Vitamin D receptor expression in sperms of male with unexplained infertility
Background: Vitamin D is associated with numerous disorders, including infertility. Accordingly, the goal of this research was to find out the level of vitamin D and messenger RNA (mRNA) expression of vitamin D receptor (VDR) in the sperm of male subjects with unexplained infertility. Methods: Twent...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Babol University of Medical Sciences
2023-01-01
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| Series: | Caspian Journal of Internal Medicine |
| Subjects: | |
| Online Access: | http://caspjim.com/article-1-3047-en.html |
| _version_ | 1797957159514800128 |
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| author | Zahra Akbari Sagheb Mousa Mohammadnia-Afrouzi Hamid Shafi Sedigheh Esmaelzadeh Kousar Smailnejad Ganji Mehdi Shahbazi |
| author_facet | Zahra Akbari Sagheb Mousa Mohammadnia-Afrouzi Hamid Shafi Sedigheh Esmaelzadeh Kousar Smailnejad Ganji Mehdi Shahbazi |
| author_sort | Zahra Akbari Sagheb |
| collection | DOAJ |
| description | Background: Vitamin D is associated with numerous disorders, including infertility. Accordingly, the goal of this research was to find out the level of vitamin D and messenger RNA (mRNA) expression of vitamin D receptor (VDR) in the sperm of male subjects with unexplained infertility.
Methods: Twenty-four unexplained infertile men as the case group and 22 healthy fertile men as the control group were recruited. Vitamin D levels were evaluated in seminal fluid using enzyme-linked immunosorbent assay (ELISA). Afterwards, the swim-up test was performed to isolate motile sperm cells. From these cells, RNA was extracted, complementary DNA (cDNA) was synthesized, and mRNA expression of the VDR gene was evaluated with quantitative real-time polymerase chain reaction (PCR).
Results: A decrease in VDR mRNA expression levels was detected in the case group compared to the control group, but this reduction was not statistically significant (p>0.05). Besides, the level of vitamin D in seminal fluid was not detectable in both groups.
Conclusion: The sperm of unexplained infertile men express VDR gene mRNA, although there was no vitamin D in seminal samples. Hence, vitamin D and VDR signaling might not be effective in the etiopathogenesis of unexplained infertility in men. |
| first_indexed | 2024-04-10T23:59:57Z |
| format | Article |
| id | doaj.art-6757043e6f3c4a5c9c53e56ae8e20216 |
| institution | Directory Open Access Journal |
| issn | 2008-6164 2008-6172 |
| language | English |
| last_indexed | 2024-04-10T23:59:57Z |
| publishDate | 2023-01-01 |
| publisher | Babol University of Medical Sciences |
| record_format | Article |
| series | Caspian Journal of Internal Medicine |
| spelling | doaj.art-6757043e6f3c4a5c9c53e56ae8e202162023-01-10T05:28:09ZengBabol University of Medical SciencesCaspian Journal of Internal Medicine2008-61642008-61722023-01-011419499Vitamin D receptor expression in sperms of male with unexplained infertilityZahra Akbari Sagheb0Mousa Mohammadnia-Afrouzi1Hamid Shafi2Sedigheh Esmaelzadeh3Kousar Smailnejad Ganji4Mehdi Shahbazi5 Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran Immunoregulation Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran Background: Vitamin D is associated with numerous disorders, including infertility. Accordingly, the goal of this research was to find out the level of vitamin D and messenger RNA (mRNA) expression of vitamin D receptor (VDR) in the sperm of male subjects with unexplained infertility. Methods: Twenty-four unexplained infertile men as the case group and 22 healthy fertile men as the control group were recruited. Vitamin D levels were evaluated in seminal fluid using enzyme-linked immunosorbent assay (ELISA). Afterwards, the swim-up test was performed to isolate motile sperm cells. From these cells, RNA was extracted, complementary DNA (cDNA) was synthesized, and mRNA expression of the VDR gene was evaluated with quantitative real-time polymerase chain reaction (PCR). Results: A decrease in VDR mRNA expression levels was detected in the case group compared to the control group, but this reduction was not statistically significant (p>0.05). Besides, the level of vitamin D in seminal fluid was not detectable in both groups. Conclusion: The sperm of unexplained infertile men express VDR gene mRNA, although there was no vitamin D in seminal samples. Hence, vitamin D and VDR signaling might not be effective in the etiopathogenesis of unexplained infertility in men.http://caspjim.com/article-1-3047-en.htmlvitamin d receptorunexplained infertilityvitamin dmrna expression |
| spellingShingle | Zahra Akbari Sagheb Mousa Mohammadnia-Afrouzi Hamid Shafi Sedigheh Esmaelzadeh Kousar Smailnejad Ganji Mehdi Shahbazi Vitamin D receptor expression in sperms of male with unexplained infertility Caspian Journal of Internal Medicine vitamin d receptor unexplained infertility vitamin d mrna expression |
| title | Vitamin D receptor expression in sperms of male with unexplained infertility |
| title_full | Vitamin D receptor expression in sperms of male with unexplained infertility |
| title_fullStr | Vitamin D receptor expression in sperms of male with unexplained infertility |
| title_full_unstemmed | Vitamin D receptor expression in sperms of male with unexplained infertility |
| title_short | Vitamin D receptor expression in sperms of male with unexplained infertility |
| title_sort | vitamin d receptor expression in sperms of male with unexplained infertility |
| topic | vitamin d receptor unexplained infertility vitamin d mrna expression |
| url | http://caspjim.com/article-1-3047-en.html |
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