Role of Angiopoietic Coronary Endothelial Dysfunction in the Pathogenesis of Ischemic Cardiomyopathy
Background: The angiopoietic endothelial dysfunction in ischemic cardiomyopathy (ICMP) remains unexplored. Aim: The identification of the imbalance of endothelial dysfunction mediators and the number of endothelial progenitor (EPC) and desquamated (EDC) cells in patients with coronary heart disease...
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MDPI AG
2023-07-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/7/1950 |
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| author | Svetlana P. Chumakova Olga I. Urazova Vladimir M. Shipulin Sergey L. Andreev Olga A. Denisenko Margarita V. Gladkovskaya Larisa S. Litvinova Mikhail A. Bubenchikov |
| author_facet | Svetlana P. Chumakova Olga I. Urazova Vladimir M. Shipulin Sergey L. Andreev Olga A. Denisenko Margarita V. Gladkovskaya Larisa S. Litvinova Mikhail A. Bubenchikov |
| author_sort | Svetlana P. Chumakova |
| collection | DOAJ |
| description | Background: The angiopoietic endothelial dysfunction in ischemic cardiomyopathy (ICMP) remains unexplored. Aim: The identification of the imbalance of endothelial dysfunction mediators and the number of endothelial progenitor (EPC) and desquamated (EDC) cells in patients with coronary heart disease (CHD) with and without ICMP. Methods: A total of 87 patients (47 with ICMP and 40 without ICMP) were observed. The content of EPCs (CD14<sup>+</sup>CD34<sup>+</sup>VEGFR2<sup>+</sup>) in vein blood and EDCs (CD45<sup>−</sup>CD146<sup>+</sup>) in the blood from the coronary sinus and cubital vein was determined by flow cytometry. The contents of HIF-1α and HIF-2α in vein blood as well as that of ADMA and endothelin-1 in sinus plasma and angiopoietin-2, MMP-9 and galectin-3 in both samples were assessed using ELISA, and VEGF, PDGF, SDF-1 and MCP-1 contents using immunofluorescence. Results: ADMA and endothelin-1 levels in the sinus blood were comparable between the patient groups; a deficiency of HIF-1α and excess of HIF-2α were detected in the vein blood of ICMP patients. The EDC content in the vein blood increased in CHD patients regardless of ICMP, and the concentrations of VEGF-A, VEGF-B, PDGF, MCP-1, angiopoietin-2, and MMP-9 were normal. In ICMP patients, vein blood was characterized by an excess of galectin-3 and sinus blood by an excess of EDCs, angiopoietin-2, MMP-9 and galectin-3. Conclusion: ICMP is accompanied by angiopoietic endothelial dysfunction. |
| first_indexed | 2024-03-11T01:16:38Z |
| format | Article |
| id | doaj.art-6757250b2cc0434e9c65df25950d9e04 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-11T01:16:38Z |
| publishDate | 2023-07-01 |
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| series | Biomedicines |
| spelling | doaj.art-6757250b2cc0434e9c65df25950d9e042023-11-18T18:27:18ZengMDPI AGBiomedicines2227-90592023-07-01117195010.3390/biomedicines11071950Role of Angiopoietic Coronary Endothelial Dysfunction in the Pathogenesis of Ischemic CardiomyopathySvetlana P. Chumakova0Olga I. Urazova1Vladimir M. Shipulin2Sergey L. Andreev3Olga A. Denisenko4Margarita V. Gladkovskaya5Larisa S. Litvinova6Mikhail A. Bubenchikov7Pathophysiology Division, Siberian State Medical University, Tomsk 634050, RussiaPathophysiology Division, Siberian State Medical University, Tomsk 634050, RussiaCardiovascular Surgery Unit, Cardiology Research Institute, Tomsk National Medical Research Center, Russian Academy of Sciences, Tomsk 634050, RussiaCardiovascular Surgery Unit, Cardiology Research Institute, Tomsk National Medical Research Center, Russian Academy of Sciences, Tomsk 634050, RussiaPathophysiology Division, Siberian State Medical University, Tomsk 634050, RussiaPathophysiology Division, Siberian State Medical University, Tomsk 634050, RussiaImmunology and Cell Biotechnology Center, Immanuel Kant Baltic Federal University, Kaliningrad 236041, RussiaDepartment of Theoretical Mechanics, National Research Tomsk State University, Tomsk 634050, RussiaBackground: The angiopoietic endothelial dysfunction in ischemic cardiomyopathy (ICMP) remains unexplored. Aim: The identification of the imbalance of endothelial dysfunction mediators and the number of endothelial progenitor (EPC) and desquamated (EDC) cells in patients with coronary heart disease (CHD) with and without ICMP. Methods: A total of 87 patients (47 with ICMP and 40 without ICMP) were observed. The content of EPCs (CD14<sup>+</sup>CD34<sup>+</sup>VEGFR2<sup>+</sup>) in vein blood and EDCs (CD45<sup>−</sup>CD146<sup>+</sup>) in the blood from the coronary sinus and cubital vein was determined by flow cytometry. The contents of HIF-1α and HIF-2α in vein blood as well as that of ADMA and endothelin-1 in sinus plasma and angiopoietin-2, MMP-9 and galectin-3 in both samples were assessed using ELISA, and VEGF, PDGF, SDF-1 and MCP-1 contents using immunofluorescence. Results: ADMA and endothelin-1 levels in the sinus blood were comparable between the patient groups; a deficiency of HIF-1α and excess of HIF-2α were detected in the vein blood of ICMP patients. The EDC content in the vein blood increased in CHD patients regardless of ICMP, and the concentrations of VEGF-A, VEGF-B, PDGF, MCP-1, angiopoietin-2, and MMP-9 were normal. In ICMP patients, vein blood was characterized by an excess of galectin-3 and sinus blood by an excess of EDCs, angiopoietin-2, MMP-9 and galectin-3. Conclusion: ICMP is accompanied by angiopoietic endothelial dysfunction.https://www.mdpi.com/2227-9059/11/7/1950angiogenesisgrowth factorsendothelial progenitor cellscoronary heart diseaseheart failure |
| spellingShingle | Svetlana P. Chumakova Olga I. Urazova Vladimir M. Shipulin Sergey L. Andreev Olga A. Denisenko Margarita V. Gladkovskaya Larisa S. Litvinova Mikhail A. Bubenchikov Role of Angiopoietic Coronary Endothelial Dysfunction in the Pathogenesis of Ischemic Cardiomyopathy Biomedicines angiogenesis growth factors endothelial progenitor cells coronary heart disease heart failure |
| title | Role of Angiopoietic Coronary Endothelial Dysfunction in the Pathogenesis of Ischemic Cardiomyopathy |
| title_full | Role of Angiopoietic Coronary Endothelial Dysfunction in the Pathogenesis of Ischemic Cardiomyopathy |
| title_fullStr | Role of Angiopoietic Coronary Endothelial Dysfunction in the Pathogenesis of Ischemic Cardiomyopathy |
| title_full_unstemmed | Role of Angiopoietic Coronary Endothelial Dysfunction in the Pathogenesis of Ischemic Cardiomyopathy |
| title_short | Role of Angiopoietic Coronary Endothelial Dysfunction in the Pathogenesis of Ischemic Cardiomyopathy |
| title_sort | role of angiopoietic coronary endothelial dysfunction in the pathogenesis of ischemic cardiomyopathy |
| topic | angiogenesis growth factors endothelial progenitor cells coronary heart disease heart failure |
| url | https://www.mdpi.com/2227-9059/11/7/1950 |
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