Using dried blood spot for the detection of HBsAg and anti-HCV antibodies in Cameroon
Abstract Objective Dried blood spots (DBS) offer multiple benefits for collecting, storing and shipping whole blood samples. Our objective was to compare, for the first time in Africa, the performance of DBS with respect to plasma in the detection of Hepatitis B surface antigen (HBsAg) and antibodie...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-11-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13104-018-3931-3 |
| _version_ | 1818130887785578496 |
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| author | Sebastien Kenmoe Paul Alain Ngoupo Tagnouokam Cyprien Kengne Nde Ghislaine Flore Mella-Tamko Richard Njouom |
| author_facet | Sebastien Kenmoe Paul Alain Ngoupo Tagnouokam Cyprien Kengne Nde Ghislaine Flore Mella-Tamko Richard Njouom |
| author_sort | Sebastien Kenmoe |
| collection | DOAJ |
| description | Abstract Objective Dried blood spots (DBS) offer multiple benefits for collecting, storing and shipping whole blood samples. Our objective was to compare, for the first time in Africa, the performance of DBS with respect to plasma in the detection of Hepatitis B surface antigen (HBsAg) and antibodies to Hepatitis C Virus (anti-HCV) using Architect, Abbott Diagnostics. Results DBS had a sensitivity of 99%, a specificity of 100%, a positive predictive value of 99%, a negative predictive value of 100% and a kappa index of 0.99 for the detection of HBsAg. For anti-HCV detection, the sensitivity, specificity, positive predictive value, negative predictive value and kappa index were 99%, 98%, 98%, 99%, and 0.97, respectively. This study confirms that DBS may be a reliable alternative specimen type for HBV and HCV diagnosis. |
| first_indexed | 2024-12-11T08:12:11Z |
| format | Article |
| id | doaj.art-6746eca179a54747ad48ee4c3d4a67bc |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-11T08:12:11Z |
| publishDate | 2018-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-6746eca179a54747ad48ee4c3d4a67bc2022-12-22T01:14:51ZengBMCBMC Research Notes1756-05002018-11-011111410.1186/s13104-018-3931-3Using dried blood spot for the detection of HBsAg and anti-HCV antibodies in CameroonSebastien Kenmoe0Paul Alain Ngoupo Tagnouokam1Cyprien Kengne Nde2Ghislaine Flore Mella-Tamko3Richard Njouom4Virology Department, Centre Pasteur du CamerounVirology Department, Centre Pasteur du CamerounNational AIDS Control CommitteeVirology Department, Centre Pasteur du CamerounVirology Department, Centre Pasteur du CamerounAbstract Objective Dried blood spots (DBS) offer multiple benefits for collecting, storing and shipping whole blood samples. Our objective was to compare, for the first time in Africa, the performance of DBS with respect to plasma in the detection of Hepatitis B surface antigen (HBsAg) and antibodies to Hepatitis C Virus (anti-HCV) using Architect, Abbott Diagnostics. Results DBS had a sensitivity of 99%, a specificity of 100%, a positive predictive value of 99%, a negative predictive value of 100% and a kappa index of 0.99 for the detection of HBsAg. For anti-HCV detection, the sensitivity, specificity, positive predictive value, negative predictive value and kappa index were 99%, 98%, 98%, 99%, and 0.97, respectively. This study confirms that DBS may be a reliable alternative specimen type for HBV and HCV diagnosis.http://link.springer.com/article/10.1186/s13104-018-3931-3Dried blood spotDiagnosisHepatitis BHepatitis CCameroon |
| spellingShingle | Sebastien Kenmoe Paul Alain Ngoupo Tagnouokam Cyprien Kengne Nde Ghislaine Flore Mella-Tamko Richard Njouom Using dried blood spot for the detection of HBsAg and anti-HCV antibodies in Cameroon BMC Research Notes Dried blood spot Diagnosis Hepatitis B Hepatitis C Cameroon |
| title | Using dried blood spot for the detection of HBsAg and anti-HCV antibodies in Cameroon |
| title_full | Using dried blood spot for the detection of HBsAg and anti-HCV antibodies in Cameroon |
| title_fullStr | Using dried blood spot for the detection of HBsAg and anti-HCV antibodies in Cameroon |
| title_full_unstemmed | Using dried blood spot for the detection of HBsAg and anti-HCV antibodies in Cameroon |
| title_short | Using dried blood spot for the detection of HBsAg and anti-HCV antibodies in Cameroon |
| title_sort | using dried blood spot for the detection of hbsag and anti hcv antibodies in cameroon |
| topic | Dried blood spot Diagnosis Hepatitis B Hepatitis C Cameroon |
| url | http://link.springer.com/article/10.1186/s13104-018-3931-3 |
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