Whole-genome sequencing study of KPC-encoding Klebsiella pneumoniae isolated in Greek private laboratories from non-hospitalised patients
Objectives: Greece is endemic for KPC-encoding Klebsiella pneumoniae; however, until now, reports have referred only to hospital isolates. In this study, seven KPC-encoding K. pneumoniae isolated in private laboratories from non-hospitalised patients were characterised. Methods: Whole-genome sequenc...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-03-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716519301936 |
| _version_ | 1819083296777175040 |
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| author | Georgios Meletis Fani Chatzopoulou Aikaterini Fragkouli Magdalini Alexandridou Ilias Mavrovouniotis Anastasia Chatzinikolaou Dimitrios Chatzidimitriou |
| author_facet | Georgios Meletis Fani Chatzopoulou Aikaterini Fragkouli Magdalini Alexandridou Ilias Mavrovouniotis Anastasia Chatzinikolaou Dimitrios Chatzidimitriou |
| author_sort | Georgios Meletis |
| collection | DOAJ |
| description | Objectives: Greece is endemic for KPC-encoding Klebsiella pneumoniae; however, until now, reports have referred only to hospital isolates. In this study, seven KPC-encoding K. pneumoniae isolated in private laboratories from non-hospitalised patients were characterised. Methods: Whole-genome sequencing was performed on an Illumina MiniSeq Sequencing System. Multilocus sequence typing (MLST) was performed using a BLAST-based approach, and antimicrobial resistance genes and plasmid replicons were identified using ResFinder and PlasmidFinder, respectively. The Rapid Annotation using Subsystem Technology (RAST) v.2.0 server was used for genome annotation of virulence, pathogenesis and defence genes. Results: Six isolates belonged to the major MLST sequence type 258 (ST258) and one to ST39. The resistome included genes encoding resistance mechanisms to β-lactams, aminoglycosides, quinolones, sulfonamides, trimethoprim, fosfomycin and phenicols, conferring multidrug-resistant phenotypes. Moreover, various genes involved in virulence, pathogenesis and defence have been identified. Conclusions: It is highly probable that these isolates were acquired during previous hospitalisation in Greek hospitals. The presence of KPC-encoding K. pneumoniae in non-hospitalised patients is alarming, although it is not yet possible to assess its actual impact. |
| first_indexed | 2024-12-21T20:30:19Z |
| format | Article |
| id | doaj.art-6742b1418a864f82bded0f6827cb8a63 |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-12-21T20:30:19Z |
| publishDate | 2020-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-6742b1418a864f82bded0f6827cb8a632022-12-21T18:51:14ZengElsevierJournal of Global Antimicrobial Resistance2213-71652020-03-01207881Whole-genome sequencing study of KPC-encoding Klebsiella pneumoniae isolated in Greek private laboratories from non-hospitalised patientsGeorgios Meletis0Fani Chatzopoulou1Aikaterini Fragkouli2Magdalini Alexandridou3Ilias Mavrovouniotis4Anastasia Chatzinikolaou5Dimitrios Chatzidimitriou6Labnet Laboratories, Agiou Dimitriou str. 161, 54638 Thessaloniki, Greece; Corresponding author.Labnet Laboratories, Agiou Dimitriou str. 161, 54638 Thessaloniki, Greece; Laboratory of Microbiology, Medical School, Aristotle University of Thessaloniki, Thessaloniki, GreeceLabnet Laboratories, Agiou Dimitriou str. 161, 54638 Thessaloniki, GreecePrivate Biopathology Laboratory, GreecePrivate Biopathology Laboratory, GreecePrivate Biopathology Laboratory, GreeceLabnet Laboratories, Agiou Dimitriou str. 161, 54638 Thessaloniki, Greece; Laboratory of Microbiology, Medical School, Aristotle University of Thessaloniki, Thessaloniki, GreeceObjectives: Greece is endemic for KPC-encoding Klebsiella pneumoniae; however, until now, reports have referred only to hospital isolates. In this study, seven KPC-encoding K. pneumoniae isolated in private laboratories from non-hospitalised patients were characterised. Methods: Whole-genome sequencing was performed on an Illumina MiniSeq Sequencing System. Multilocus sequence typing (MLST) was performed using a BLAST-based approach, and antimicrobial resistance genes and plasmid replicons were identified using ResFinder and PlasmidFinder, respectively. The Rapid Annotation using Subsystem Technology (RAST) v.2.0 server was used for genome annotation of virulence, pathogenesis and defence genes. Results: Six isolates belonged to the major MLST sequence type 258 (ST258) and one to ST39. The resistome included genes encoding resistance mechanisms to β-lactams, aminoglycosides, quinolones, sulfonamides, trimethoprim, fosfomycin and phenicols, conferring multidrug-resistant phenotypes. Moreover, various genes involved in virulence, pathogenesis and defence have been identified. Conclusions: It is highly probable that these isolates were acquired during previous hospitalisation in Greek hospitals. The presence of KPC-encoding K. pneumoniae in non-hospitalised patients is alarming, although it is not yet possible to assess its actual impact.http://www.sciencedirect.com/science/article/pii/S2213716519301936Klebsiella pneumoniaeKPCST258ST39Whole-genome sequencingGreece |
| spellingShingle | Georgios Meletis Fani Chatzopoulou Aikaterini Fragkouli Magdalini Alexandridou Ilias Mavrovouniotis Anastasia Chatzinikolaou Dimitrios Chatzidimitriou Whole-genome sequencing study of KPC-encoding Klebsiella pneumoniae isolated in Greek private laboratories from non-hospitalised patients Journal of Global Antimicrobial Resistance Klebsiella pneumoniae KPC ST258 ST39 Whole-genome sequencing Greece |
| title | Whole-genome sequencing study of KPC-encoding Klebsiella pneumoniae isolated in Greek private laboratories from non-hospitalised patients |
| title_full | Whole-genome sequencing study of KPC-encoding Klebsiella pneumoniae isolated in Greek private laboratories from non-hospitalised patients |
| title_fullStr | Whole-genome sequencing study of KPC-encoding Klebsiella pneumoniae isolated in Greek private laboratories from non-hospitalised patients |
| title_full_unstemmed | Whole-genome sequencing study of KPC-encoding Klebsiella pneumoniae isolated in Greek private laboratories from non-hospitalised patients |
| title_short | Whole-genome sequencing study of KPC-encoding Klebsiella pneumoniae isolated in Greek private laboratories from non-hospitalised patients |
| title_sort | whole genome sequencing study of kpc encoding klebsiella pneumoniae isolated in greek private laboratories from non hospitalised patients |
| topic | Klebsiella pneumoniae KPC ST258 ST39 Whole-genome sequencing Greece |
| url | http://www.sciencedirect.com/science/article/pii/S2213716519301936 |
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