Phenolic Acids-Rich Fractions from <i>Agaricus bitorguis</i> (Quél.) Sacc. Chaidam ZJU-CDMA-12 Mycelia Modulate Hypoxic Stress on Hypoxia-Damaged PC12 Cells
Hypoxia is a common pathological process in various clinical diseases. However, there is still a lack of effective anti-hypoxia active substances. <i>Agaricus bitorguis</i> (Quél.) Sacc Chaidam (ABSC) is a rare wild edible macrofungus that grows underground at high altitudes. Herein, int...
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MDPI AG
2020-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/20/4845 |
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| author | Hongyun Lu Zhihua Jiao Yingchun Jiao Wei Wang Qihe Chen |
| author_facet | Hongyun Lu Zhihua Jiao Yingchun Jiao Wei Wang Qihe Chen |
| author_sort | Hongyun Lu |
| collection | DOAJ |
| description | Hypoxia is a common pathological process in various clinical diseases. However, there is still a lack of effective anti-hypoxia active substances. <i>Agaricus bitorguis</i> (Quél.) Sacc Chaidam (ABSC) is a rare wild edible macrofungus that grows underground at high altitudes. Herein, intracellular phenolic acids-rich fractions (IPA) were extracted from ABSC ZJU-CDMA-12, and the structural characterization and anti-hypoxia activity of IPA on PC12 cells were elucidated as well. The results of HPLC-Q-TOF-MS illustrated that five kinds of IPA were isolated from ABSC, including (−)-epicatechin gallate, arabelline, yunnaneic acid <span style="font-variant: small-caps;">D</span>, 2′-<i>O</i>-p-hydroxybenzoyl-6′-<i>O</i>-trans-caffeoylgardoside,4′-<i>O</i>-methylgallocatechin-(4->8)-4′-<i>O</i>-methylepigallocatechin. IPA extracted from ABSC proved to show anti-hypoxia activity on hypoxia-damaged PC12 cells. Hypoxia enhanced reactive oxygen species (ROS) generation and reduced the mitochondrial membrane potential (ΔΨm) in PC12 cells, resulting in the inhibition of survival and induction of apoptosis in PC12 cells. Measurements of 100 μg/mL and 250 μg/mL IPA could significantly reduce hypoxia-induced damage in PC12 cells by decreasing overproduced intracellular ROS, improving ΔΨm, and reducing cell apoptosis rate. Our findings indicated that the IPA from ABSC potentially could be used as novel bioactive components applied to anti-hypoxia functional foods or medicines. |
| first_indexed | 2024-03-10T15:27:32Z |
| format | Article |
| id | doaj.art-674b4de809544233b8a17f2ede3ae31a |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-10T15:27:32Z |
| publishDate | 2020-10-01 |
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| series | Molecules |
| spelling | doaj.art-674b4de809544233b8a17f2ede3ae31a2023-11-20T17:53:21ZengMDPI AGMolecules1420-30492020-10-012520484510.3390/molecules25204845Phenolic Acids-Rich Fractions from <i>Agaricus bitorguis</i> (Quél.) Sacc. Chaidam ZJU-CDMA-12 Mycelia Modulate Hypoxic Stress on Hypoxia-Damaged PC12 CellsHongyun Lu0Zhihua Jiao1Yingchun Jiao2Wei Wang3Qihe Chen4Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, ChinaDepartment of Cell Biology, School of Medicine, Johns Hopkins University, Baltimore, MD 21205, USAAgriculture and Animal Husbandry College, Qinghai University, Xining 810016, ChinaInstitute of Quality and Standard for Agriculture Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaDepartment of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, ChinaHypoxia is a common pathological process in various clinical diseases. However, there is still a lack of effective anti-hypoxia active substances. <i>Agaricus bitorguis</i> (Quél.) Sacc Chaidam (ABSC) is a rare wild edible macrofungus that grows underground at high altitudes. Herein, intracellular phenolic acids-rich fractions (IPA) were extracted from ABSC ZJU-CDMA-12, and the structural characterization and anti-hypoxia activity of IPA on PC12 cells were elucidated as well. The results of HPLC-Q-TOF-MS illustrated that five kinds of IPA were isolated from ABSC, including (−)-epicatechin gallate, arabelline, yunnaneic acid <span style="font-variant: small-caps;">D</span>, 2′-<i>O</i>-p-hydroxybenzoyl-6′-<i>O</i>-trans-caffeoylgardoside,4′-<i>O</i>-methylgallocatechin-(4->8)-4′-<i>O</i>-methylepigallocatechin. IPA extracted from ABSC proved to show anti-hypoxia activity on hypoxia-damaged PC12 cells. Hypoxia enhanced reactive oxygen species (ROS) generation and reduced the mitochondrial membrane potential (ΔΨm) in PC12 cells, resulting in the inhibition of survival and induction of apoptosis in PC12 cells. Measurements of 100 μg/mL and 250 μg/mL IPA could significantly reduce hypoxia-induced damage in PC12 cells by decreasing overproduced intracellular ROS, improving ΔΨm, and reducing cell apoptosis rate. Our findings indicated that the IPA from ABSC potentially could be used as novel bioactive components applied to anti-hypoxia functional foods or medicines.https://www.mdpi.com/1420-3049/25/20/4845intracellular phenolic acids<i>Agaricus bitorquis</i> (QuéL.) Sacc. Chaidamstructure characterizationanti-hypoxia activity |
| spellingShingle | Hongyun Lu Zhihua Jiao Yingchun Jiao Wei Wang Qihe Chen Phenolic Acids-Rich Fractions from <i>Agaricus bitorguis</i> (Quél.) Sacc. Chaidam ZJU-CDMA-12 Mycelia Modulate Hypoxic Stress on Hypoxia-Damaged PC12 Cells Molecules intracellular phenolic acids <i>Agaricus bitorquis</i> (QuéL.) Sacc. Chaidam structure characterization anti-hypoxia activity |
| title | Phenolic Acids-Rich Fractions from <i>Agaricus bitorguis</i> (Quél.) Sacc. Chaidam ZJU-CDMA-12 Mycelia Modulate Hypoxic Stress on Hypoxia-Damaged PC12 Cells |
| title_full | Phenolic Acids-Rich Fractions from <i>Agaricus bitorguis</i> (Quél.) Sacc. Chaidam ZJU-CDMA-12 Mycelia Modulate Hypoxic Stress on Hypoxia-Damaged PC12 Cells |
| title_fullStr | Phenolic Acids-Rich Fractions from <i>Agaricus bitorguis</i> (Quél.) Sacc. Chaidam ZJU-CDMA-12 Mycelia Modulate Hypoxic Stress on Hypoxia-Damaged PC12 Cells |
| title_full_unstemmed | Phenolic Acids-Rich Fractions from <i>Agaricus bitorguis</i> (Quél.) Sacc. Chaidam ZJU-CDMA-12 Mycelia Modulate Hypoxic Stress on Hypoxia-Damaged PC12 Cells |
| title_short | Phenolic Acids-Rich Fractions from <i>Agaricus bitorguis</i> (Quél.) Sacc. Chaidam ZJU-CDMA-12 Mycelia Modulate Hypoxic Stress on Hypoxia-Damaged PC12 Cells |
| title_sort | phenolic acids rich fractions from i agaricus bitorguis i quel sacc chaidam zju cdma 12 mycelia modulate hypoxic stress on hypoxia damaged pc12 cells |
| topic | intracellular phenolic acids <i>Agaricus bitorquis</i> (QuéL.) Sacc. Chaidam structure characterization anti-hypoxia activity |
| url | https://www.mdpi.com/1420-3049/25/20/4845 |
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