Effect of Chinese herbal formulae (BU-SHEN-YI-QI granule) treatment on thrombin expression after ischemia/reperfusion
Bu-Shen-Yi-Qi granule (BSYQG), a Chinese herbal formula, has been used for the treatment of ischemic stroke with proven efficacy. The purpose of this study is to evaluate the therapeutic efficacy of BSYQG in neurovascular unit (NVU) and its mechanism of action on the thrombin expression in the cereb...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | All Life |
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| Online Access: | http://dx.doi.org/10.1080/26895293.2023.2173311 |
| _version_ | 1797401351097942016 |
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| author | Aihua Liu Jing Sun Sagun Tiwari John Wong Honglin Wang Dongxu Tang Zhenxiang Han |
| author_facet | Aihua Liu Jing Sun Sagun Tiwari John Wong Honglin Wang Dongxu Tang Zhenxiang Han |
| author_sort | Aihua Liu |
| collection | DOAJ |
| description | Bu-Shen-Yi-Qi granule (BSYQG), a Chinese herbal formula, has been used for the treatment of ischemic stroke with proven efficacy. The purpose of this study is to evaluate the therapeutic efficacy of BSYQG in neurovascular unit (NVU) and its mechanism of action on the thrombin expression in the cerebral ischemic/reperfusion (I/R) model. In the study, a rat model of I/R was established using middle cerebral artery occlusion operation (MCAO). Adult male Sprague–Dawley rats were divided into four groups (n = 36): sham-operation; I/R; I/R + BSYQG(6 g/kg/day), I/R + Argatroban (0.56 mg/ml). Apoptotic cells in the NVU were detected by doubling-labeling using TUNEL assay and immunofluorescence. The dynamics of thrombin expression was detected using immunofluorescence on both sides after I/R injury. Compared with I/R group the numbers and rates of neuronal apoptosis in BSYQG groups significantly decreased (P < 0.05). Furthermore, in the ischemic cortex the thrombin expression at 4 h was significantly higher than that at 1 h (P < 0.01) and 2 h (P < 0.01) after I/R. BSYQG treatment significantly reduced thrombin expression both at 4 h (P < 0.001) and 6 h (P < 0.05) after I/R. Therefore, BSYQG may exert its protective effects in NVU after I/R injury by inhibiting thrombin expression. The results provided the foundation for further investigation in the treatment of ischemic stroke. |
| first_indexed | 2024-03-09T02:08:47Z |
| format | Article |
| id | doaj.art-673ec7a11f514d07857902169e4d9fb1 |
| institution | Directory Open Access Journal |
| issn | 2689-5307 |
| language | English |
| last_indexed | 2024-03-09T02:08:47Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | All Life |
| spelling | doaj.art-673ec7a11f514d07857902169e4d9fb12023-12-07T15:12:04ZengTaylor & Francis GroupAll Life2689-53072023-12-0116110.1080/26895293.2023.21733112173311Effect of Chinese herbal formulae (BU-SHEN-YI-QI granule) treatment on thrombin expression after ischemia/reperfusionAihua Liu0Jing Sun1Sagun Tiwari2John Wong3Honglin Wang4Dongxu Tang5Zhenxiang Han6Huashan Hospital Affiliated to Fudan UniversityHuashan Hospital Affiliated to Fudan UniversitySeventh People's Hospital of Shanghai University of TCMMGH Institute of Health ProfessionsSeventh People's Hospital of Shanghai University of TCMSeventh People's Hospital of Shanghai University of TCMSeventh People's Hospital of Shanghai University of TCMBu-Shen-Yi-Qi granule (BSYQG), a Chinese herbal formula, has been used for the treatment of ischemic stroke with proven efficacy. The purpose of this study is to evaluate the therapeutic efficacy of BSYQG in neurovascular unit (NVU) and its mechanism of action on the thrombin expression in the cerebral ischemic/reperfusion (I/R) model. In the study, a rat model of I/R was established using middle cerebral artery occlusion operation (MCAO). Adult male Sprague–Dawley rats were divided into four groups (n = 36): sham-operation; I/R; I/R + BSYQG(6 g/kg/day), I/R + Argatroban (0.56 mg/ml). Apoptotic cells in the NVU were detected by doubling-labeling using TUNEL assay and immunofluorescence. The dynamics of thrombin expression was detected using immunofluorescence on both sides after I/R injury. Compared with I/R group the numbers and rates of neuronal apoptosis in BSYQG groups significantly decreased (P < 0.05). Furthermore, in the ischemic cortex the thrombin expression at 4 h was significantly higher than that at 1 h (P < 0.01) and 2 h (P < 0.01) after I/R. BSYQG treatment significantly reduced thrombin expression both at 4 h (P < 0.001) and 6 h (P < 0.05) after I/R. Therefore, BSYQG may exert its protective effects in NVU after I/R injury by inhibiting thrombin expression. The results provided the foundation for further investigation in the treatment of ischemic stroke.http://dx.doi.org/10.1080/26895293.2023.2173311bu-shen-yi-qi granules (bsyqg)cerebral ischemia/reperfusion injuryneurovascular unit (nvu)thrombin expression |
| spellingShingle | Aihua Liu Jing Sun Sagun Tiwari John Wong Honglin Wang Dongxu Tang Zhenxiang Han Effect of Chinese herbal formulae (BU-SHEN-YI-QI granule) treatment on thrombin expression after ischemia/reperfusion All Life bu-shen-yi-qi granules (bsyqg) cerebral ischemia/reperfusion injury neurovascular unit (nvu) thrombin expression |
| title | Effect of Chinese herbal formulae (BU-SHEN-YI-QI granule) treatment on thrombin expression after ischemia/reperfusion |
| title_full | Effect of Chinese herbal formulae (BU-SHEN-YI-QI granule) treatment on thrombin expression after ischemia/reperfusion |
| title_fullStr | Effect of Chinese herbal formulae (BU-SHEN-YI-QI granule) treatment on thrombin expression after ischemia/reperfusion |
| title_full_unstemmed | Effect of Chinese herbal formulae (BU-SHEN-YI-QI granule) treatment on thrombin expression after ischemia/reperfusion |
| title_short | Effect of Chinese herbal formulae (BU-SHEN-YI-QI granule) treatment on thrombin expression after ischemia/reperfusion |
| title_sort | effect of chinese herbal formulae bu shen yi qi granule treatment on thrombin expression after ischemia reperfusion |
| topic | bu-shen-yi-qi granules (bsyqg) cerebral ischemia/reperfusion injury neurovascular unit (nvu) thrombin expression |
| url | http://dx.doi.org/10.1080/26895293.2023.2173311 |
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