jun N-terminal kinase, and the nuclear factor kappa B immunoreactivities, the levels of myeloperoxidase and tumor necrosis factor-α, the disease activity index with colonic histopathologic changes.Results:TNBS administration induced an elevated level of disease activity and oxidative stress indices, inflammation markers, and an increase in the immunoreactivities of nuclear factor kappa B and the mitogen-activated protein kinase c-Jun N-terminal kinase in the colon of the colitis group. Glucose regulatory protein 78, caspase-12 immunoreactivities, and epithelial cell apoptosis also were shown in the colon. However, quercetin improved TNBS-induced histopathological alterations, apoptosis, inflammation, oxidative stress, and ER stress.Conclusion:This study suggests that quercetin has a regulatory effect on ER stress-mediated apoptosis, and thus may be beneficial in treating IBD. http://balkanmedicaljournal.org/text.php?lang=en&id=2565
spellingShingle Yeter Topçu-Tarladaçalışır
Melike Sapmaz-Metin
Zeynep Mercan
Deniz Erçetin
Quercetin Attenuates Endoplasmic Reticulum Stress and Apoptosis in TNBS-Induced Colitis by Inhibiting the Glucose Regulatory Protein 78 Activation
Balkan Medical Journal
title Quercetin Attenuates Endoplasmic Reticulum Stress and Apoptosis in TNBS-Induced Colitis by Inhibiting the Glucose Regulatory Protein 78 Activation
title_full Quercetin Attenuates Endoplasmic Reticulum Stress and Apoptosis in TNBS-Induced Colitis by Inhibiting the Glucose Regulatory Protein 78 Activation
title_fullStr Quercetin Attenuates Endoplasmic Reticulum Stress and Apoptosis in TNBS-Induced Colitis by Inhibiting the Glucose Regulatory Protein 78 Activation
title_full_unstemmed Quercetin Attenuates Endoplasmic Reticulum Stress and Apoptosis in TNBS-Induced Colitis by Inhibiting the Glucose Regulatory Protein 78 Activation
title_short Quercetin Attenuates Endoplasmic Reticulum Stress and Apoptosis in TNBS-Induced Colitis by Inhibiting the Glucose Regulatory Protein 78 Activation
title_sort quercetin attenuates endoplasmic reticulum stress and apoptosis in tnbs induced colitis by inhibiting the glucose regulatory protein 78 activation
url http://balkanmedicaljournal.org/text.php?lang=en&id=2565
work_keys_str_mv AT yetertopcutarladacalısır quercetinattenuatesendoplasmicreticulumstressandapoptosisintnbsinducedcolitisbyinhibitingtheglucoseregulatoryprotein78activation
AT melikesapmazmetin quercetinattenuatesendoplasmicreticulumstressandapoptosisintnbsinducedcolitisbyinhibitingtheglucoseregulatoryprotein78activation
AT zeynepmercan quercetinattenuatesendoplasmicreticulumstressandapoptosisintnbsinducedcolitisbyinhibitingtheglucoseregulatoryprotein78activation
AT denizercetin quercetinattenuatesendoplasmicreticulumstressandapoptosisintnbsinducedcolitisbyinhibitingtheglucoseregulatoryprotein78activation