Efficient Anchoring of <i>Erianthus</i> <i>arundinaceus</i> Chromatin Introgressed into Sugarcane by Specific Molecular Markers
<i>Erianthus</i> <i>arundinaceus</i> is a valuable gene reservoir for sugarcane improvement. However, insufficient molecular markers for high-accuracy identification and tracking of the introgression status of <i>E.</i> <i>arundinaceus</i> chromatin im...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/16/9435 |
| Summary: | <i>Erianthus</i> <i>arundinaceus</i> is a valuable gene reservoir for sugarcane improvement. However, insufficient molecular markers for high-accuracy identification and tracking of the introgression status of <i>E.</i> <i>arundinaceus</i> chromatin impede sugarcane breeding. Fortunately, suppression subtractive hybridization (SSH) technology provides an excellent opportunity for the development of high-throughput <i>E.</i> <i>arundinaceus</i>-specific molecular markers at a reasonable cost. In this study, we constructed a SSH library of <i>E.</i> <i>arundinaceus</i>. In total, 288 clones of <i>E.</i> <i>arundinaceus</i>-specific repetitive sequences were screened out and their distribution patterns on chromosomes were characterized by fluorescence in situ hybridization (FISH). A subtelomeric repetitive sequence Ea086 and a diffusive repetitive sequence Ea009, plus 45S rDNA-bearing <i>E.</i> <i>arundinaceus</i> chromosome repetitive sequence EaITS were developed as <i>E.</i> <i>arundinaceus</i>-specific molecular markers, namely, Ea086-128, Ea009-257, and EaITS-278, covering all the <i>E.</i> <i>arundinaceus</i> chromosomes for high-accuracy identification of putative progeny. Both Ea086-128 and Ea009-257 were successfully applied to identify the authenticity of F<sub>1</sub>, BC<sub>1</sub>, BC<sub>2</sub>, BC<sub>3</sub>, and BC<sub>4</sub> progeny between sugarcane and <i>E.</i> <i>arundinaceus</i>. In addition, EaITS-278 was a 45S rDNA-bearing <i>E.</i> <i>arundinaceus</i> chromosome-specific molecular marker for rapid tracking of the inherited status of this chromosome in a sugarcane background. Three BC<sub>3</sub> progeny had apparently lost the 45S rDNA-bearing <i>E.</i> <i>arundinaceus</i> chromosome. We reported herein a highly effective and reliable SSH-based technology for discovery of high-throughput <i>E.</i> <i>arundinaceus</i>-specific sequences bearing high potential as molecular markers. Given its reliability and savings in time and efforts, the method is also suitable for development of species-specific molecular markers for other important wild relatives to accelerate introgression of wild relatives into sugarcane. |
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| ISSN: | 1661-6596 1422-0067 |