Inhibitory Activity of HEp-2 Cells by Honey from Indonesia
Indonesian local honey contains active compounds that have the potential as an antioxidant and anticancer, primarily as a laryngeal anticancer through the inhibition of HEp-2 cells. This study aims to determine the anticancer activity of several types of honey in Indonesia through the inhibition of...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University
2019-11-01
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| Series: | Jurnal Kimia Sains dan Aplikasi |
| Subjects: | |
| Online Access: | https://ejournal.undip.ac.id/index.php/ksa/article/view/24103 |
| _version_ | 1819284780423839744 |
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| author | La Ode Sumarlin Anna Muawanah Farhan Riza Afandi Adawiah Adawiah |
| author_facet | La Ode Sumarlin Anna Muawanah Farhan Riza Afandi Adawiah Adawiah |
| author_sort | La Ode Sumarlin |
| collection | DOAJ |
| description | Indonesian local honey contains active compounds that have the potential as an antioxidant and anticancer, primarily as a laryngeal anticancer through the inhibition of HEp-2 cells. This study aims to determine the anticancer activity of several types of honey in Indonesia through the inhibition of HEp-2 cells. Samples used in the form of Trigona, Longan, Rambutan, and Kaliandra honey obtained from honey farmers in Sulawesi and Java, Indonesia. Honey samples extracted by using methanol, then liquids partition was carried out consecutively using n-hexane and ethyl acetate. The cytotoxicity test for HEp-2 cells was carried out using the MTT method (3- (4,5-dimethyl thiazol-2-il) -2,5-diphenyl tetrazolium bromide). The results showed that all honey samples were active against preventing HEp-2 cells with the highest inhibition activity from longan honey with ethyl acetate extract at 65.18% at 100 ppm. Longan honey has decreased HEp-2 cell inhibitory activity after fractionation. Indonesian local honey, namely trigona honey, kaliandra honey, rambutan honey, and longan honey, can be used as a supplementary supplement for patients with laryngeal cancer. |
| first_indexed | 2024-12-24T01:52:49Z |
| format | Article |
| id | doaj.art-67594c6c3c7447d1893249b3d9575eed |
| institution | Directory Open Access Journal |
| issn | 1410-8917 2597-9914 |
| language | English |
| last_indexed | 2024-12-24T01:52:49Z |
| publishDate | 2019-11-01 |
| publisher | Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University |
| record_format | Article |
| series | Jurnal Kimia Sains dan Aplikasi |
| spelling | doaj.art-67594c6c3c7447d1893249b3d9575eed2022-12-21T17:21:41ZengChemistry Department, Faculty of Sciences and Mathematics, Diponegoro UniversityJurnal Kimia Sains dan Aplikasi1410-89172597-99142019-11-0122631732510.14710/jksa.22.6.317-32515792Inhibitory Activity of HEp-2 Cells by Honey from IndonesiaLa Ode Sumarlin0Anna Muawanah1Farhan Riza Afandi2Adawiah Adawiah3Chemistry Study Program, Syarif Hidayatullah State Islamic University, IndonesiaChemistry Study Program, Syarif Hidayatullah State Islamic University, IndonesiaChemistry Study Program, Syarif Hidayatullah State Islamic University, IndonesiaChemistry Study Program, Syarif Hidayatullah State Islamic University, IndonesiaIndonesian local honey contains active compounds that have the potential as an antioxidant and anticancer, primarily as a laryngeal anticancer through the inhibition of HEp-2 cells. This study aims to determine the anticancer activity of several types of honey in Indonesia through the inhibition of HEp-2 cells. Samples used in the form of Trigona, Longan, Rambutan, and Kaliandra honey obtained from honey farmers in Sulawesi and Java, Indonesia. Honey samples extracted by using methanol, then liquids partition was carried out consecutively using n-hexane and ethyl acetate. The cytotoxicity test for HEp-2 cells was carried out using the MTT method (3- (4,5-dimethyl thiazol-2-il) -2,5-diphenyl tetrazolium bromide). The results showed that all honey samples were active against preventing HEp-2 cells with the highest inhibition activity from longan honey with ethyl acetate extract at 65.18% at 100 ppm. Longan honey has decreased HEp-2 cell inhibitory activity after fractionation. Indonesian local honey, namely trigona honey, kaliandra honey, rambutan honey, and longan honey, can be used as a supplementary supplement for patients with laryngeal cancer.https://ejournal.undip.ac.id/index.php/ksa/article/view/24103anticancerhoneymtt assayinhibitoryhep-2 cell |
| spellingShingle | La Ode Sumarlin Anna Muawanah Farhan Riza Afandi Adawiah Adawiah Inhibitory Activity of HEp-2 Cells by Honey from Indonesia Jurnal Kimia Sains dan Aplikasi anticancer honey mtt assay inhibitory hep-2 cell |
| title | Inhibitory Activity of HEp-2 Cells by Honey from Indonesia |
| title_full | Inhibitory Activity of HEp-2 Cells by Honey from Indonesia |
| title_fullStr | Inhibitory Activity of HEp-2 Cells by Honey from Indonesia |
| title_full_unstemmed | Inhibitory Activity of HEp-2 Cells by Honey from Indonesia |
| title_short | Inhibitory Activity of HEp-2 Cells by Honey from Indonesia |
| title_sort | inhibitory activity of hep 2 cells by honey from indonesia |
| topic | anticancer honey mtt assay inhibitory hep-2 cell |
| url | https://ejournal.undip.ac.id/index.php/ksa/article/view/24103 |
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