Phytopathological and biochemical impacts of Trichoderma harzianum and certain plant resistance inducers on faba bean root rot disease
Abstract Background Faba bean attacked by soil-borne pathogens causing root rot disease. This disease has serious damage to both plant stand and produced yield. The present study aimed to evaluate effectiveness of the bioagents; Trichoderma harzianum and some plant resistance inducers as fungicide a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-06-01
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| Series: | Egyptian Journal of Biological Pest Control |
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| Online Access: | https://doi.org/10.1186/s41938-023-00709-9 |
| _version_ | 1797801240218828800 |
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| author | Hassan Mohamed Masoud Allam Arafat Megahed Mohamed Salah Eldin Helmy Mahmoud Abdel-Aziz Ibrahim Nehal Samy El-Mougy Mokhtar Mohamed Abdel-Kader |
| author_facet | Hassan Mohamed Masoud Allam Arafat Megahed Mohamed Salah Eldin Helmy Mahmoud Abdel-Aziz Ibrahim Nehal Samy El-Mougy Mokhtar Mohamed Abdel-Kader |
| author_sort | Hassan Mohamed Masoud |
| collection | DOAJ |
| description | Abstract Background Faba bean attacked by soil-borne pathogens causing root rot disease. This disease has serious damage to both plant stand and produced yield. The present study aimed to evaluate effectiveness of the bioagents; Trichoderma harzianum and some plant resistance inducers as fungicide alternatives against root rot disease incidence at both pre- and post-emergence growth stages. Results Under open greenhouse conditions, the incidence of faba bean root rot in pre- and post-emergence growth phases was considerably reduced by using six inorganic salts and five antioxidants individually or combining with each other or with the bio-stimulator T. harzianum that exceeded the used fungicide, Rhizolex-T. Application of enervit agitated the highest significant defensive impact during pre-emergence stage versus root rot incidence (5.0%), followed by calcium sulfate and [cysteine + T. harzianum] (6.7%). At post-emergence stage, majority of the treatments completely suppressed (100.0%) root rot incidence, except vitamax plus and the fungicide (Rizolex-T) which expressed by 91.7 and 18.8%, respectively. Duplicate irrigations of 23 treatments after faba bean dressing improved the synthesis of different protein contents with the 2nd of which enhanced higher protein contents than the 1st one, except [T. harzianum + vitamin E + vitamin C + enervit + selenium + vitamax plus], [T. harzianum + vitamax plus] and cysteine. Disodium phosphate induced the highest catalase (CAT) activity (1820.8 and 1677.2 U/g FWt) after both irrigations. [T. harzianum + vitamax plus] and vitamin E induced the highest peroxidase (POD) activity 217.4 and 356.9 U/g FWt after 1st and 2nd irrigations, respectively. Disodium phosphate and [T. harzianum + vitamin E + vitamin C + enervit + selenium + vitamax plus] induced the highest chitinase (CHIA) activity 52.8 and 54.4 U/g FWt after 1st and 2nd irrigations, respectively. Application of disodium phosphate, calcium sulfate, potassium metabisulfite, sodium sulfate, cysteine, [cysteine + potash alum], enervit, vitamin E, [vitamin E + vitamin C + enervit + selenium + vitamax plus], [T. harzianum + enervit], [T. harzianum + selenium], [T. harzianum + vitamin E], [T. harzianum + vitamin E + vitamin C + enervit + selenium + vitamax plus] and vitamin C stimulated the formation of new protein bands on SDS-PAGE after the 2nd irrigation treatment. Conclusions Such treatments are considered good and environmentally safe alternatives against root diseases for getting rid of the negative effects of fungicides. |
| first_indexed | 2024-03-13T04:47:27Z |
| format | Article |
| id | doaj.art-6754f6b825344ef68b9c00123a58a7bb |
| institution | Directory Open Access Journal |
| issn | 2536-9342 |
| language | English |
| last_indexed | 2024-03-13T04:47:27Z |
| publishDate | 2023-06-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Biological Pest Control |
| spelling | doaj.art-6754f6b825344ef68b9c00123a58a7bb2023-06-18T11:24:29ZengSpringerOpenEgyptian Journal of Biological Pest Control2536-93422023-06-0133111810.1186/s41938-023-00709-9Phytopathological and biochemical impacts of Trichoderma harzianum and certain plant resistance inducers on faba bean root rot diseaseHassan Mohamed Masoud0Allam Arafat Megahed1Mohamed Salah Eldin Helmy2Mahmoud Abdel-Aziz Ibrahim3Nehal Samy El-Mougy4Mokhtar Mohamed Abdel-Kader5Molecular Biology Department, National Research CentreAgricultural Botany Department (Plant Pathology), Faculty of Agriculture, Damietta UniversityMolecular Biology Department, National Research CentreMolecular Biology Department, National Research CentrePlant Pathology Department, National Research CentrePlant Pathology Department, National Research CentreAbstract Background Faba bean attacked by soil-borne pathogens causing root rot disease. This disease has serious damage to both plant stand and produced yield. The present study aimed to evaluate effectiveness of the bioagents; Trichoderma harzianum and some plant resistance inducers as fungicide alternatives against root rot disease incidence at both pre- and post-emergence growth stages. Results Under open greenhouse conditions, the incidence of faba bean root rot in pre- and post-emergence growth phases was considerably reduced by using six inorganic salts and five antioxidants individually or combining with each other or with the bio-stimulator T. harzianum that exceeded the used fungicide, Rhizolex-T. Application of enervit agitated the highest significant defensive impact during pre-emergence stage versus root rot incidence (5.0%), followed by calcium sulfate and [cysteine + T. harzianum] (6.7%). At post-emergence stage, majority of the treatments completely suppressed (100.0%) root rot incidence, except vitamax plus and the fungicide (Rizolex-T) which expressed by 91.7 and 18.8%, respectively. Duplicate irrigations of 23 treatments after faba bean dressing improved the synthesis of different protein contents with the 2nd of which enhanced higher protein contents than the 1st one, except [T. harzianum + vitamin E + vitamin C + enervit + selenium + vitamax plus], [T. harzianum + vitamax plus] and cysteine. Disodium phosphate induced the highest catalase (CAT) activity (1820.8 and 1677.2 U/g FWt) after both irrigations. [T. harzianum + vitamax plus] and vitamin E induced the highest peroxidase (POD) activity 217.4 and 356.9 U/g FWt after 1st and 2nd irrigations, respectively. Disodium phosphate and [T. harzianum + vitamin E + vitamin C + enervit + selenium + vitamax plus] induced the highest chitinase (CHIA) activity 52.8 and 54.4 U/g FWt after 1st and 2nd irrigations, respectively. Application of disodium phosphate, calcium sulfate, potassium metabisulfite, sodium sulfate, cysteine, [cysteine + potash alum], enervit, vitamin E, [vitamin E + vitamin C + enervit + selenium + vitamax plus], [T. harzianum + enervit], [T. harzianum + selenium], [T. harzianum + vitamin E], [T. harzianum + vitamin E + vitamin C + enervit + selenium + vitamax plus] and vitamin C stimulated the formation of new protein bands on SDS-PAGE after the 2nd irrigation treatment. Conclusions Such treatments are considered good and environmentally safe alternatives against root diseases for getting rid of the negative effects of fungicides.https://doi.org/10.1186/s41938-023-00709-9AntioxidantsBiochemical studiesFaba beanInorganic saltsRoot rotTrichoderma harzianum |
| spellingShingle | Hassan Mohamed Masoud Allam Arafat Megahed Mohamed Salah Eldin Helmy Mahmoud Abdel-Aziz Ibrahim Nehal Samy El-Mougy Mokhtar Mohamed Abdel-Kader Phytopathological and biochemical impacts of Trichoderma harzianum and certain plant resistance inducers on faba bean root rot disease Egyptian Journal of Biological Pest Control Antioxidants Biochemical studies Faba bean Inorganic salts Root rot Trichoderma harzianum |
| title | Phytopathological and biochemical impacts of Trichoderma harzianum and certain plant resistance inducers on faba bean root rot disease |
| title_full | Phytopathological and biochemical impacts of Trichoderma harzianum and certain plant resistance inducers on faba bean root rot disease |
| title_fullStr | Phytopathological and biochemical impacts of Trichoderma harzianum and certain plant resistance inducers on faba bean root rot disease |
| title_full_unstemmed | Phytopathological and biochemical impacts of Trichoderma harzianum and certain plant resistance inducers on faba bean root rot disease |
| title_short | Phytopathological and biochemical impacts of Trichoderma harzianum and certain plant resistance inducers on faba bean root rot disease |
| title_sort | phytopathological and biochemical impacts of trichoderma harzianum and certain plant resistance inducers on faba bean root rot disease |
| topic | Antioxidants Biochemical studies Faba bean Inorganic salts Root rot Trichoderma harzianum |
| url | https://doi.org/10.1186/s41938-023-00709-9 |
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