Visualizing the dental biofilm matrix by means of fluorescence lectin-binding analysis
The extracellular matrix is a poorly studied, yet important component of dental biofilms. Fluorescence lectin-binding analysis (FLBA) is a powerful tool to characterize glycoconjugates in the biofilm matrix. This study aimed to systematically investigate the ability of 75 fluorescently labeled lecti...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2017-01-01
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| Series: | Journal of Oral Microbiology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/20002297.2017.1345581 |
| _version_ | 1818205236921106432 |
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| author | Pune N. Tawakoli Thomas R. Neu Mette M. Busck Ute Kuhlicke Andreas Schramm Thomas Attin Daniel B. Wiedemeier Sebastian Schlafer |
| author_facet | Pune N. Tawakoli Thomas R. Neu Mette M. Busck Ute Kuhlicke Andreas Schramm Thomas Attin Daniel B. Wiedemeier Sebastian Schlafer |
| author_sort | Pune N. Tawakoli |
| collection | DOAJ |
| description | The extracellular matrix is a poorly studied, yet important component of dental biofilms. Fluorescence lectin-binding analysis (FLBA) is a powerful tool to characterize glycoconjugates in the biofilm matrix. This study aimed to systematically investigate the ability of 75 fluorescently labeled lectins to visualize and quantify extracellular glycoconjugates in dental biofilms. Lectin binding was screened on pooled supragingival biofilm samples collected from 76 subjects using confocal microscopy. FLBA was then performed with 10 selected lectins on biofilms grown in situ for 48 h in the absence of sucrose. For five lectins that proved particularly suitable, stained biovolumes were quantified and correlated to the bacterial composition of the biofilms. Additionally, combinations of up to three differently labeled lectins were tested. Of the 10 lectins, five bound particularly well in 48-h-biofilms: Aleuria aurantia (AAL), Calystega sepiem (Calsepa), Lycopersicon esculentum (LEA), Morniga-G (MNA-G) and Helix pomatia (HPA). No significant correlation between the binding of specific lectins and bacterial composition was found. Fluorescently labeled lectins enable the visualization of glycoconjugates in the dental biofilm matrix. The characterization and quantification of glycoconjugates in dental biofilms require a combination of several lectins. For 48-h-biofilms grown in absence of sucrose, AAL, Calsepa, HPA, LEA, and MNA-G are recommendable. |
| first_indexed | 2024-12-12T03:53:56Z |
| format | Article |
| id | doaj.art-67473e97c9804d28872dc8f713d12020 |
| institution | Directory Open Access Journal |
| issn | 2000-2297 |
| language | English |
| last_indexed | 2024-12-12T03:53:56Z |
| publishDate | 2017-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj.art-67473e97c9804d28872dc8f713d120202022-12-22T00:39:18ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972017-01-019110.1080/20002297.2017.13455811345581Visualizing the dental biofilm matrix by means of fluorescence lectin-binding analysisPune N. Tawakoli0Thomas R. Neu1Mette M. Busck2Ute Kuhlicke3Andreas Schramm4Thomas Attin5Daniel B. Wiedemeier6Sebastian Schlafer7University of ZurichHelmholtz Centre for Environmental Research – UFZAarhus UniversityHelmholtz Centre for Environmental Research – UFZAarhus UniversityUniversity of ZurichUniversity of ZurichAarhus UniversityThe extracellular matrix is a poorly studied, yet important component of dental biofilms. Fluorescence lectin-binding analysis (FLBA) is a powerful tool to characterize glycoconjugates in the biofilm matrix. This study aimed to systematically investigate the ability of 75 fluorescently labeled lectins to visualize and quantify extracellular glycoconjugates in dental biofilms. Lectin binding was screened on pooled supragingival biofilm samples collected from 76 subjects using confocal microscopy. FLBA was then performed with 10 selected lectins on biofilms grown in situ for 48 h in the absence of sucrose. For five lectins that proved particularly suitable, stained biovolumes were quantified and correlated to the bacterial composition of the biofilms. Additionally, combinations of up to three differently labeled lectins were tested. Of the 10 lectins, five bound particularly well in 48-h-biofilms: Aleuria aurantia (AAL), Calystega sepiem (Calsepa), Lycopersicon esculentum (LEA), Morniga-G (MNA-G) and Helix pomatia (HPA). No significant correlation between the binding of specific lectins and bacterial composition was found. Fluorescently labeled lectins enable the visualization of glycoconjugates in the dental biofilm matrix. The characterization and quantification of glycoconjugates in dental biofilms require a combination of several lectins. For 48-h-biofilms grown in absence of sucrose, AAL, Calsepa, HPA, LEA, and MNA-G are recommendable.http://dx.doi.org/10.1080/20002297.2017.1345581Confocal laser scanning microscopydental biofilmsextracellular polymeric substancesglycoconjugateslectins |
| spellingShingle | Pune N. Tawakoli Thomas R. Neu Mette M. Busck Ute Kuhlicke Andreas Schramm Thomas Attin Daniel B. Wiedemeier Sebastian Schlafer Visualizing the dental biofilm matrix by means of fluorescence lectin-binding analysis Journal of Oral Microbiology Confocal laser scanning microscopy dental biofilms extracellular polymeric substances glycoconjugates lectins |
| title | Visualizing the dental biofilm matrix by means of fluorescence lectin-binding analysis |
| title_full | Visualizing the dental biofilm matrix by means of fluorescence lectin-binding analysis |
| title_fullStr | Visualizing the dental biofilm matrix by means of fluorescence lectin-binding analysis |
| title_full_unstemmed | Visualizing the dental biofilm matrix by means of fluorescence lectin-binding analysis |
| title_short | Visualizing the dental biofilm matrix by means of fluorescence lectin-binding analysis |
| title_sort | visualizing the dental biofilm matrix by means of fluorescence lectin binding analysis |
| topic | Confocal laser scanning microscopy dental biofilms extracellular polymeric substances glycoconjugates lectins |
| url | http://dx.doi.org/10.1080/20002297.2017.1345581 |
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