Insights into Proteomics Reveal Mechanisms of Ethanol-Enhanced Bacterial Cellulose Biosynthesis by <i>Komagataeibacter nataicola</i>
Nata de coco, known as bacterial cellulose (BC), has been given much attention in the food industry and biomaterial areas due to its specific properties such as low calorie content, high content of fiber, high purity and high biocompatibility. <i>Komagataeibacter</i> spp. are indispensab...
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| Format: | Article |
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MDPI AG
2023-06-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/6/575 |
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| author | Shuangwen Fei Xuan Yang Wentao Xu Jiachao Zhang Jun Li Huamei Chen Xue Lin Sixin Liu Congfa Li |
| author_facet | Shuangwen Fei Xuan Yang Wentao Xu Jiachao Zhang Jun Li Huamei Chen Xue Lin Sixin Liu Congfa Li |
| author_sort | Shuangwen Fei |
| collection | DOAJ |
| description | Nata de coco, known as bacterial cellulose (BC), has been given much attention in the food industry and biomaterial areas due to its specific properties such as low calorie content, high content of fiber, high purity and high biocompatibility. <i>Komagataeibacter</i> spp. are indispensable microorganisms for BC production due to their highly efficient production. Here, proteomics was applied to investigate the metabolism regulation mechanisms of BC yield improvements in <i>K. nataicola</i> Y19 by 48 ± 3% after ethanol supplementation. The results evidenced that differentially expressed proteins involved in the BC biosynthesis system, glycolytic pathway, TCA cycle and oxidative phosphorylation process were up-regulated. The proteins accelerated the BC biosynthesis by providing more energy and via intermediate metabolites. Furthermore, the elongation factor Tu, chaperone DnaK and translocase subunit SecB may be involved in the BC synthesis procedure by regulating electron transfer, hydrolysis of ATP and protein transformation. Moreover, the ethanol-enhanced BC biosynthesis may be associated with the decreased expression of endoglucanase. This research elucidates the proteomics mechanism of higher BC production based on ethanol addition, providing references for nata de coco production efficiency and the synthetic regulation of bacterial cellulose in the future. |
| first_indexed | 2024-03-11T02:28:44Z |
| format | Article |
| id | doaj.art-673ef4f985f34ae98aa3ab08a88f18cf |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T02:28:44Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-673ef4f985f34ae98aa3ab08a88f18cf2023-11-18T10:21:38ZengMDPI AGFermentation2311-56372023-06-019657510.3390/fermentation9060575Insights into Proteomics Reveal Mechanisms of Ethanol-Enhanced Bacterial Cellulose Biosynthesis by <i>Komagataeibacter nataicola</i>Shuangwen Fei0Xuan Yang1Wentao Xu2Jiachao Zhang3Jun Li4Huamei Chen5Xue Lin6Sixin Liu7Congfa Li8School of Food Science and Engineering, Hainan University, Haikou 570228, ChinaSchool of Food Science and Engineering, Hainan University, Haikou 570228, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaSchool of Food Science and Engineering, Hainan University, Haikou 570228, ChinaSchool of Food Science and Engineering, Hainan University, Haikou 570228, ChinaSchool of Food Science and Engineering, Hainan University, Haikou 570228, ChinaSchool of Food Science and Engineering, Hainan University, Haikou 570228, ChinaKey Laboratory of Tropical Agricultural Products Processing Technology of Haikou City, Haikou 570228, ChinaSchool of Food Science and Engineering, Hainan University, Haikou 570228, ChinaNata de coco, known as bacterial cellulose (BC), has been given much attention in the food industry and biomaterial areas due to its specific properties such as low calorie content, high content of fiber, high purity and high biocompatibility. <i>Komagataeibacter</i> spp. are indispensable microorganisms for BC production due to their highly efficient production. Here, proteomics was applied to investigate the metabolism regulation mechanisms of BC yield improvements in <i>K. nataicola</i> Y19 by 48 ± 3% after ethanol supplementation. The results evidenced that differentially expressed proteins involved in the BC biosynthesis system, glycolytic pathway, TCA cycle and oxidative phosphorylation process were up-regulated. The proteins accelerated the BC biosynthesis by providing more energy and via intermediate metabolites. Furthermore, the elongation factor Tu, chaperone DnaK and translocase subunit SecB may be involved in the BC synthesis procedure by regulating electron transfer, hydrolysis of ATP and protein transformation. Moreover, the ethanol-enhanced BC biosynthesis may be associated with the decreased expression of endoglucanase. This research elucidates the proteomics mechanism of higher BC production based on ethanol addition, providing references for nata de coco production efficiency and the synthetic regulation of bacterial cellulose in the future.https://www.mdpi.com/2311-5637/9/6/575nata de cocobacterial cellulosebiosynthesis regulation<i>Komagataeibacter nataicola</i>proteomics |
| spellingShingle | Shuangwen Fei Xuan Yang Wentao Xu Jiachao Zhang Jun Li Huamei Chen Xue Lin Sixin Liu Congfa Li Insights into Proteomics Reveal Mechanisms of Ethanol-Enhanced Bacterial Cellulose Biosynthesis by <i>Komagataeibacter nataicola</i> Fermentation nata de coco bacterial cellulose biosynthesis regulation <i>Komagataeibacter nataicola</i> proteomics |
| title | Insights into Proteomics Reveal Mechanisms of Ethanol-Enhanced Bacterial Cellulose Biosynthesis by <i>Komagataeibacter nataicola</i> |
| title_full | Insights into Proteomics Reveal Mechanisms of Ethanol-Enhanced Bacterial Cellulose Biosynthesis by <i>Komagataeibacter nataicola</i> |
| title_fullStr | Insights into Proteomics Reveal Mechanisms of Ethanol-Enhanced Bacterial Cellulose Biosynthesis by <i>Komagataeibacter nataicola</i> |
| title_full_unstemmed | Insights into Proteomics Reveal Mechanisms of Ethanol-Enhanced Bacterial Cellulose Biosynthesis by <i>Komagataeibacter nataicola</i> |
| title_short | Insights into Proteomics Reveal Mechanisms of Ethanol-Enhanced Bacterial Cellulose Biosynthesis by <i>Komagataeibacter nataicola</i> |
| title_sort | insights into proteomics reveal mechanisms of ethanol enhanced bacterial cellulose biosynthesis by i komagataeibacter nataicola i |
| topic | nata de coco bacterial cellulose biosynthesis regulation <i>Komagataeibacter nataicola</i> proteomics |
| url | https://www.mdpi.com/2311-5637/9/6/575 |
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