PSGL-1 is a novel tumor microenvironment prognostic biomarker with cervical high-grade squamous lesions and more
BackgroundMacrophages secrete many cytokines and chemokines, which can provoke either an anti-tumor or pro-tumor immune response. P-selectin glycoprotein ligand-1 (PSGL-1) is expressed in macrophages and plays a vital role in synergizing for a more robust anti-tumor response. However, there are few...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2023.1052201/full |
| _version_ | 1811158377332473856 |
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| author | Yingying Lin Shan Huang Yuanjie Qi Li Xie Junying Jiang Hua Li Zhiwei Chen |
| author_facet | Yingying Lin Shan Huang Yuanjie Qi Li Xie Junying Jiang Hua Li Zhiwei Chen |
| author_sort | Yingying Lin |
| collection | DOAJ |
| description | BackgroundMacrophages secrete many cytokines and chemokines, which can provoke either an anti-tumor or pro-tumor immune response. P-selectin glycoprotein ligand-1 (PSGL-1) is expressed in macrophages and plays a vital role in synergizing for a more robust anti-tumor response. However, there are few studies about PSGL-1 expression status and clinical value of biological function in cervical cancer.MethodsIn this study, 565 participants were enrolled. PSGL-1 mRNA was detected by real-time quantitative PCR (qPCR) with cervical cytology specimens. The relationship between PSGL-1 and cervical intraepithelial neoplasia in two grades and more (CIN2+) was analyzed, and the optimal cut-off values of PSGL-1 to predict CIN2+ were calculated. In addition, the clinical significance of PSGL-1 in cervical cancer was determined by Kaplan-Meier Cox regression based on the database.ResultsThe mean PSGL-1 increased significantly with cervical lesion development, especially compared with CIN2+ (p<0.05). Moreover, the expression of PSGL-1 increased significantly in HPV-16/18 positive and HPV-18 positive, but not in HPV-16 positive and other HR-HPV positive. And then, it demonstrated that the area under the receiver operating characteristic curve (AUC) of PSGL-1 was 0.820, and an optimal cut-off 0.245. Furthermore, the PSGL-1 had the highest odds ratio and highest OR (OR= 8.707; 95% CI (.371-19.321)) for the detection of CIN 2+. In addition, our result also indicated that higher PSGL-1 expression was significantly related to a better prognosis in cervical cancer due to immune cell infiltration.ConclusionsPSGL-1≥0.245 in cervical cytology specimens is a new auxiliary biomarker of CIN2+, and it may be a promising prognosis predictor and potential immunotherapy target linked with immune infiltration of cervical cancer. |
| first_indexed | 2024-04-10T05:23:38Z |
| format | Article |
| id | doaj.art-675b82101dcf4f85ba343b88e660ca36 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-10T05:23:38Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-675b82101dcf4f85ba343b88e660ca362023-03-08T04:53:00ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2023-03-011310.3389/fonc.2023.10522011052201PSGL-1 is a novel tumor microenvironment prognostic biomarker with cervical high-grade squamous lesions and moreYingying Lin0Shan Huang1Yuanjie Qi2Li Xie3Junying Jiang4Hua Li5Zhiwei Chen6Department of Healthcare, Fujian Maternity and Child Health Hospital, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou, ChinaDepartment of Traditional Chinese Medicine, Fujian Maternity and Child Health Hospital, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou, ChinaDepartment of Gynecologic, Fujian Maternity and Child Health Hospital, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou, ChinaDepartment of Gynecologic, Fujian Maternity and Child Health Hospital, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou, ChinaDepartment of Gynecologic, Fujian Maternity and Child Health Hospital, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou, ChinaDepartment of Gynecologic, Fujian Maternity and Child Health Hospital, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou, ChinaDepartment of Gynecologic, Fujian Maternity and Child Health Hospital, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou, ChinaBackgroundMacrophages secrete many cytokines and chemokines, which can provoke either an anti-tumor or pro-tumor immune response. P-selectin glycoprotein ligand-1 (PSGL-1) is expressed in macrophages and plays a vital role in synergizing for a more robust anti-tumor response. However, there are few studies about PSGL-1 expression status and clinical value of biological function in cervical cancer.MethodsIn this study, 565 participants were enrolled. PSGL-1 mRNA was detected by real-time quantitative PCR (qPCR) with cervical cytology specimens. The relationship between PSGL-1 and cervical intraepithelial neoplasia in two grades and more (CIN2+) was analyzed, and the optimal cut-off values of PSGL-1 to predict CIN2+ were calculated. In addition, the clinical significance of PSGL-1 in cervical cancer was determined by Kaplan-Meier Cox regression based on the database.ResultsThe mean PSGL-1 increased significantly with cervical lesion development, especially compared with CIN2+ (p<0.05). Moreover, the expression of PSGL-1 increased significantly in HPV-16/18 positive and HPV-18 positive, but not in HPV-16 positive and other HR-HPV positive. And then, it demonstrated that the area under the receiver operating characteristic curve (AUC) of PSGL-1 was 0.820, and an optimal cut-off 0.245. Furthermore, the PSGL-1 had the highest odds ratio and highest OR (OR= 8.707; 95% CI (.371-19.321)) for the detection of CIN 2+. In addition, our result also indicated that higher PSGL-1 expression was significantly related to a better prognosis in cervical cancer due to immune cell infiltration.ConclusionsPSGL-1≥0.245 in cervical cytology specimens is a new auxiliary biomarker of CIN2+, and it may be a promising prognosis predictor and potential immunotherapy target linked with immune infiltration of cervical cancer.https://www.frontiersin.org/articles/10.3389/fonc.2023.1052201/fullPSGL-1cervical cancerprognosistumor-infiltrating-immune cellsimmunotherapy |
| spellingShingle | Yingying Lin Shan Huang Yuanjie Qi Li Xie Junying Jiang Hua Li Zhiwei Chen PSGL-1 is a novel tumor microenvironment prognostic biomarker with cervical high-grade squamous lesions and more Frontiers in Oncology PSGL-1 cervical cancer prognosis tumor-infiltrating-immune cells immunotherapy |
| title | PSGL-1 is a novel tumor microenvironment prognostic biomarker with cervical high-grade squamous lesions and more |
| title_full | PSGL-1 is a novel tumor microenvironment prognostic biomarker with cervical high-grade squamous lesions and more |
| title_fullStr | PSGL-1 is a novel tumor microenvironment prognostic biomarker with cervical high-grade squamous lesions and more |
| title_full_unstemmed | PSGL-1 is a novel tumor microenvironment prognostic biomarker with cervical high-grade squamous lesions and more |
| title_short | PSGL-1 is a novel tumor microenvironment prognostic biomarker with cervical high-grade squamous lesions and more |
| title_sort | psgl 1 is a novel tumor microenvironment prognostic biomarker with cervical high grade squamous lesions and more |
| topic | PSGL-1 cervical cancer prognosis tumor-infiltrating-immune cells immunotherapy |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2023.1052201/full |
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