Generation of viable blastocysts from discarded human cleavage embryos
Abstract Background While a relationship between embryo morphology, developmental ability, and genetic integrity exists, the selection of embryos with higher implantation potential remains a major challenge in assisted reproductive technology (ART). This study investigated blastocyst developmental c...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-06-01
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| Series: | Middle East Fertility Society Journal |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s43043-020-00028-1 |
| _version_ | 1818459764616593408 |
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| author | Fatemeh Taheri Mohammad Ali Khalili Seyed Mehdi Kalantar Farzaneh Fesahat Fatemeh Montazeri Maria Grazia Palmerini Bryan Woodward |
| author_facet | Fatemeh Taheri Mohammad Ali Khalili Seyed Mehdi Kalantar Farzaneh Fesahat Fatemeh Montazeri Maria Grazia Palmerini Bryan Woodward |
| author_sort | Fatemeh Taheri |
| collection | DOAJ |
| description | Abstract Background While a relationship between embryo morphology, developmental ability, and genetic integrity exists, the selection of embryos with higher implantation potential remains a major challenge in assisted reproductive technology (ART). This study investigated blastocyst developmental competence and euploidy status in human embryos that had been classed as too poor quality to transfer (ET) or cryopreserve at the cleavage stage. Embryos were divided into three groups. Group 1 (n = 41) included good quality embryos from candidates of preimplantation genetic testing for aneuploidy (PGT-A). Groups II and III were the “rejected” supernumerary embryos, defined as suboptimal for ET or vitrification after morphological examination, with embryos randomly divided between the groups. Group II embryos (n = 31) were cultured up to the day 3 cleavage stage, when they were biopsied and fixed. Group III embryos (n = 27) were cultured up to the day 5 blastocyst stage, when they were evaluated for morphology and chromosomal status. Chromosomal status in all groups was assessed by multi-color fluorescence in situ hybridization (FISH) for chromosomes 13, 18, 21, X, and Y. Results Euploidy rates in groups I, II, and III were 56.1%, 38.7%, and 55.5 %, respectively. Among the blastocysts that developed from “rejected” embryos, 59.3% were classed as good quality. The most frequent chromosomal aneuploidy was related to the sex chromosome (22.2%). The mosaicism rate was not significantly different between the group II and III embryos (25.8% vs. 37.0%, p = 0.28). Conclusion In conclusion, surplus poor-quality embryos rejected from clinical utilization at the cleavage stage may develop into viable blastocysts with normal chromosomal status for at least 5 chromosomes. Recovery of euploidy during poor-quality embryo transition from cleavage stage to blastocyst could provide an alternative choice for ET. |
| first_indexed | 2024-12-14T23:19:32Z |
| format | Article |
| id | doaj.art-6752bc001aed40029e5360f729b001af |
| institution | Directory Open Access Journal |
| issn | 2090-3251 |
| language | English |
| last_indexed | 2024-12-14T23:19:32Z |
| publishDate | 2020-06-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Middle East Fertility Society Journal |
| spelling | doaj.art-6752bc001aed40029e5360f729b001af2022-12-21T22:44:00ZengSpringerOpenMiddle East Fertility Society Journal2090-32512020-06-012511910.1186/s43043-020-00028-1Generation of viable blastocysts from discarded human cleavage embryosFatemeh Taheri0Mohammad Ali Khalili1Seyed Mehdi Kalantar2Farzaneh Fesahat3Fatemeh Montazeri4Maria Grazia Palmerini5Bryan Woodward6Department of Reproductive Biology, Yazd Institute for Reproductive Sciences, Shahid Sadoughi University of Medical SciencesDepartment of Reproductive Biology, Yazd Institute for Reproductive Sciences, Shahid Sadoughi University of Medical SciencesAbortion Research Center, Yazd Institute for Reproductive Sciences, Shahid Sadoughi University of Medical SciencesReproductive Immunology Research Center, Shahid Sadoughi University of Medical SciencesAbortion Research Center, Yazd Institute for Reproductive Sciences, Shahid Sadoughi University of Medical SciencesDepartment of Life, Health and Environmental Sciences, University of L’AquilaX &Y FertilityAbstract Background While a relationship between embryo morphology, developmental ability, and genetic integrity exists, the selection of embryos with higher implantation potential remains a major challenge in assisted reproductive technology (ART). This study investigated blastocyst developmental competence and euploidy status in human embryos that had been classed as too poor quality to transfer (ET) or cryopreserve at the cleavage stage. Embryos were divided into three groups. Group 1 (n = 41) included good quality embryos from candidates of preimplantation genetic testing for aneuploidy (PGT-A). Groups II and III were the “rejected” supernumerary embryos, defined as suboptimal for ET or vitrification after morphological examination, with embryos randomly divided between the groups. Group II embryos (n = 31) were cultured up to the day 3 cleavage stage, when they were biopsied and fixed. Group III embryos (n = 27) were cultured up to the day 5 blastocyst stage, when they were evaluated for morphology and chromosomal status. Chromosomal status in all groups was assessed by multi-color fluorescence in situ hybridization (FISH) for chromosomes 13, 18, 21, X, and Y. Results Euploidy rates in groups I, II, and III were 56.1%, 38.7%, and 55.5 %, respectively. Among the blastocysts that developed from “rejected” embryos, 59.3% were classed as good quality. The most frequent chromosomal aneuploidy was related to the sex chromosome (22.2%). The mosaicism rate was not significantly different between the group II and III embryos (25.8% vs. 37.0%, p = 0.28). Conclusion In conclusion, surplus poor-quality embryos rejected from clinical utilization at the cleavage stage may develop into viable blastocysts with normal chromosomal status for at least 5 chromosomes. Recovery of euploidy during poor-quality embryo transition from cleavage stage to blastocyst could provide an alternative choice for ET.http://link.springer.com/article/10.1186/s43043-020-00028-1BlastocystPoor-quality embryoFISHAneuploidy |
| spellingShingle | Fatemeh Taheri Mohammad Ali Khalili Seyed Mehdi Kalantar Farzaneh Fesahat Fatemeh Montazeri Maria Grazia Palmerini Bryan Woodward Generation of viable blastocysts from discarded human cleavage embryos Middle East Fertility Society Journal Blastocyst Poor-quality embryo FISH Aneuploidy |
| title | Generation of viable blastocysts from discarded human cleavage embryos |
| title_full | Generation of viable blastocysts from discarded human cleavage embryos |
| title_fullStr | Generation of viable blastocysts from discarded human cleavage embryos |
| title_full_unstemmed | Generation of viable blastocysts from discarded human cleavage embryos |
| title_short | Generation of viable blastocysts from discarded human cleavage embryos |
| title_sort | generation of viable blastocysts from discarded human cleavage embryos |
| topic | Blastocyst Poor-quality embryo FISH Aneuploidy |
| url | http://link.springer.com/article/10.1186/s43043-020-00028-1 |
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