Uncertainty Estimation for Quantitative Agarose Gel Electrophoresis of Nucleic Acids
This paper considers the evaluation of uncertainty of quantitative gel electrophoresis. To date, such uncertainty estimation presented in the literature are based on the multiple measurements performed for assessing the intra- and interlaboratory reproducibility using standard samples. This paper sh...
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| Format: | Članak |
| Jezik: | English |
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MDPI AG
2023-02-01
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| Serija: | Sensors |
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| Online pristup: | https://www.mdpi.com/1424-8220/23/4/1999 |
| _version_ | 1827755542361145344 |
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| author | Konstantin Semenov Aleksandr Taraskin Alexandra Yurchenko Irina Baranovskaya Lada Purvinsh Natalia Gyulikhandanova Andrey Vasin |
| author_facet | Konstantin Semenov Aleksandr Taraskin Alexandra Yurchenko Irina Baranovskaya Lada Purvinsh Natalia Gyulikhandanova Andrey Vasin |
| author_sort | Konstantin Semenov |
| collection | DOAJ |
| description | This paper considers the evaluation of uncertainty of quantitative gel electrophoresis. To date, such uncertainty estimation presented in the literature are based on the multiple measurements performed for assessing the intra- and interlaboratory reproducibility using standard samples. This paper shows how to estimate the uncertainty in cases where we cannot study scattering components of the results. The first point is dedicated to a case where we have standard samples (the direct expressions are shown). The second point considers the situation when standard samples are absent (the algorithm for estimating the lower bound for uncertainty is discussed). The role of the data processing algorithm is demonstrated. |
| first_indexed | 2024-03-11T08:10:49Z |
| format | Article |
| id | doaj.art-67571959df1a4602b486f48c0fcce328 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-11T08:10:49Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-67571959df1a4602b486f48c0fcce3282023-11-16T23:08:57ZengMDPI AGSensors1424-82202023-02-01234199910.3390/s23041999Uncertainty Estimation for Quantitative Agarose Gel Electrophoresis of Nucleic AcidsKonstantin Semenov0Aleksandr Taraskin1Alexandra Yurchenko2Irina Baranovskaya3Lada Purvinsh4Natalia Gyulikhandanova5Andrey Vasin6Peter the Great St. Petersburg Polytechnic University, 29, Polytechnicheskaya Str., 195251 St. Petersburg, RussiaPeter the Great St. Petersburg Polytechnic University, 29, Polytechnicheskaya Str., 195251 St. Petersburg, RussiaPeter the Great St. Petersburg Polytechnic University, 29, Polytechnicheskaya Str., 195251 St. Petersburg, RussiaAugusta University, 1120 15th Str., Augusta, GA 30912, USAThe University of Chicago, 947 E. 58th Str., Chicago, IL 60637, USAPeter the Great St. Petersburg Polytechnic University, 29, Polytechnicheskaya Str., 195251 St. Petersburg, RussiaPeter the Great St. Petersburg Polytechnic University, 29, Polytechnicheskaya Str., 195251 St. Petersburg, RussiaThis paper considers the evaluation of uncertainty of quantitative gel electrophoresis. To date, such uncertainty estimation presented in the literature are based on the multiple measurements performed for assessing the intra- and interlaboratory reproducibility using standard samples. This paper shows how to estimate the uncertainty in cases where we cannot study scattering components of the results. The first point is dedicated to a case where we have standard samples (the direct expressions are shown). The second point considers the situation when standard samples are absent (the algorithm for estimating the lower bound for uncertainty is discussed). The role of the data processing algorithm is demonstrated.https://www.mdpi.com/1424-8220/23/4/1999quantitative gel electrophoresiselectrophoretic separationnucleic acidsmetrological analysisuncertainty propagationinaccurate data processing |
| spellingShingle | Konstantin Semenov Aleksandr Taraskin Alexandra Yurchenko Irina Baranovskaya Lada Purvinsh Natalia Gyulikhandanova Andrey Vasin Uncertainty Estimation for Quantitative Agarose Gel Electrophoresis of Nucleic Acids Sensors quantitative gel electrophoresis electrophoretic separation nucleic acids metrological analysis uncertainty propagation inaccurate data processing |
| title | Uncertainty Estimation for Quantitative Agarose Gel Electrophoresis of Nucleic Acids |
| title_full | Uncertainty Estimation for Quantitative Agarose Gel Electrophoresis of Nucleic Acids |
| title_fullStr | Uncertainty Estimation for Quantitative Agarose Gel Electrophoresis of Nucleic Acids |
| title_full_unstemmed | Uncertainty Estimation for Quantitative Agarose Gel Electrophoresis of Nucleic Acids |
| title_short | Uncertainty Estimation for Quantitative Agarose Gel Electrophoresis of Nucleic Acids |
| title_sort | uncertainty estimation for quantitative agarose gel electrophoresis of nucleic acids |
| topic | quantitative gel electrophoresis electrophoretic separation nucleic acids metrological analysis uncertainty propagation inaccurate data processing |
| url | https://www.mdpi.com/1424-8220/23/4/1999 |
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