Comparison of the Efficacy of <i>Trichoderma</i> and <i>Bacillus</i> Strains and Commercial Biocontrol Products against Grapevine <i>Botryosphaeria</i> Dieback Pathogens
Grapevine trunk diseases (GTDs) cause significant yield losses worldwide and limit the lifespan of vineyards. In the last few years, using biological control agents (BCAs) for pruning wound protection has become a promising management strategy for the control of these pathologies. This study aimed t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/2/533 |
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| author | Natalia Langa-Lomba Vicente González-García M. Eugenia Venturini-Crespo José Casanova-Gascón Juan J. Barriuso-Vargas Pablo Martín-Ramos |
| author_facet | Natalia Langa-Lomba Vicente González-García M. Eugenia Venturini-Crespo José Casanova-Gascón Juan J. Barriuso-Vargas Pablo Martín-Ramos |
| author_sort | Natalia Langa-Lomba |
| collection | DOAJ |
| description | Grapevine trunk diseases (GTDs) cause significant yield losses worldwide and limit the lifespan of vineyards. In the last few years, using biological control agents (BCAs) for pruning wound protection has become a promising management strategy for the control of these pathologies. This study aimed to compare the antifungal activities of a grapevine-native <i>Trichoderma harzianum</i> isolate and a high-potential <i>Bacillus velezensis</i> strain against two pathogenic <i>Botryosphaeriaceae</i> species in artificially inoculated, potted, grafted plants under controlled greenhouse conditions, taking three commercial biocontrol products (based on <i>T. atroviride</i> I-1237, <i>T. harzianum</i> T-22, and <i>Bacillus subtilis</i> BS03 strains) as a reference. To reproduce certain field conditions more realistically, inoculation of the protective agents and the pathogens was conducted simultaneously immediately after pruning instead of allowing the BCAs to colonize the wounds before pathogen inoculation. Significant differences in necrosis lengths were detected for both <i>Neofusicoccum parvum</i>- and <i>Diplodia seriata</i>-infected plants, and a remarkable protective effect of <i>Bacillus velezensis</i> BUZ-14 was observed in all cases. <i>Trichoderma</i>-based treatments showed different efficacies against the two pathogenic fungi. While the three tested BCAs resulted in significant reductions in vascular necrosis caused by <i>N. parvum</i>, they did not significantly reduce <i>D. seriata</i> infection compared to the untreated inoculated control. The <i>B. subtilis</i> strain was not effective. The reported results provide support for the potential <i>Bacillus velezensis</i> may have for pruning wound protection against <i>Botryosphaeriaceae</i> fungi, encouraging its evaluation under natural field conditions. |
| first_indexed | 2024-03-11T09:16:59Z |
| format | Article |
| id | doaj.art-6761badd159d4fff9e91fc44c5160b02 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-11T09:16:59Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-6761badd159d4fff9e91fc44c5160b022023-11-16T18:36:01ZengMDPI AGAgronomy2073-43952023-02-0113253310.3390/agronomy13020533Comparison of the Efficacy of <i>Trichoderma</i> and <i>Bacillus</i> Strains and Commercial Biocontrol Products against Grapevine <i>Botryosphaeria</i> Dieback PathogensNatalia Langa-Lomba0Vicente González-García1M. Eugenia Venturini-Crespo2José Casanova-Gascón3Juan J. Barriuso-Vargas4Pablo Martín-Ramos5Department of Agricultural, Forest and Environmental Systems, Agrifood Research and Technology Centre of Aragón, Instituto Agroalimentario de Aragón—IA2 (Universidad de Zaragoza-CITA), Avda. Montañana 930, 50059 Zaragoza, SpainDepartment of Agricultural, Forest and Environmental Systems, Agrifood Research and Technology Centre of Aragón, Instituto Agroalimentario de Aragón—IA2 (Universidad de Zaragoza-CITA), Avda. Montañana 930, 50059 Zaragoza, SpainPlant Food Research Group, Instituto Agroalimentario de Aragón—IA2 (Universidad de Zaragoza-CITA), Miguel Servet 177, 50013 Zaragoza, SpainInstituto Agroalimentario de Aragón-IA2 (Universidad de Zaragoza-CITA), Avda. Montañana 930, 50059 Zaragoza, SpainInstituto Agroalimentario de Aragón-IA2 (Universidad de Zaragoza-CITA), Avda. Montañana 930, 50059 Zaragoza, SpainInstituto Universitario de Investigación en Ciencias Ambientales de Aragón (IUCA), EPS, University of Zaragoza, Carretera de Cuarte s/n, 22071 Huesca, SpainGrapevine trunk diseases (GTDs) cause significant yield losses worldwide and limit the lifespan of vineyards. In the last few years, using biological control agents (BCAs) for pruning wound protection has become a promising management strategy for the control of these pathologies. This study aimed to compare the antifungal activities of a grapevine-native <i>Trichoderma harzianum</i> isolate and a high-potential <i>Bacillus velezensis</i> strain against two pathogenic <i>Botryosphaeriaceae</i> species in artificially inoculated, potted, grafted plants under controlled greenhouse conditions, taking three commercial biocontrol products (based on <i>T. atroviride</i> I-1237, <i>T. harzianum</i> T-22, and <i>Bacillus subtilis</i> BS03 strains) as a reference. To reproduce certain field conditions more realistically, inoculation of the protective agents and the pathogens was conducted simultaneously immediately after pruning instead of allowing the BCAs to colonize the wounds before pathogen inoculation. Significant differences in necrosis lengths were detected for both <i>Neofusicoccum parvum</i>- and <i>Diplodia seriata</i>-infected plants, and a remarkable protective effect of <i>Bacillus velezensis</i> BUZ-14 was observed in all cases. <i>Trichoderma</i>-based treatments showed different efficacies against the two pathogenic fungi. While the three tested BCAs resulted in significant reductions in vascular necrosis caused by <i>N. parvum</i>, they did not significantly reduce <i>D. seriata</i> infection compared to the untreated inoculated control. The <i>B. subtilis</i> strain was not effective. The reported results provide support for the potential <i>Bacillus velezensis</i> may have for pruning wound protection against <i>Botryosphaeriaceae</i> fungi, encouraging its evaluation under natural field conditions.https://www.mdpi.com/2073-4395/13/2/533<i>Bacillus subtilis</i><i>Bacillus velezensis</i>BCAs<i>Diplodia seriata</i>GTDs<i>Neofusicoccum parvum</i> |
| spellingShingle | Natalia Langa-Lomba Vicente González-García M. Eugenia Venturini-Crespo José Casanova-Gascón Juan J. Barriuso-Vargas Pablo Martín-Ramos Comparison of the Efficacy of <i>Trichoderma</i> and <i>Bacillus</i> Strains and Commercial Biocontrol Products against Grapevine <i>Botryosphaeria</i> Dieback Pathogens Agronomy <i>Bacillus subtilis</i> <i>Bacillus velezensis</i> BCAs <i>Diplodia seriata</i> GTDs <i>Neofusicoccum parvum</i> |
| title | Comparison of the Efficacy of <i>Trichoderma</i> and <i>Bacillus</i> Strains and Commercial Biocontrol Products against Grapevine <i>Botryosphaeria</i> Dieback Pathogens |
| title_full | Comparison of the Efficacy of <i>Trichoderma</i> and <i>Bacillus</i> Strains and Commercial Biocontrol Products against Grapevine <i>Botryosphaeria</i> Dieback Pathogens |
| title_fullStr | Comparison of the Efficacy of <i>Trichoderma</i> and <i>Bacillus</i> Strains and Commercial Biocontrol Products against Grapevine <i>Botryosphaeria</i> Dieback Pathogens |
| title_full_unstemmed | Comparison of the Efficacy of <i>Trichoderma</i> and <i>Bacillus</i> Strains and Commercial Biocontrol Products against Grapevine <i>Botryosphaeria</i> Dieback Pathogens |
| title_short | Comparison of the Efficacy of <i>Trichoderma</i> and <i>Bacillus</i> Strains and Commercial Biocontrol Products against Grapevine <i>Botryosphaeria</i> Dieback Pathogens |
| title_sort | comparison of the efficacy of i trichoderma i and i bacillus i strains and commercial biocontrol products against grapevine i botryosphaeria i dieback pathogens |
| topic | <i>Bacillus subtilis</i> <i>Bacillus velezensis</i> BCAs <i>Diplodia seriata</i> GTDs <i>Neofusicoccum parvum</i> |
| url | https://www.mdpi.com/2073-4395/13/2/533 |
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