Phytochemical screening and antibacterial activity of ethnomedicinal plants from Gayo Lues Highland, Indonesia
Context: Investigations of phytochemical screening and antibacterial activity were carried out on traditional medicinal plants collected in the highlands of Gayo Lues, Aceh Province, at an elevation of 1,000 meters above sea level (m.a.s.l.). Aims: To evaluate the antibacterial activity of plants...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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GarVal Editorial Ltda.
2023-01-01
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| Series: | Journal of Pharmacy & Pharmacognosy Research |
| Subjects: | |
| Online Access: | https://jppres.com/jppres/pdf/vol11/jppres22.1526_11.1.117.pdf |
| _version_ | 1811168467950239744 |
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| author | Hawa Purnama Celala Ary Cane Musri Musman Mustanir Yahya Nurdin Saidi Darusman Darusman Muhammad Nanda Diva Rayyan Rizki Kana Puspita |
| author_facet | Hawa Purnama Celala Ary Cane Musri Musman Mustanir Yahya Nurdin Saidi Darusman Darusman Muhammad Nanda Diva Rayyan Rizki Kana Puspita |
| author_sort | Hawa Purnama Celala Ary Cane |
| collection | DOAJ |
| description | Context: Investigations of phytochemical screening and antibacterial activity were carried out on traditional medicinal plants collected in the highlands of Gayo Lues, Aceh Province, at an elevation of 1,000 meters above sea level (m.a.s.l.).
Aims: To evaluate the antibacterial activity of plants chosen based on the interview results with the traditional healers (n = 5) in Gayo Lues.
Methods: Ethanolic maceration was performed on the 12 identified ethnomedicinal plants and qualitatively screened for the phytochemical contents. Antibacterial activities against Escherichia coli ATCC 25922 and Staphylococcus aureus were tested for each extract based on the disc diffusion method, and MIC was determined using cephazolin as a reference drug.
Results: The phytochemical screening of twelve plant species showed secondary metabolites class steroids, terpenoids, flavonoids, phenols, alkaloids, and saponins. Evaluation of the antibacterial activity of the extract (10 mg/mL) yielded inhibition zone ranges of 9.8 ± 0.26 to 21.87 ± 0.47 mm for E. coli and 8.93 ± 0.9 to 23.97 ± 0.68 for S. aureus. The ethanolic extract of Garcinia macrophylla Mart stem barks showed the highest antibacterial activity, where at the lowest concentration (1.25 mg/mL), the inhibition zones were found to be 19.2 ± 0.61 and 20.72 ± 0.44 mm for E. coli and S. aureus, respectively. The MIC was found to be 1.25 mg/mL.
Conclusions: This study concludes that the twelve plant species are worthy of further investigation for novel antibacterial agent exploration. |
| first_indexed | 2024-04-10T16:26:51Z |
| format | Article |
| id | doaj.art-674f41bec8a849be9cd915841b81fa78 |
| institution | Directory Open Access Journal |
| issn | 0719-4250 |
| language | English |
| last_indexed | 2024-04-10T16:26:51Z |
| publishDate | 2023-01-01 |
| publisher | GarVal Editorial Ltda. |
| record_format | Article |
| series | Journal of Pharmacy & Pharmacognosy Research |
| spelling | doaj.art-674f41bec8a849be9cd915841b81fa782023-02-09T03:14:25ZengGarVal Editorial Ltda.Journal of Pharmacy & Pharmacognosy Research0719-42502023-01-01111117128https://doi.org/10.56499/jppres22.1526_11.1.117Phytochemical screening and antibacterial activity of ethnomedicinal plants from Gayo Lues Highland, IndonesiaHawa Purnama Celala Ary Cane0Musri Musman1Mustanir Yahya2Nurdin Saidi3Darusman Darusman4Muhammad Nanda5Diva Rayyan Rizki6Kana Puspita7Department of Chemistry, Institut Teknologi Sumatera, Lampung Selatan 35365, Indonesia.Department of Chemistry Education, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia.Department of Chemistry, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia.Department of Chemistry, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia.Department of Soil Science, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia.Department of Marine Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia.Medical Research Unit, School of Medicine, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia. Innovative Sustainability Lab, PT. Biham Riset dan Edukasi, Banda Aceh 23243, Indonesia.Department of Chemistry Education, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia.Context: Investigations of phytochemical screening and antibacterial activity were carried out on traditional medicinal plants collected in the highlands of Gayo Lues, Aceh Province, at an elevation of 1,000 meters above sea level (m.a.s.l.). Aims: To evaluate the antibacterial activity of plants chosen based on the interview results with the traditional healers (n = 5) in Gayo Lues. Methods: Ethanolic maceration was performed on the 12 identified ethnomedicinal plants and qualitatively screened for the phytochemical contents. Antibacterial activities against Escherichia coli ATCC 25922 and Staphylococcus aureus were tested for each extract based on the disc diffusion method, and MIC was determined using cephazolin as a reference drug. Results: The phytochemical screening of twelve plant species showed secondary metabolites class steroids, terpenoids, flavonoids, phenols, alkaloids, and saponins. Evaluation of the antibacterial activity of the extract (10 mg/mL) yielded inhibition zone ranges of 9.8 ± 0.26 to 21.87 ± 0.47 mm for E. coli and 8.93 ± 0.9 to 23.97 ± 0.68 for S. aureus. The ethanolic extract of Garcinia macrophylla Mart stem barks showed the highest antibacterial activity, where at the lowest concentration (1.25 mg/mL), the inhibition zones were found to be 19.2 ± 0.61 and 20.72 ± 0.44 mm for E. coli and S. aureus, respectively. The MIC was found to be 1.25 mg/mL. Conclusions: This study concludes that the twelve plant species are worthy of further investigation for novel antibacterial agent exploration.https://jppres.com/jppres/pdf/vol11/jppres22.1526_11.1.117.pdfantibacterialescherichia coliethnomedicinal plantgarcinia macrophyllaphytochemicalstaphylococcus aureus |
| spellingShingle | Hawa Purnama Celala Ary Cane Musri Musman Mustanir Yahya Nurdin Saidi Darusman Darusman Muhammad Nanda Diva Rayyan Rizki Kana Puspita Phytochemical screening and antibacterial activity of ethnomedicinal plants from Gayo Lues Highland, Indonesia Journal of Pharmacy & Pharmacognosy Research antibacterial escherichia coli ethnomedicinal plant garcinia macrophylla phytochemical staphylococcus aureus |
| title | Phytochemical screening and antibacterial activity of ethnomedicinal plants from Gayo Lues Highland, Indonesia |
| title_full | Phytochemical screening and antibacterial activity of ethnomedicinal plants from Gayo Lues Highland, Indonesia |
| title_fullStr | Phytochemical screening and antibacterial activity of ethnomedicinal plants from Gayo Lues Highland, Indonesia |
| title_full_unstemmed | Phytochemical screening and antibacterial activity of ethnomedicinal plants from Gayo Lues Highland, Indonesia |
| title_short | Phytochemical screening and antibacterial activity of ethnomedicinal plants from Gayo Lues Highland, Indonesia |
| title_sort | phytochemical screening and antibacterial activity of ethnomedicinal plants from gayo lues highland indonesia |
| topic | antibacterial escherichia coli ethnomedicinal plant garcinia macrophylla phytochemical staphylococcus aureus |
| url | https://jppres.com/jppres/pdf/vol11/jppres22.1526_11.1.117.pdf |
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