Isolation, Identification and Antimicrobial Activities of Four Bacteria Associated with Entomopathogenic Nematodes, Steinernema spp.
Recently, the search for novel products derived from natural sources has become necessary due to the decreasing effectiveness of current antibiotics in treating bacteria that are antibiotic-resistant. In this context, it is well known that entomopathogenic bacteria (EPBs) produce a broad range of se...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2023-03-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/isolation-identification-and-antimicrobial-activities-of-four-bacteria-associated-with-entomopathogenic-nematodes-steinernema-spp/ |
| _version_ | 1797868075240914944 |
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| author | Othman M. Alzahrani |
| author_facet | Othman M. Alzahrani |
| author_sort | Othman M. Alzahrani |
| collection | DOAJ |
| description | Recently, the search for novel products derived from natural sources has become necessary due to the decreasing effectiveness of current antibiotics in treating bacteria that are antibiotic-resistant. In this context, it is well known that entomopathogenic bacteria (EPBs) produce a broad range of secondary metabolites with antibacterial activities. Therefore, an in-vitro trial was conducted to isolate and identify non-symbiotic bacteria associated with entomopathogenic nematodes, Steinernema spp. and evaluate the antibacterial activity against four antibiotic-resistant bacteria. Four bacterial isolates, i.e. Pseudomonas alcaligenes ST-1, Paenibacillus barcinonensis ST-2, Bacillus mojavensis ST-3, and Bacillus megaterium ST-4 were non-symbiotically isolated from the heamolymph of dead Steinernema-infected Galleria mellonella larvae and molecularly characterized. The bacterial cells and filtrates from P. alcaligenes ST-1 strongly inhibited the growth of Staphylococcus aureus through disk diffusion (43 mm), minimum inhibitory concentration (2.5 µL/mL), and minimal bactericidal concentration (5 µL/mL) assay. Conclusively, the direct application of endogenous Steinernema-associated EPB as an antibacterial agent for antibiotic-resistant bacteria looked promising. |
| first_indexed | 2024-04-09T23:50:14Z |
| format | Article |
| id | doaj.art-6755f40a87454c6482fabe01c66c3d39 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-04-09T23:50:14Z |
| publishDate | 2023-03-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-6755f40a87454c6482fabe01c66c3d392023-03-17T11:14:57ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2023-03-01171309319https://doi.org/10.22207/JPAM.17.1.20Isolation, Identification and Antimicrobial Activities of Four Bacteria Associated with Entomopathogenic Nematodes, Steinernema spp.Othman M. Alzahranihttps://orcid.org/0000-0001-6684-1257Recently, the search for novel products derived from natural sources has become necessary due to the decreasing effectiveness of current antibiotics in treating bacteria that are antibiotic-resistant. In this context, it is well known that entomopathogenic bacteria (EPBs) produce a broad range of secondary metabolites with antibacterial activities. Therefore, an in-vitro trial was conducted to isolate and identify non-symbiotic bacteria associated with entomopathogenic nematodes, Steinernema spp. and evaluate the antibacterial activity against four antibiotic-resistant bacteria. Four bacterial isolates, i.e. Pseudomonas alcaligenes ST-1, Paenibacillus barcinonensis ST-2, Bacillus mojavensis ST-3, and Bacillus megaterium ST-4 were non-symbiotically isolated from the heamolymph of dead Steinernema-infected Galleria mellonella larvae and molecularly characterized. The bacterial cells and filtrates from P. alcaligenes ST-1 strongly inhibited the growth of Staphylococcus aureus through disk diffusion (43 mm), minimum inhibitory concentration (2.5 µL/mL), and minimal bactericidal concentration (5 µL/mL) assay. Conclusively, the direct application of endogenous Steinernema-associated EPB as an antibacterial agent for antibiotic-resistant bacteria looked promising.https://microbiologyjournal.org/isolation-identification-and-antimicrobial-activities-of-four-bacteria-associated-with-entomopathogenic-nematodes-steinernema-spp/non-symbiotic entomopathogenic bacteriamolecular identificationantibiotic-resistant bacteriaantibacterial activity |
| spellingShingle | Othman M. Alzahrani Isolation, Identification and Antimicrobial Activities of Four Bacteria Associated with Entomopathogenic Nematodes, Steinernema spp. Journal of Pure and Applied Microbiology non-symbiotic entomopathogenic bacteria molecular identification antibiotic-resistant bacteria antibacterial activity |
| title | Isolation, Identification and Antimicrobial Activities of Four Bacteria Associated with Entomopathogenic Nematodes, Steinernema spp. |
| title_full | Isolation, Identification and Antimicrobial Activities of Four Bacteria Associated with Entomopathogenic Nematodes, Steinernema spp. |
| title_fullStr | Isolation, Identification and Antimicrobial Activities of Four Bacteria Associated with Entomopathogenic Nematodes, Steinernema spp. |
| title_full_unstemmed | Isolation, Identification and Antimicrobial Activities of Four Bacteria Associated with Entomopathogenic Nematodes, Steinernema spp. |
| title_short | Isolation, Identification and Antimicrobial Activities of Four Bacteria Associated with Entomopathogenic Nematodes, Steinernema spp. |
| title_sort | isolation identification and antimicrobial activities of four bacteria associated with entomopathogenic nematodes steinernema spp |
| topic | non-symbiotic entomopathogenic bacteria molecular identification antibiotic-resistant bacteria antibacterial activity |
| url | https://microbiologyjournal.org/isolation-identification-and-antimicrobial-activities-of-four-bacteria-associated-with-entomopathogenic-nematodes-steinernema-spp/ |
| work_keys_str_mv | AT othmanmalzahrani isolationidentificationandantimicrobialactivitiesoffourbacteriaassociatedwithentomopathogenicnematodessteinernemaspp |