Liver cirrhosis contributes to the disorder of gut microbiota in patients with hepatocellular carcinoma
Abstract Background Gut microbiota (GM) of patients with liver cancer is disordered, and syet no study reported the GM distribution of liver cirrhosis‐induced HCC (LC‐HCC) and nonliver cirrhosis‐induced HCC (NLC‐HCC). In this study, we aimed to characterize gut dysbiosis of LC‐HCC and NLC‐HCC to elu...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-06-01
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| Series: | Cancer Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/cam4.3045 |
| _version_ | 1798039648541343744 |
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| author | Ruipeng Zheng Guoqiang Wang Zhiqiang Pang Nan Ran Yinuo Gu Xuewa Guan Yuze Yuan Xu Zuo He Pan Jingtong Zheng Fang Wang |
| author_facet | Ruipeng Zheng Guoqiang Wang Zhiqiang Pang Nan Ran Yinuo Gu Xuewa Guan Yuze Yuan Xu Zuo He Pan Jingtong Zheng Fang Wang |
| author_sort | Ruipeng Zheng |
| collection | DOAJ |
| description | Abstract Background Gut microbiota (GM) of patients with liver cancer is disordered, and syet no study reported the GM distribution of liver cirrhosis‐induced HCC (LC‐HCC) and nonliver cirrhosis‐induced HCC (NLC‐HCC). In this study, we aimed to characterize gut dysbiosis of LC‐HCC and NLC‐HCC to elucidate the role of GM in the pathogenesis of HCC. Methods A consecutive series of fecal samples of patients with hepatitis (24 patients), liver cirrhosis (24 patients), HCC (75 patients: 35 infected by HBV, 25 infected by HCV, and 15 with alcoholic liver disease), and healthy controls (20 patients) were obtained and sequenced on the Illumina Hiseq platform. The HCC group contains 52 LC‐HCC and 23 NLC‐HCC. Bioinformatic analysis of the intestinal microbiota was performed with QIIME and MicrobiomeAnalyst. Results Alpha‐diversity analysis showed that fecal microbial diversity was significantly decreased in the LC group, and there were significant differences in 3 phyla and 27 genera in the LC group vs the other groups (the healthy, hepatitis, and HCC groups). Beta‐diversity analysis showed that there were large differences between LC and the others. Gut microbial diversity was significantly increased from LC to HCC. Characterizing the fecal microbiota of LC‐HCC and NLC‐HCC, we found that microbial diversity was increased from LC to LC‐HCC rather than NLC‐HCC. Thirteen genera were discovered to be associated with the tumor size of HCC. Three biomarkers (Enterococcus, Limnobacter, and Phyllobacterium) could be used for precision diagnosis. We also found that HBV infection, HCV infection, or ALD (alcoholic liver disease) was not associated with intestinal microbial dysbiosis in HCC. Conclusion Our results suggest that GM disorders are more common in patients with LC‐HCC. The butyrate‐producing genera were decreased, while genera producing‐lipopolysaccharide (LPS) were increased in LC‐HCC patients. Further studies of GM disorders may achieve early diagnosis and new therapeutic approaches for HCC patients. |
| first_indexed | 2024-04-11T21:56:47Z |
| format | Article |
| id | doaj.art-674a84579b3e417cae63227a75a9b043 |
| institution | Directory Open Access Journal |
| issn | 2045-7634 |
| language | English |
| last_indexed | 2024-04-11T21:56:47Z |
| publishDate | 2020-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj.art-674a84579b3e417cae63227a75a9b0432022-12-22T04:01:04ZengWileyCancer Medicine2045-76342020-06-019124232425010.1002/cam4.3045Liver cirrhosis contributes to the disorder of gut microbiota in patients with hepatocellular carcinomaRuipeng Zheng0Guoqiang Wang1Zhiqiang Pang2Nan Ran3Yinuo Gu4Xuewa Guan5Yuze Yuan6Xu Zuo7He Pan8Jingtong Zheng9Fang Wang10Department of Pathogen Biology College of Basic Medical Sciences Jilin University Changchun ChinaDepartment of Pathogen Biology College of Basic Medical Sciences Jilin University Changchun ChinaDepartment of Pathogen Biology College of Basic Medical Sciences Jilin University Changchun ChinaDepartment of Pathogen Biology College of Basic Medical Sciences Jilin University Changchun ChinaDepartment of Pathogen Biology College of Basic Medical Sciences Jilin University Changchun ChinaDepartment of Pathogen Biology College of Basic Medical Sciences Jilin University Changchun ChinaDepartment of Pathogen Biology College of Basic Medical Sciences Jilin University Changchun ChinaDepartment of Pathogen Biology College of Basic Medical Sciences Jilin University Changchun ChinaDepartment of Pathogen Biology College of Basic Medical Sciences Jilin University Changchun ChinaDepartment of Pathogen Biology College of Basic Medical Sciences Jilin University Changchun ChinaDepartment of Pathogen Biology College of Basic Medical Sciences Jilin University Changchun ChinaAbstract Background Gut microbiota (GM) of patients with liver cancer is disordered, and syet no study reported the GM distribution of liver cirrhosis‐induced HCC (LC‐HCC) and nonliver cirrhosis‐induced HCC (NLC‐HCC). In this study, we aimed to characterize gut dysbiosis of LC‐HCC and NLC‐HCC to elucidate the role of GM in the pathogenesis of HCC. Methods A consecutive series of fecal samples of patients with hepatitis (24 patients), liver cirrhosis (24 patients), HCC (75 patients: 35 infected by HBV, 25 infected by HCV, and 15 with alcoholic liver disease), and healthy controls (20 patients) were obtained and sequenced on the Illumina Hiseq platform. The HCC group contains 52 LC‐HCC and 23 NLC‐HCC. Bioinformatic analysis of the intestinal microbiota was performed with QIIME and MicrobiomeAnalyst. Results Alpha‐diversity analysis showed that fecal microbial diversity was significantly decreased in the LC group, and there were significant differences in 3 phyla and 27 genera in the LC group vs the other groups (the healthy, hepatitis, and HCC groups). Beta‐diversity analysis showed that there were large differences between LC and the others. Gut microbial diversity was significantly increased from LC to HCC. Characterizing the fecal microbiota of LC‐HCC and NLC‐HCC, we found that microbial diversity was increased from LC to LC‐HCC rather than NLC‐HCC. Thirteen genera were discovered to be associated with the tumor size of HCC. Three biomarkers (Enterococcus, Limnobacter, and Phyllobacterium) could be used for precision diagnosis. We also found that HBV infection, HCV infection, or ALD (alcoholic liver disease) was not associated with intestinal microbial dysbiosis in HCC. Conclusion Our results suggest that GM disorders are more common in patients with LC‐HCC. The butyrate‐producing genera were decreased, while genera producing‐lipopolysaccharide (LPS) were increased in LC‐HCC patients. Further studies of GM disorders may achieve early diagnosis and new therapeutic approaches for HCC patients.https://doi.org/10.1002/cam4.3045gut microbiotahepatitishepatocellular carcinomaliver cirrhosis |
| spellingShingle | Ruipeng Zheng Guoqiang Wang Zhiqiang Pang Nan Ran Yinuo Gu Xuewa Guan Yuze Yuan Xu Zuo He Pan Jingtong Zheng Fang Wang Liver cirrhosis contributes to the disorder of gut microbiota in patients with hepatocellular carcinoma Cancer Medicine gut microbiota hepatitis hepatocellular carcinoma liver cirrhosis |
| title | Liver cirrhosis contributes to the disorder of gut microbiota in patients with hepatocellular carcinoma |
| title_full | Liver cirrhosis contributes to the disorder of gut microbiota in patients with hepatocellular carcinoma |
| title_fullStr | Liver cirrhosis contributes to the disorder of gut microbiota in patients with hepatocellular carcinoma |
| title_full_unstemmed | Liver cirrhosis contributes to the disorder of gut microbiota in patients with hepatocellular carcinoma |
| title_short | Liver cirrhosis contributes to the disorder of gut microbiota in patients with hepatocellular carcinoma |
| title_sort | liver cirrhosis contributes to the disorder of gut microbiota in patients with hepatocellular carcinoma |
| topic | gut microbiota hepatitis hepatocellular carcinoma liver cirrhosis |
| url | https://doi.org/10.1002/cam4.3045 |
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