Memory Recovery Effect of a New Bioactive Innovative Combination in Rats with Experimental Dementia
Alzheimer’s disease manifests as a complex pathological condition, with neuroinflammation, oxidative stress and cholinergic dysfunction being a few of the many pathological changes. Due to the complexity of the disease, current therapeutic strategies aim at a multitargeted approach, often relying on...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/12/12/2050 |
| _version_ | 1827575833412239360 |
|---|---|
| author | Lyubka Tancheva Reni Kalfin Borislav Minchev Diamara Uzunova Krasimira Tasheva Elina Tsvetanova Almira Georgieva Albena Alexandrova Miroslava Stefanova Ayten Solak Maria Lazarova Yordan Hodzhev Valya Grigorova Dobri Yarkov Polina Petkova-Kirova |
| author_facet | Lyubka Tancheva Reni Kalfin Borislav Minchev Diamara Uzunova Krasimira Tasheva Elina Tsvetanova Almira Georgieva Albena Alexandrova Miroslava Stefanova Ayten Solak Maria Lazarova Yordan Hodzhev Valya Grigorova Dobri Yarkov Polina Petkova-Kirova |
| author_sort | Lyubka Tancheva |
| collection | DOAJ |
| description | Alzheimer’s disease manifests as a complex pathological condition, with neuroinflammation, oxidative stress and cholinergic dysfunction being a few of the many pathological changes. Due to the complexity of the disease, current therapeutic strategies aim at a multitargeted approach, often relying on a combination of substances with versatile and complementary effects. In the present study, a unique combination of α-lipoic acid, citicoline, extracts of leaves from olive tree and green tea, vitamin D3, selenium and an immune-supporting complex was tested in scopolamine-induced dementia in rats. Using behavioral and biochemical methods, we assessed the effects of the combination on learning and memory, and elucidated the mechanisms of these effects. Our results showed that, compared to its components, the experimental combination was most efficient in improving short- and long-term memory as assessed by the step-through method as well as spatial memory as assessed by T-maze and Barnes maze underlined by decreases in AChE activity (<i>p</i> < 0.05) and LPO (<i>p</i> < 0.001), increases in SOD activity in the cortex (<i>p</i> < 0.05) and increases in catalase (<i>p</i> < 0.05) and GPx (<i>p</i> < 0.01) activities and BDNF (<i>p</i> < 0.001) and pCREB (<i>p</i> < 0.05) levels in the hippocampus. No significant histopathological changes or blood parameter changes were detected, making the experimental combination an effective and safe candidate in a multitargeted treatment of AD. |
| first_indexed | 2024-03-08T21:02:34Z |
| format | Article |
| id | doaj.art-674eea6c94ec4f5a85308a62edce9f1b |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-08T21:02:34Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-674eea6c94ec4f5a85308a62edce9f1b2023-12-22T13:48:21ZengMDPI AGAntioxidants2076-39212023-11-011212205010.3390/antiox12122050Memory Recovery Effect of a New Bioactive Innovative Combination in Rats with Experimental DementiaLyubka Tancheva0Reni Kalfin1Borislav Minchev2Diamara Uzunova3Krasimira Tasheva4Elina Tsvetanova5Almira Georgieva6Albena Alexandrova7Miroslava Stefanova8Ayten Solak9Maria Lazarova10Yordan Hodzhev11Valya Grigorova12Dobri Yarkov13Polina Petkova-Kirova14Institute of Neurobiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. 23, 1113 Sofia, BulgariaInstitute of Neurobiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. 23, 1113 Sofia, BulgariaInstitute of Neurobiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. 23, 1113 Sofia, BulgariaInstitute of Neurobiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. 23, 1113 Sofia, BulgariaInstitute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. 21, 1113 Sofia, BulgariaInstitute of Neurobiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. 23, 1113 Sofia, BulgariaInstitute of Neurobiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. 23, 1113 Sofia, BulgariaInstitute of Neurobiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. 23, 1113 Sofia, BulgariaInstitute of Neurobiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. 23, 1113 Sofia, BulgariaInstitute of Neurobiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. 23, 1113 Sofia, BulgariaInstitute of Neurobiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. 23, 1113 Sofia, BulgariaNational Center of Infectious and Parasitic Diseases, Yanko Sakazov Blvd 26, 1504 Sofia, BulgariaInstitute of Neurobiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. 23, 1113 Sofia, BulgariaFaculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, BulgariaInstitute of Neurobiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. 23, 1113 Sofia, BulgariaAlzheimer’s disease manifests as a complex pathological condition, with neuroinflammation, oxidative stress and cholinergic dysfunction being a few of the many pathological changes. Due to the complexity of the disease, current therapeutic strategies aim at a multitargeted approach, often relying on a combination of substances with versatile and complementary effects. In the present study, a unique combination of α-lipoic acid, citicoline, extracts of leaves from olive tree and green tea, vitamin D3, selenium and an immune-supporting complex was tested in scopolamine-induced dementia in rats. Using behavioral and biochemical methods, we assessed the effects of the combination on learning and memory, and elucidated the mechanisms of these effects. Our results showed that, compared to its components, the experimental combination was most efficient in improving short- and long-term memory as assessed by the step-through method as well as spatial memory as assessed by T-maze and Barnes maze underlined by decreases in AChE activity (<i>p</i> < 0.05) and LPO (<i>p</i> < 0.001), increases in SOD activity in the cortex (<i>p</i> < 0.05) and increases in catalase (<i>p</i> < 0.05) and GPx (<i>p</i> < 0.01) activities and BDNF (<i>p</i> < 0.001) and pCREB (<i>p</i> < 0.05) levels in the hippocampus. No significant histopathological changes or blood parameter changes were detected, making the experimental combination an effective and safe candidate in a multitargeted treatment of AD.https://www.mdpi.com/2076-3921/12/12/2050Alzheimer’s diseaseα-lipoic acidciticolineextract of leaves green teaextract of leaves olive treevitamin D<sub>3</sub> |
| spellingShingle | Lyubka Tancheva Reni Kalfin Borislav Minchev Diamara Uzunova Krasimira Tasheva Elina Tsvetanova Almira Georgieva Albena Alexandrova Miroslava Stefanova Ayten Solak Maria Lazarova Yordan Hodzhev Valya Grigorova Dobri Yarkov Polina Petkova-Kirova Memory Recovery Effect of a New Bioactive Innovative Combination in Rats with Experimental Dementia Antioxidants Alzheimer’s disease α-lipoic acid citicoline extract of leaves green tea extract of leaves olive tree vitamin D<sub>3</sub> |
| title | Memory Recovery Effect of a New Bioactive Innovative Combination in Rats with Experimental Dementia |
| title_full | Memory Recovery Effect of a New Bioactive Innovative Combination in Rats with Experimental Dementia |
| title_fullStr | Memory Recovery Effect of a New Bioactive Innovative Combination in Rats with Experimental Dementia |
| title_full_unstemmed | Memory Recovery Effect of a New Bioactive Innovative Combination in Rats with Experimental Dementia |
| title_short | Memory Recovery Effect of a New Bioactive Innovative Combination in Rats with Experimental Dementia |
| title_sort | memory recovery effect of a new bioactive innovative combination in rats with experimental dementia |
| topic | Alzheimer’s disease α-lipoic acid citicoline extract of leaves green tea extract of leaves olive tree vitamin D<sub>3</sub> |
| url | https://www.mdpi.com/2076-3921/12/12/2050 |
| work_keys_str_mv | AT lyubkatancheva memoryrecoveryeffectofanewbioactiveinnovativecombinationinratswithexperimentaldementia AT renikalfin memoryrecoveryeffectofanewbioactiveinnovativecombinationinratswithexperimentaldementia AT borislavminchev memoryrecoveryeffectofanewbioactiveinnovativecombinationinratswithexperimentaldementia AT diamarauzunova memoryrecoveryeffectofanewbioactiveinnovativecombinationinratswithexperimentaldementia AT krasimiratasheva memoryrecoveryeffectofanewbioactiveinnovativecombinationinratswithexperimentaldementia AT elinatsvetanova memoryrecoveryeffectofanewbioactiveinnovativecombinationinratswithexperimentaldementia AT almirageorgieva memoryrecoveryeffectofanewbioactiveinnovativecombinationinratswithexperimentaldementia AT albenaalexandrova memoryrecoveryeffectofanewbioactiveinnovativecombinationinratswithexperimentaldementia AT miroslavastefanova memoryrecoveryeffectofanewbioactiveinnovativecombinationinratswithexperimentaldementia AT aytensolak memoryrecoveryeffectofanewbioactiveinnovativecombinationinratswithexperimentaldementia AT marialazarova memoryrecoveryeffectofanewbioactiveinnovativecombinationinratswithexperimentaldementia AT yordanhodzhev memoryrecoveryeffectofanewbioactiveinnovativecombinationinratswithexperimentaldementia AT valyagrigorova memoryrecoveryeffectofanewbioactiveinnovativecombinationinratswithexperimentaldementia AT dobriyarkov memoryrecoveryeffectofanewbioactiveinnovativecombinationinratswithexperimentaldementia AT polinapetkovakirova memoryrecoveryeffectofanewbioactiveinnovativecombinationinratswithexperimentaldementia |