Dual RNA-Seq Unveils Pseudomonas plecoglossicida htpG Gene Functions During Host-Pathogen Interactions With Epinephelus coioides
Pseudomonas plecoglossicida is a temperature-dependent opportunistic pathogen which is associated with a variety of diseases in fish. During the development of “white nodules” disease, the expression of htpG in P. plecoglossicida was found to be significantly up-regulated at its virulent temperature...
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Frontiers Media S.A.
2019-05-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2019.00984/full |
| _version_ | 1819046962586976256 |
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| author | Lixing Huang Lingmin Zhao Wenjia Liu Xiaojin Xu Yongquan Su Yingxue Qin Qingpi Yan Qingpi Yan |
| author_facet | Lixing Huang Lingmin Zhao Wenjia Liu Xiaojin Xu Yongquan Su Yingxue Qin Qingpi Yan Qingpi Yan |
| author_sort | Lixing Huang |
| collection | DOAJ |
| description | Pseudomonas plecoglossicida is a temperature-dependent opportunistic pathogen which is associated with a variety of diseases in fish. During the development of “white nodules” disease, the expression of htpG in P. plecoglossicida was found to be significantly up-regulated at its virulent temperature of 18°C. The infection of htpG-RNAi strain resulted in the onset time delay, reduction in mortality and infection symptoms in spleen of Epinephelus coioides, and affected the bacterial tissue colonization. In order to reveal the effect of htpG silencing of P. plecoglossicida on the virulence regulation in P. plecoglossicida and immune response in E. coioides, dual RNA-seq was performed and a pathogen-host integration network was constructed. Our results showed that infection induced the expression of host genes related to immune response, but attenuated the expression of bacterial virulence genes. Novel integration was found between host immune genes and bacterial virulence genes, while IL6, IL1R2, IL1B, and TLR5 played key roles in the network. Further analysis with GeneMANIA indicated that flgD and rplF might play key roles during the htpG-dependent virulence regulation, which was in accordance with the reduced biofilm production, motility and virulence in htpG-RNAi strain. Meanwhile, IL6 and IL1B were found to play key roles during the defense against P. plecoglossicida, while CELA2, TRY, CPA1, CPA2, and CPB1 were important targets for P. plecoglossicida attacking to the host. |
| first_indexed | 2024-12-21T10:52:48Z |
| format | Article |
| id | doaj.art-67645416a9fb49ac9cf1624e1105f57c |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-21T10:52:48Z |
| publishDate | 2019-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-67645416a9fb49ac9cf1624e1105f57c2022-12-21T19:06:36ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-05-011010.3389/fimmu.2019.00984447806Dual RNA-Seq Unveils Pseudomonas plecoglossicida htpG Gene Functions During Host-Pathogen Interactions With Epinephelus coioidesLixing Huang0Lingmin Zhao1Wenjia Liu2Xiaojin Xu3Yongquan Su4Yingxue Qin5Qingpi Yan6Qingpi Yan7Key Laboratory of Healthy Mariculture for the East China Sea, Fisheries College, Jimei University, Ministry of Agriculture, Xiamen, ChinaKey Laboratory of Healthy Mariculture for the East China Sea, Fisheries College, Jimei University, Ministry of Agriculture, Xiamen, ChinaKey Laboratory of Healthy Mariculture for the East China Sea, Fisheries College, Jimei University, Ministry of Agriculture, Xiamen, ChinaKey Laboratory of Healthy Mariculture for the East China Sea, Fisheries College, Jimei University, Ministry of Agriculture, Xiamen, ChinaState Key Laboratory of Large Yellow Croaker Breeding, Ningde, ChinaKey Laboratory of Healthy Mariculture for the East China Sea, Fisheries College, Jimei University, Ministry of Agriculture, Xiamen, ChinaKey Laboratory of Healthy Mariculture for the East China Sea, Fisheries College, Jimei University, Ministry of Agriculture, Xiamen, ChinaState Key Laboratory of Large Yellow Croaker Breeding, Ningde, ChinaPseudomonas plecoglossicida is a temperature-dependent opportunistic pathogen which is associated with a variety of diseases in fish. During the development of “white nodules” disease, the expression of htpG in P. plecoglossicida was found to be significantly up-regulated at its virulent temperature of 18°C. The infection of htpG-RNAi strain resulted in the onset time delay, reduction in mortality and infection symptoms in spleen of Epinephelus coioides, and affected the bacterial tissue colonization. In order to reveal the effect of htpG silencing of P. plecoglossicida on the virulence regulation in P. plecoglossicida and immune response in E. coioides, dual RNA-seq was performed and a pathogen-host integration network was constructed. Our results showed that infection induced the expression of host genes related to immune response, but attenuated the expression of bacterial virulence genes. Novel integration was found between host immune genes and bacterial virulence genes, while IL6, IL1R2, IL1B, and TLR5 played key roles in the network. Further analysis with GeneMANIA indicated that flgD and rplF might play key roles during the htpG-dependent virulence regulation, which was in accordance with the reduced biofilm production, motility and virulence in htpG-RNAi strain. Meanwhile, IL6 and IL1B were found to play key roles during the defense against P. plecoglossicida, while CELA2, TRY, CPA1, CPA2, and CPB1 were important targets for P. plecoglossicida attacking to the host.https://www.frontiersin.org/article/10.3389/fimmu.2019.00984/fullpathogen-host molecular integrationdual RNA-seqPseudomonas plecoglossicidaEpinephelus coioideshtpG |
| spellingShingle | Lixing Huang Lingmin Zhao Wenjia Liu Xiaojin Xu Yongquan Su Yingxue Qin Qingpi Yan Qingpi Yan Dual RNA-Seq Unveils Pseudomonas plecoglossicida htpG Gene Functions During Host-Pathogen Interactions With Epinephelus coioides Frontiers in Immunology pathogen-host molecular integration dual RNA-seq Pseudomonas plecoglossicida Epinephelus coioides htpG |
| title | Dual RNA-Seq Unveils Pseudomonas plecoglossicida htpG Gene Functions During Host-Pathogen Interactions With Epinephelus coioides |
| title_full | Dual RNA-Seq Unveils Pseudomonas plecoglossicida htpG Gene Functions During Host-Pathogen Interactions With Epinephelus coioides |
| title_fullStr | Dual RNA-Seq Unveils Pseudomonas plecoglossicida htpG Gene Functions During Host-Pathogen Interactions With Epinephelus coioides |
| title_full_unstemmed | Dual RNA-Seq Unveils Pseudomonas plecoglossicida htpG Gene Functions During Host-Pathogen Interactions With Epinephelus coioides |
| title_short | Dual RNA-Seq Unveils Pseudomonas plecoglossicida htpG Gene Functions During Host-Pathogen Interactions With Epinephelus coioides |
| title_sort | dual rna seq unveils pseudomonas plecoglossicida htpg gene functions during host pathogen interactions with epinephelus coioides |
| topic | pathogen-host molecular integration dual RNA-seq Pseudomonas plecoglossicida Epinephelus coioides htpG |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2019.00984/full |
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