Application of Western Blotting for the Post-Treatment Monitoring of Human Cystic Echinococcosis.
Cystic echinococcosis (CE) is one of the most important parasitic zoonosis worldwide. Due to the high recurrence rate of the disease after surgery, follow up of the patient is necessary. The aim of current research was to assess the performance of Western blotting (WB), using sheep hydatid fluid, fo...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2013-08-01
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| Series: | Iranian Journal of Public Health |
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| Online Access: | https://ijph.tums.ac.ir/index.php/ijph/article/view/4438 |
| _version_ | 1819058814908891136 |
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| author | Rajesh Kumar Tenguria Mohd Irfan Naik Bashir Fomda |
| author_facet | Rajesh Kumar Tenguria Mohd Irfan Naik Bashir Fomda |
| author_sort | Rajesh Kumar Tenguria |
| collection | DOAJ |
| description | Cystic echinococcosis (CE) is one of the most important parasitic zoonosis worldwide. Due to the high recurrence rate of the disease after surgery, follow up of the patient is necessary. The aim of current research was to assess the performance of Western blotting (WB), using sheep hydatid fluid, for serological diagnosis and post-treatment monitoring of human CE.Serum samples obtained from 50 clinically/radiologically proven cases of CE along with serum samples from non-CE patients and healthy persons were tested by WB, using sheep hydatid fluid as antigen.The WB test enabled the detection of antibodies in the pre-operative samples for proteins of 18-239 kDa in molecular weight. From 50 sera of CE patients, 31 cases (72.09%) detected 52 kDa subunit, 27 cases (62.79%) detected 24 kDa band, 26 cases (60.46%) recognised 39 kDa band and 21 cases (48.83%) identified 46 kDa component of sheep hydatid antigen. Sera from patients with other parasitic infections and malignancy showed cross-reactivity with the cluster of 54-59 kDa bands. The healthy control sera were not reactive to any antigenic fraction. The antigenic bands with molecular weight of 52, 24, 39 and 46 kDa were specific for CE, and may serve as useful diagnostic markers. The antibodies specific to proteins 24 and 39 kDa significantly decreased in the patients cured after surgery, while in patients with recurrent parasitism the bands present before surgery persisted.The WB with sheep hydatid antigen might be useful in the diagnosis and post-surgical monitoring of CE patients. |
| first_indexed | 2024-12-21T14:01:11Z |
| format | Article |
| id | doaj.art-674b1dbcfac44ea88298045bd024ac35 |
| institution | Directory Open Access Journal |
| issn | 2251-6085 2251-6093 |
| language | English |
| last_indexed | 2024-12-21T14:01:11Z |
| publishDate | 2013-08-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-674b1dbcfac44ea88298045bd024ac352022-12-21T19:01:21ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852251-60932013-08-01428Application of Western Blotting for the Post-Treatment Monitoring of Human Cystic Echinococcosis.Rajesh Kumar Tenguria0Mohd Irfan Naik1Bashir Fomda2Division of Microbiology, Department of Botany, Govt. Motilal Vigyan Mahavidyalya, Bhopal, India.Division of Microbiology, Department of Botany, Govt. Motilal Vigyan Mahavidyalya, Bhopal, India.Dept. of Microbiology, Sher-i-Kashmir Institute of Medical Sciences, Srinagar Kashmir, India.Cystic echinococcosis (CE) is one of the most important parasitic zoonosis worldwide. Due to the high recurrence rate of the disease after surgery, follow up of the patient is necessary. The aim of current research was to assess the performance of Western blotting (WB), using sheep hydatid fluid, for serological diagnosis and post-treatment monitoring of human CE.Serum samples obtained from 50 clinically/radiologically proven cases of CE along with serum samples from non-CE patients and healthy persons were tested by WB, using sheep hydatid fluid as antigen.The WB test enabled the detection of antibodies in the pre-operative samples for proteins of 18-239 kDa in molecular weight. From 50 sera of CE patients, 31 cases (72.09%) detected 52 kDa subunit, 27 cases (62.79%) detected 24 kDa band, 26 cases (60.46%) recognised 39 kDa band and 21 cases (48.83%) identified 46 kDa component of sheep hydatid antigen. Sera from patients with other parasitic infections and malignancy showed cross-reactivity with the cluster of 54-59 kDa bands. The healthy control sera were not reactive to any antigenic fraction. The antigenic bands with molecular weight of 52, 24, 39 and 46 kDa were specific for CE, and may serve as useful diagnostic markers. The antibodies specific to proteins 24 and 39 kDa significantly decreased in the patients cured after surgery, while in patients with recurrent parasitism the bands present before surgery persisted.The WB with sheep hydatid antigen might be useful in the diagnosis and post-surgical monitoring of CE patients.https://ijph.tums.ac.ir/index.php/ijph/article/view/4438AntigensEchinococcus granulosusHydatidosisTreatmentWestern blot |
| spellingShingle | Rajesh Kumar Tenguria Mohd Irfan Naik Bashir Fomda Application of Western Blotting for the Post-Treatment Monitoring of Human Cystic Echinococcosis. Iranian Journal of Public Health Antigens Echinococcus granulosus Hydatidosis Treatment Western blot |
| title | Application of Western Blotting for the Post-Treatment Monitoring of Human Cystic Echinococcosis. |
| title_full | Application of Western Blotting for the Post-Treatment Monitoring of Human Cystic Echinococcosis. |
| title_fullStr | Application of Western Blotting for the Post-Treatment Monitoring of Human Cystic Echinococcosis. |
| title_full_unstemmed | Application of Western Blotting for the Post-Treatment Monitoring of Human Cystic Echinococcosis. |
| title_short | Application of Western Blotting for the Post-Treatment Monitoring of Human Cystic Echinococcosis. |
| title_sort | application of western blotting for the post treatment monitoring of human cystic echinococcosis |
| topic | Antigens Echinococcus granulosus Hydatidosis Treatment Western blot |
| url | https://ijph.tums.ac.ir/index.php/ijph/article/view/4438 |
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