Endogenous Expression of G-CSF in Rat Dorsal Root Ganglion Neurons after Nerve Injury
Granulocyte colony-stimulating factor (G-CSF) has been reported to modulate pain function following nerve injury. However, the expression of endogenous G-CSF in the dorsal root ganglion (DRG) and the response to nerve injury remain unclear. In the present study, we demonstrated that G-CSF and G-CSFR...
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MDPI AG
2021-07-01
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| Series: | Brain Sciences |
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| Online Access: | https://www.mdpi.com/2076-3425/11/7/956 |
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| author | Chun-Chang Yeh Chih-Ping Yang Kuo-Hsing Ma Jui-Hu Shih Ching-San Tseng Yuahn-Sieh Huang |
| author_facet | Chun-Chang Yeh Chih-Ping Yang Kuo-Hsing Ma Jui-Hu Shih Ching-San Tseng Yuahn-Sieh Huang |
| author_sort | Chun-Chang Yeh |
| collection | DOAJ |
| description | Granulocyte colony-stimulating factor (G-CSF) has been reported to modulate pain function following nerve injury. However, the expression of endogenous G-CSF in the dorsal root ganglion (DRG) and the response to nerve injury remain unclear. In the present study, we demonstrated that G-CSF and G-CSFR are mainly expressed in both small- and medium-diameter DRG neurons in rats and are responsible for transmitting pain responses. G-CSF and G-CSFR were co-expressed in certain nociceptive DRG neurons. In addition, G-CSF was expressed in satellite glial cells around large-diameter DRG neurons. After sciatic nerve injury, the number of G-CSF-positive DRG neurons was increased in both the ipsilateral and contralateral lesion sites in rats. However, G-CSF expression in satellite glial cells was not affected by nerve injury. To clarify the role of G-CSF in pain, exogenous G-CSF was administered to a rat model of neuropathic pain induced by partial sciatic nerve transaction (PST). Our results indicate that treatment with G-CSF did not attenuate but exacerbated neuropathic pain. In summary, G-CSF may directly activate sensory neurons and contribute to nociceptive signaling. |
| first_indexed | 2024-03-10T09:44:02Z |
| format | Article |
| id | doaj.art-675a7c6d003e41b1b1f6662aaa64c151 |
| institution | Directory Open Access Journal |
| issn | 2076-3425 |
| language | English |
| last_indexed | 2024-03-10T09:44:02Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-675a7c6d003e41b1b1f6662aaa64c1512023-11-22T03:21:33ZengMDPI AGBrain Sciences2076-34252021-07-0111795610.3390/brainsci11070956Endogenous Expression of G-CSF in Rat Dorsal Root Ganglion Neurons after Nerve InjuryChun-Chang Yeh0Chih-Ping Yang1Kuo-Hsing Ma2Jui-Hu Shih3Ching-San Tseng4Yuahn-Sieh Huang5Department of Anesthesiology, National Defense Medical Center, Tri-Service General Hospital, Taipei 11490, TaiwanDepartment of Anesthesiology, National Defense Medical Center, Tri-Service General Hospital, Taipei 11490, TaiwanDepartment of Biology and Anatomy, National Defense Medical Center, Taipei 11490, TaiwanDepartment of Pharmacy Practice, Tri-Service General Hospital, Taipei 11490, TaiwanDepartment of Biology and Anatomy, National Defense Medical Center, Taipei 11490, TaiwanDepartment of Biology and Anatomy, National Defense Medical Center, Taipei 11490, TaiwanGranulocyte colony-stimulating factor (G-CSF) has been reported to modulate pain function following nerve injury. However, the expression of endogenous G-CSF in the dorsal root ganglion (DRG) and the response to nerve injury remain unclear. In the present study, we demonstrated that G-CSF and G-CSFR are mainly expressed in both small- and medium-diameter DRG neurons in rats and are responsible for transmitting pain responses. G-CSF and G-CSFR were co-expressed in certain nociceptive DRG neurons. In addition, G-CSF was expressed in satellite glial cells around large-diameter DRG neurons. After sciatic nerve injury, the number of G-CSF-positive DRG neurons was increased in both the ipsilateral and contralateral lesion sites in rats. However, G-CSF expression in satellite glial cells was not affected by nerve injury. To clarify the role of G-CSF in pain, exogenous G-CSF was administered to a rat model of neuropathic pain induced by partial sciatic nerve transaction (PST). Our results indicate that treatment with G-CSF did not attenuate but exacerbated neuropathic pain. In summary, G-CSF may directly activate sensory neurons and contribute to nociceptive signaling.https://www.mdpi.com/2076-3425/11/7/956G-CSFG-CSFRDRGneuropathic pain |
| spellingShingle | Chun-Chang Yeh Chih-Ping Yang Kuo-Hsing Ma Jui-Hu Shih Ching-San Tseng Yuahn-Sieh Huang Endogenous Expression of G-CSF in Rat Dorsal Root Ganglion Neurons after Nerve Injury Brain Sciences G-CSF G-CSFR DRG neuropathic pain |
| title | Endogenous Expression of G-CSF in Rat Dorsal Root Ganglion Neurons after Nerve Injury |
| title_full | Endogenous Expression of G-CSF in Rat Dorsal Root Ganglion Neurons after Nerve Injury |
| title_fullStr | Endogenous Expression of G-CSF in Rat Dorsal Root Ganglion Neurons after Nerve Injury |
| title_full_unstemmed | Endogenous Expression of G-CSF in Rat Dorsal Root Ganglion Neurons after Nerve Injury |
| title_short | Endogenous Expression of G-CSF in Rat Dorsal Root Ganglion Neurons after Nerve Injury |
| title_sort | endogenous expression of g csf in rat dorsal root ganglion neurons after nerve injury |
| topic | G-CSF G-CSFR DRG neuropathic pain |
| url | https://www.mdpi.com/2076-3425/11/7/956 |
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