Exploring genetic alterations in circulating tumor DNA from cerebrospinal fluid of pediatric medulloblastoma
Abstract Medulloblastoma (MB) is the most common type of brain malignancy in children. Molecular profiling has become an important component to select patients for therapeutic approaches, allowing for personalized therapy. In this study, we successfully identified detectable levels of tumor-derived...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-85178-6 |
| _version_ | 1818735281089544192 |
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| author | Yanling Sun Miao Li Siqi Ren Yan Liu Jin Zhang Shuting Li Wenchao Gao Xiaojun Gong Jingjing Liu Yuan Wang Shuxu Du Liming Sun Wanshui Wu Yongji Tian |
| author_facet | Yanling Sun Miao Li Siqi Ren Yan Liu Jin Zhang Shuting Li Wenchao Gao Xiaojun Gong Jingjing Liu Yuan Wang Shuxu Du Liming Sun Wanshui Wu Yongji Tian |
| author_sort | Yanling Sun |
| collection | DOAJ |
| description | Abstract Medulloblastoma (MB) is the most common type of brain malignancy in children. Molecular profiling has become an important component to select patients for therapeutic approaches, allowing for personalized therapy. In this study, we successfully identified detectable levels of tumor-derived cell-free DNA (cfDNA) in cerebrospinal fluid (CSF) samples of patients with MB. Furthermore, cfDNA from CSF can interrogate for tumor-associated molecular clues. MB-associated alterations from CSF, tumor, and post-chemotherapy plasma were compared by deep sequencing on next-generation sequencing platform. Shared alterations exist between CSF and matched tumor tissues. More alternations were detected in circulating tumor DNA from CSF than those in genomic DNA from primary tumor. It was feasible to detect MB-associated mutations in plasma of patients treated with chemotherapy. Collectively, CSF supernatant can be used to monitor genomic alterations, as a superior technique as long as tumor-derived cfDNA can be isolated from CSF successfully. |
| first_indexed | 2024-12-18T00:18:45Z |
| format | Article |
| id | doaj.art-67450cab29a44b85b5b48b10b84d70e3 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T00:18:45Z |
| publishDate | 2021-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-67450cab29a44b85b5b48b10b84d70e32022-12-21T21:27:24ZengNature PortfolioScientific Reports2045-23222021-03-011111810.1038/s41598-021-85178-6Exploring genetic alterations in circulating tumor DNA from cerebrospinal fluid of pediatric medulloblastomaYanling Sun0Miao Li1Siqi Ren2Yan Liu3Jin Zhang4Shuting Li5Wenchao Gao6Xiaojun Gong7Jingjing Liu8Yuan Wang9Shuxu Du10Liming Sun11Wanshui Wu12Yongji Tian13Department of Pediatrics, Beijing Shijitan Hospital, Capital Medical UniversityDepartment of Pediatrics, Beijing Shijitan Hospital, Capital Medical UniversityDepartment of Pediatrics, Beijing Shijitan Hospital, Capital Medical UniversityDepartment of Pediatrics, Beijing Shijitan Hospital, Capital Medical UniversityDepartment of Pediatrics, Beijing Shijitan Hospital, Capital Medical UniversityDepartment of Pediatrics, Beijing Shijitan Hospital, Capital Medical UniversityDepartment of Pediatrics, Beijing Shijitan Hospital, Capital Medical UniversityDepartment of Pediatrics, Beijing Shijitan Hospital, Capital Medical UniversityDepartment of Pediatrics, Beijing Shijitan Hospital, Capital Medical UniversityDepartment of Pediatrics, Beijing Shijitan Hospital, Capital Medical UniversityDepartment of Pediatrics, Beijing Shijitan Hospital, Capital Medical UniversityDepartment of Pediatrics, Beijing Shijitan Hospital, Capital Medical UniversityDepartment of Pediatrics, Beijing Shijitan Hospital, Capital Medical UniversityDepartment of Pediatric Neurosurgery, Beijing Tiantan Hospital, Capital Medical UniversityAbstract Medulloblastoma (MB) is the most common type of brain malignancy in children. Molecular profiling has become an important component to select patients for therapeutic approaches, allowing for personalized therapy. In this study, we successfully identified detectable levels of tumor-derived cell-free DNA (cfDNA) in cerebrospinal fluid (CSF) samples of patients with MB. Furthermore, cfDNA from CSF can interrogate for tumor-associated molecular clues. MB-associated alterations from CSF, tumor, and post-chemotherapy plasma were compared by deep sequencing on next-generation sequencing platform. Shared alterations exist between CSF and matched tumor tissues. More alternations were detected in circulating tumor DNA from CSF than those in genomic DNA from primary tumor. It was feasible to detect MB-associated mutations in plasma of patients treated with chemotherapy. Collectively, CSF supernatant can be used to monitor genomic alterations, as a superior technique as long as tumor-derived cfDNA can be isolated from CSF successfully.https://doi.org/10.1038/s41598-021-85178-6 |
| spellingShingle | Yanling Sun Miao Li Siqi Ren Yan Liu Jin Zhang Shuting Li Wenchao Gao Xiaojun Gong Jingjing Liu Yuan Wang Shuxu Du Liming Sun Wanshui Wu Yongji Tian Exploring genetic alterations in circulating tumor DNA from cerebrospinal fluid of pediatric medulloblastoma Scientific Reports |
| title | Exploring genetic alterations in circulating tumor DNA from cerebrospinal fluid of pediatric medulloblastoma |
| title_full | Exploring genetic alterations in circulating tumor DNA from cerebrospinal fluid of pediatric medulloblastoma |
| title_fullStr | Exploring genetic alterations in circulating tumor DNA from cerebrospinal fluid of pediatric medulloblastoma |
| title_full_unstemmed | Exploring genetic alterations in circulating tumor DNA from cerebrospinal fluid of pediatric medulloblastoma |
| title_short | Exploring genetic alterations in circulating tumor DNA from cerebrospinal fluid of pediatric medulloblastoma |
| title_sort | exploring genetic alterations in circulating tumor dna from cerebrospinal fluid of pediatric medulloblastoma |
| url | https://doi.org/10.1038/s41598-021-85178-6 |
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