Immunochemical, biochemical and pathogenic properties of fluidal and intermediate strains of Clavibacter michiganensis subsp. sepedonicus
Clavibacter michiganensis subsp. sepedonicus (Cms), causing ring rot of potato, is a quarantine bacterium. According to colony morphology, Cms occurs mostly as fluidal (smooth), but sometime as intermediate (rough, i.e. less fluidal) variants, too. Commercial monoclonal antibodies (Agdia, USA) were...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2002-12-01
|
| Series: | Plant Protection Science |
| Subjects: | |
| Online Access: | https://pps.agriculturejournals.cz/artkey/pps-200211-0014_immunochemical-biochemical-and-pathogenic-properties-of-fluidal-and-intermediate-strains-of-clavibacter-michig.php |
| _version_ | 1828007871113068544 |
|---|---|
| author | B. Kokošková R. Jeřabková |
| author_facet | B. Kokošková R. Jeřabková |
| author_sort | B. Kokošková |
| collection | DOAJ |
| description | Clavibacter michiganensis subsp. sepedonicus (Cms), causing ring rot of potato, is a quarantine bacterium. According to colony morphology, Cms occurs mostly as fluidal (smooth), but sometime as intermediate (rough, i.e. less fluidal) variants, too. Commercial monoclonal antibodies (Agdia, USA) were used for determination of 40 Cms strains representing both forms. All Cms strains were reliably identified by IFAS, but atypical cells were sometime recorded in population of intermediate strains. The fluidal Cms strains were more reliably identified using DAS-ELISA and the Biolog GP MicroPlate SystemTM than intermediate strains. The intermediate Cms strains had decreased metabolic activity compared with fluidal strains and that is why they were identified only to the genera or to the species level or not identified. The differences among fluidal and intermediate Cms strains were recorded also in bioassay on eggplants. The intermediate Cms strains caused atypical or no symptoms with comparison to fluidal strains. |
| first_indexed | 2024-04-10T08:16:15Z |
| format | Article |
| id | doaj.art-674dfd5502624ae69a8d087f832a146d |
| institution | Directory Open Access Journal |
| issn | 1212-2580 1805-9341 |
| language | English |
| last_indexed | 2024-04-10T08:16:15Z |
| publishDate | 2002-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant Protection Science |
| spelling | doaj.art-674dfd5502624ae69a8d087f832a146d2023-02-23T03:43:40ZengCzech Academy of Agricultural SciencesPlant Protection Science1212-25801805-93412002-12-0138SI 2 - 6th Conf EFPP29129310.17221/10471-PPSpps-200211-0014Immunochemical, biochemical and pathogenic properties of fluidal and intermediate strains of Clavibacter michiganensis subsp. sepedonicusB. Kokošková0R. Jeřabková1Division of Plant Medicine, Research Institute of Crop Production, 161 06 Prague-Ruzyně, Czech Republic *Division of Plant Medicine, Research Institute of Crop Production, 161 06 Prague-Ruzyně, Czech Republic *Clavibacter michiganensis subsp. sepedonicus (Cms), causing ring rot of potato, is a quarantine bacterium. According to colony morphology, Cms occurs mostly as fluidal (smooth), but sometime as intermediate (rough, i.e. less fluidal) variants, too. Commercial monoclonal antibodies (Agdia, USA) were used for determination of 40 Cms strains representing both forms. All Cms strains were reliably identified by IFAS, but atypical cells were sometime recorded in population of intermediate strains. The fluidal Cms strains were more reliably identified using DAS-ELISA and the Biolog GP MicroPlate SystemTM than intermediate strains. The intermediate Cms strains had decreased metabolic activity compared with fluidal strains and that is why they were identified only to the genera or to the species level or not identified. The differences among fluidal and intermediate Cms strains were recorded also in bioassay on eggplants. The intermediate Cms strains caused atypical or no symptoms with comparison to fluidal strains.https://pps.agriculturejournals.cz/artkey/pps-200211-0014_immunochemical-biochemical-and-pathogenic-properties-of-fluidal-and-intermediate-strains-of-clavibacter-michig.phpclavibacter michiganensis subsp. sepedonicusring rot of potatoelisaifasbiologbioassay |
| spellingShingle | B. Kokošková R. Jeřabková Immunochemical, biochemical and pathogenic properties of fluidal and intermediate strains of Clavibacter michiganensis subsp. sepedonicus Plant Protection Science clavibacter michiganensis subsp. sepedonicus ring rot of potato elisa ifas biolog bioassay |
| title | Immunochemical, biochemical and pathogenic properties of fluidal and intermediate strains of Clavibacter michiganensis subsp. sepedonicus |
| title_full | Immunochemical, biochemical and pathogenic properties of fluidal and intermediate strains of Clavibacter michiganensis subsp. sepedonicus |
| title_fullStr | Immunochemical, biochemical and pathogenic properties of fluidal and intermediate strains of Clavibacter michiganensis subsp. sepedonicus |
| title_full_unstemmed | Immunochemical, biochemical and pathogenic properties of fluidal and intermediate strains of Clavibacter michiganensis subsp. sepedonicus |
| title_short | Immunochemical, biochemical and pathogenic properties of fluidal and intermediate strains of Clavibacter michiganensis subsp. sepedonicus |
| title_sort | immunochemical biochemical and pathogenic properties of fluidal and intermediate strains of clavibacter michiganensis subsp sepedonicus |
| topic | clavibacter michiganensis subsp. sepedonicus ring rot of potato elisa ifas biolog bioassay |
| url | https://pps.agriculturejournals.cz/artkey/pps-200211-0014_immunochemical-biochemical-and-pathogenic-properties-of-fluidal-and-intermediate-strains-of-clavibacter-michig.php |
| work_keys_str_mv | AT bkokoskova immunochemicalbiochemicalandpathogenicpropertiesoffluidalandintermediatestrainsofclavibactermichiganensissubspsepedonicus AT rjerabkova immunochemicalbiochemicalandpathogenicpropertiesoffluidalandintermediatestrainsofclavibactermichiganensissubspsepedonicus |